open access

Vol 82, No 3 (2023)
Original article
Submitted: 2022-02-23
Accepted: 2022-06-06
Published online: 2022-06-10
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Expression and distribution of erythropoietin, vascular endothelial growth factor (VEGF) and VEGF receptor 2 in small intestine of yaks at different ages

Y. Zhou1, T. Zhang1, Y. Y. Zhang123, J. Xu1, M. Li1, Q. Zhang4, Z. Qiao23, K. Yang123
·
Pubmed: 35692112
·
Folia Morphol 2023;82(3):683-695.
Affiliations
  1. Life Science and Engineering College, Northwest Minzu University, Lanzhou, P.R. China
  2. Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Centre, Northwest Minzu University, Lanzhou, P.R. China
  3. Gansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Lanzhou, P.R. China
  4. Laboratory of Animal Anatomy and Tissue Embryology, Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou, P.R. China

open access

Vol 82, No 3 (2023)
ORIGINAL ARTICLES
Submitted: 2022-02-23
Accepted: 2022-06-06
Published online: 2022-06-10

Abstract

Background: This study aimed to detect the expression and distribution of vascular
endothelial growth factor (VEGF), VEGF receptor 2 (VEGFR-2), and erythropoietin
(EPO) proteins in small intestinal tissues of 50-day-old, newborn, and adult yaks.
The results provide basic data for the study of the relationship between adaptability
and age of plateau yak.
Materials and methods: Small intestine tissues from healthy adult, 50-day-old,
and newborn yak were collected and embedded in paraffin sections. Histological
features were observed by haematoxylin and eosin staining. The expression of
VEGF, VEGFR-2, and EPO proteins were detected by immunohistochemical staining.
Results: Immunohistochemical results showed that of the expression VEGF,
VEGFR-2, and EPO were detected in the small intestinal villi of yaks at all ages.
The EPO expression level in the jejunum and duodenal villous epithelial cells of
newborn yaks was significantly higher than that of 50-day-old and adult yaks.
The EPO expression level in ileum villous epithelial cells of 50-day-old yaks was
significantly higher than that of newborn and adult yaks. VEGF expression in
newborn yak ileum and jejunum epithelial cells of the intestinal villus were significantly
higher than in the 50-day-old and adult. In the 50-day-old yaks, the
duodenal intestinal villus epithelial cells expression levels were higher than in the
adult and newborn yaks. The expression level of VEGFR-2 in the ileum, jejunum
and duodenal villous epithelial cells of 50-day-old yak was significantly higher
than in that of adult and newborn yak.
Conclusions: The expression and distribution characteristics of EPO, VEGF, and
VEGFR-2 in yak intestinal tissues of different ages indicate that these proteins
may be involved in the physiological regulation of yak intestines in hypoxic environments.
It may be an important regulatory protein in yak adaptation to a high
altitude and low oxygen environment.

Abstract

Background: This study aimed to detect the expression and distribution of vascular
endothelial growth factor (VEGF), VEGF receptor 2 (VEGFR-2), and erythropoietin
(EPO) proteins in small intestinal tissues of 50-day-old, newborn, and adult yaks.
The results provide basic data for the study of the relationship between adaptability
and age of plateau yak.
Materials and methods: Small intestine tissues from healthy adult, 50-day-old,
and newborn yak were collected and embedded in paraffin sections. Histological
features were observed by haematoxylin and eosin staining. The expression of
VEGF, VEGFR-2, and EPO proteins were detected by immunohistochemical staining.
Results: Immunohistochemical results showed that of the expression VEGF,
VEGFR-2, and EPO were detected in the small intestinal villi of yaks at all ages.
The EPO expression level in the jejunum and duodenal villous epithelial cells of
newborn yaks was significantly higher than that of 50-day-old and adult yaks.
The EPO expression level in ileum villous epithelial cells of 50-day-old yaks was
significantly higher than that of newborn and adult yaks. VEGF expression in
newborn yak ileum and jejunum epithelial cells of the intestinal villus were significantly
higher than in the 50-day-old and adult. In the 50-day-old yaks, the
duodenal intestinal villus epithelial cells expression levels were higher than in the
adult and newborn yaks. The expression level of VEGFR-2 in the ileum, jejunum
and duodenal villous epithelial cells of 50-day-old yak was significantly higher
than in that of adult and newborn yak.
Conclusions: The expression and distribution characteristics of EPO, VEGF, and
VEGFR-2 in yak intestinal tissues of different ages indicate that these proteins
may be involved in the physiological regulation of yak intestines in hypoxic environments.
It may be an important regulatory protein in yak adaptation to a high
altitude and low oxygen environment.

Get Citation

Keywords

yak, intestine, growth, protein expression

About this article
Title

Expression and distribution of erythropoietin, vascular endothelial growth factor (VEGF) and VEGF receptor 2 in small intestine of yaks at different ages

Journal

Folia Morphologica

Issue

Vol 82, No 3 (2023)

Article type

Original article

Pages

683-695

Published online

2022-06-10

Page views

1019

Article views/downloads

676

DOI

10.5603/FM.a2022.0058

Pubmed

35692112

Bibliographic record

Folia Morphol 2023;82(3):683-695.

Keywords

yak
intestine
growth
protein expression

Authors

Y. Zhou
T. Zhang
Y. Y. Zhang
J. Xu
M. Li
Q. Zhang
Z. Qiao
K. Yang

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