open access

Vol 82, No 2 (2023)
Original article
Submitted: 2022-02-07
Accepted: 2022-02-09
Published online: 2022-02-28
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The relationship between the aggrecan VNTR polymorphism and its content in lumbar intervertebral discs

P. Pękala1, D. Felkle2, K. Dykas2, A. Jarosz3, P. Elnazir1, T. Konopka4, J. A. Walocha1, J. Dulinska-Litewka2
·
Pubmed: 35239182
·
Folia Morphol 2023;82(2):269-273.
Affiliations
  1. Department of Anatomy, Jagiellonian University Medical College, Krakow, Poland
  2. Department of Biochemistry, Jagiellonian University Medical College, Krakow, Poland
  3. Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland
  4. Department of Forensic Medicine, Jagiellonian University Medical College, Krakow, Poland

open access

Vol 82, No 2 (2023)
ORIGINAL ARTICLES
Submitted: 2022-02-07
Accepted: 2022-02-09
Published online: 2022-02-28

Abstract

Background: There is a specific polymorphism of the ACAN gene called the variable
number of tandem repeats (VNTR), which is particularly interesting in the light
of the development of intervertebral disc pathology and associated low back pain.
Materials and methods: The nucleus pulposus specimens were harvested from
the L5/S1 intervertebral discs. The aggrecan content was determined using enzyme-
linked immunosorbent assay (ELISA). Moreover, the VNTR polymorphism
in the ACAN gene was evaluated.
Results: The genotyping of VNTR polymorphism in ACAN gene was successful
in 94 tissue samples (48 homozygotes and 46 heterozygotes). The alleles were
divided into four groups, in accordance with the number of tandem repeats in
the ACAN gene. No difference between groups in the mean aggrecan mass nor
in the mean degree of tissue moisture was observed.
Conclusions: No relationship between the ACAN gene VNTR polymorphism and
the aggrecan content was observed in studied Caucasian cadavers. Such a relationship
may be a more complex phenomenon and exists in other populations.

Abstract

Background: There is a specific polymorphism of the ACAN gene called the variable
number of tandem repeats (VNTR), which is particularly interesting in the light
of the development of intervertebral disc pathology and associated low back pain.
Materials and methods: The nucleus pulposus specimens were harvested from
the L5/S1 intervertebral discs. The aggrecan content was determined using enzyme-
linked immunosorbent assay (ELISA). Moreover, the VNTR polymorphism
in the ACAN gene was evaluated.
Results: The genotyping of VNTR polymorphism in ACAN gene was successful
in 94 tissue samples (48 homozygotes and 46 heterozygotes). The alleles were
divided into four groups, in accordance with the number of tandem repeats in
the ACAN gene. No difference between groups in the mean aggrecan mass nor
in the mean degree of tissue moisture was observed.
Conclusions: No relationship between the ACAN gene VNTR polymorphism and
the aggrecan content was observed in studied Caucasian cadavers. Such a relationship
may be a more complex phenomenon and exists in other populations.

Get Citation

Keywords

aggrecan, intervertebral disc, morphology, risk factor

About this article
Title

The relationship between the aggrecan VNTR polymorphism and its content in lumbar intervertebral discs

Journal

Folia Morphologica

Issue

Vol 82, No 2 (2023)

Article type

Original article

Pages

269-273

Published online

2022-02-28

Page views

2366

Article views/downloads

806

DOI

10.5603/FM.a2022.0019

Pubmed

35239182

Bibliographic record

Folia Morphol 2023;82(2):269-273.

Keywords

aggrecan
intervertebral disc
morphology
risk factor

Authors

P. Pękala
D. Felkle
K. Dykas
A. Jarosz
P. Elnazir
T. Konopka
J. A. Walocha
J. Dulinska-Litewka

References (17)
  1. Antoniou J, Steffen T, Nelson F, et al. The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration. J Clin Invest. 1996; 98(4): 996–1003.
  2. Balagué F, Mannion AF, Pellisé F, et al. Non-specific low back pain. Lancet. 2012; 379(9814): 482–491.
  3. Biczo A, Szita J, McCall I, et al. Genodisc Consortium. Association of vitamin D receptor gene polymorphisms with disc degeneration. Eur Spine J. 2020; 29(3): 596–604.
  4. Cong L, Pang H, Xuan D, et al. The interaction between aggrecan gene VNTR polymorphism and cigarette smoking in predicting incident symptomatic intervertebral disc degeneration. Connect Tissue Res. 2010; 51(5): 397–403.
  5. Cong L, Tu G, Liang D. A systematic review of the relationship between the distributions of aggrecan gene VNTR polymorphism and degenerative disc disease/osteoarthritis. Bone Joint Res. 2018; 7(4): 308–317.
  6. Cong L, Zhu Y, Pang H, et al. The interaction between aggrecan gene VNTR polymorphism and obesity in predicting incident symptomatic lumbar disc herniation. Connect Tissue Res. 2014; 55(5-6): 384–390.
  7. Gleinert-Rożek MŁ, Kosiński A, Kaczyńska A, et al. Metric analysis of the lumbar region of human vertebral column. Folia Morphol. 2020; 79(4): 655–661.
  8. Gu J, Guan F, Guan G, et al. Aggrecan variable number of tandem repeat polymorphism and lumbar disc degeneration: a meta-analysis. Spine (Phila Pa 1976). 2013; 38(25): E1600–E1607.
  9. Hoy D, Brooks P, Blyth F, et al. The Epidemiology of low back pain. Best Pract Res Clin Rheumatol. 2010; 24(6): 769–781.
  10. Kapetanakis S, Gkantsinikoudis N. Anatomy of lumbar facet joint: a comprehensive review. Folia Morphol. 2021; 80(4): 799–805.
  11. Maldonado M, Nam J. The role of changes in extracellular matrix of cartilage in the presence of inflammation on the pathology of osteoarthritis. Biomed Res Int. 2013; 2013: 284873.
  12. Pearce RH, Thompson P, Bebault GM, et al. Magnetic resonance imaging reflects changes of ageing degeneration in the human intervertebral disc. J Rheumatol. 1991; 18(Suppl. 27): 42–43.
  13. Pekala PA, Henry BM, Taterra D, et al. FokI as a genetic factor of intervertebral disc degeneration: A PRISMA-compliant systematic review of overlapping meta-analyses. J Clin Neurosci. 2019; 60: 36–43.
  14. Pękala P, Taterra D, Krupa K, et al. Correlation of morphological and radiological characteristics of degenerative disc disease in lumbar spine: a cadaveric study. Folia Morphol. 2022; 81(2): 503–509.
  15. Roughley P, Martens D, Rantakokko J, et al. The involvement of aggrecan polymorphism in degeneration of human intervertebral disc and articular cartilage. Eur Cell Mater. 2006; 11: 1–7.
  16. Urban JPG, Roberts S. Degeneration of the intervertebral disc. Arthritis Res Ther. 2003; 5(3): 120–130.
  17. Will JS, Bury DC, Miller JA. Mechanical low back pain. Am Fam Physician. 2018; 98(7): 421–428.

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