open access

Vol 81, No 4 (2022)
Original article
Submitted: 2021-08-23
Accepted: 2021-09-29
Published online: 2021-10-07
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Transmission electron microscopic investigation of the dark and light pancreatic acinar beta-cells of young-domesticated pig (Sus Suidae, Erxleben 1777)

M. Elghoul1, R. Kandyle2, K. Morsy34, M. M.A. Abumandour5
·
Pubmed: 34642928
·
Folia Morphol 2022;81(4):956-962.
Affiliations
  1. Department of Histology and Cytology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt
  2. Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
  3. Biology Department, Faculty of Science, King Khalid University, Abha, Saudi Arabia
  4. Zoology Department, Faculty of Science, Cairo University, Cairo, Egypt
  5. Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt

open access

Vol 81, No 4 (2022)
ORIGINAL ARTICLES
Submitted: 2021-08-23
Accepted: 2021-09-29
Published online: 2021-10-07

Abstract

Background: The current study was designed to perform a transmission electron microscopic investigation focusing on the dark and light pancreatic acinar β-cells of young domesticated pig (Sus Suidae).
Materials and methods: This study depended on the fresh pancreatic specimens from 5 healthy young (2-month-old) pigs that were collected immediately after they were slaughtered at the abattoir of Abdelkader Alexandria, Egypt.
Results and Conclusions: In our findings, the acinar pancreas was formed of pyramidal pancreatic acinar cells with large spherical nuclei of condensed heterochromatin at the periphery and prominent eccentric nucleoli. Zymogen granules were observed at the apical region of the acinar cells, and they appear as electron dense bodies. Numerous mitochondria and Golgi complexes observed in the acinar cell cytoplasm. The electron dense acinar cells were joined by junctional complexes. The rough endoplasmic reticulum was more prominent in the electron-dense acinar cells than did electron-lucent acinar cells. There was no connective tissue capsule separate the acinar portion of pancreas from the pancreatic islets. The pancreatic islets mainly formed of β-cells. The irregular α-cells possess numerous small granules. The cytoplasmic β-cells granules were surrounded by hallow area and enclosed by a limiting membrane. Delta cells were generally polygonal in shape and found in clumps throughout the islet and they were also identified in between β-cells. Their granules were of moderate electron density and were generally smaller than β-cells’ granules. The limiting membrane was tightly enclosed the delta cells granules and the hallow area around the granule were found similar to the granules of β-cells.

Abstract

Background: The current study was designed to perform a transmission electron microscopic investigation focusing on the dark and light pancreatic acinar β-cells of young domesticated pig (Sus Suidae).
Materials and methods: This study depended on the fresh pancreatic specimens from 5 healthy young (2-month-old) pigs that were collected immediately after they were slaughtered at the abattoir of Abdelkader Alexandria, Egypt.
Results and Conclusions: In our findings, the acinar pancreas was formed of pyramidal pancreatic acinar cells with large spherical nuclei of condensed heterochromatin at the periphery and prominent eccentric nucleoli. Zymogen granules were observed at the apical region of the acinar cells, and they appear as electron dense bodies. Numerous mitochondria and Golgi complexes observed in the acinar cell cytoplasm. The electron dense acinar cells were joined by junctional complexes. The rough endoplasmic reticulum was more prominent in the electron-dense acinar cells than did electron-lucent acinar cells. There was no connective tissue capsule separate the acinar portion of pancreas from the pancreatic islets. The pancreatic islets mainly formed of β-cells. The irregular α-cells possess numerous small granules. The cytoplasmic β-cells granules were surrounded by hallow area and enclosed by a limiting membrane. Delta cells were generally polygonal in shape and found in clumps throughout the islet and they were also identified in between β-cells. Their granules were of moderate electron density and were generally smaller than β-cells’ granules. The limiting membrane was tightly enclosed the delta cells granules and the hallow area around the granule were found similar to the granules of β-cells.

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Keywords

pancreas, Sus Suidae, transmission electron microscope, pancreatic islets, pancreatic cells

About this article
Title

Transmission electron microscopic investigation of the dark and light pancreatic acinar beta-cells of young-domesticated pig (Sus Suidae, Erxleben 1777)

Journal

Folia Morphologica

Issue

Vol 81, No 4 (2022)

Article type

Original article

Pages

956-962

Published online

2021-10-07

Page views

4085

Article views/downloads

688

DOI

10.5603/FM.a2021.0104

Pubmed

34642928

Bibliographic record

Folia Morphol 2022;81(4):956-962.

Keywords

pancreas
Sus Suidae
transmission electron microscope
pancreatic islets
pancreatic cells

Authors

M. Elghoul
R. Kandyle
K. Morsy
M. M.A. Abumandour

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