open access

Vol 82, No 1 (2023)
Original article
Submitted: 2021-08-13
Accepted: 2021-11-05
Published online: 2022-01-17
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Anatomo-topographic and histo-cytological study of dromedary’s spleen in Algeria

M. A. Fares1, T. Khenenou1, D. E. Rahmoun2
·
Pubmed: 35099045
·
Folia Morphol 2023;82(1):137-146.
Affiliations
  1. Laboratory of Sciences and Technics of the Livings, Institute of Agriculture and Veterinary Sciences, Taoura, University of Souk Ahras, Algeria
  2. Laboratory of Animal Production, Biotechnologies and Health (PABIOS), Institute of Agriculture and Veterinary Sciences Taoura, University of Souk Ahras, Algeria

open access

Vol 82, No 1 (2023)
ORIGINAL ARTICLES
Submitted: 2021-08-13
Accepted: 2021-11-05
Published online: 2022-01-17

Abstract

Background: Twenty-five spleens of adult, healthy dromedary of the local breed from the region of El Oued, Algeria, were collected at the slaughterhouse in order to carry out research to determine the macroscopic and microscopic structure of spleen in this species, macroscopic study revealed that the spleen has a rectangular shape with a triangular section, rounded edges, a little bit striated, its surface is smooth in which the aspect of the capsule and the parietal surface is shiny and smooth, the morphometric study was carried out after classifying the sampled spleen in five groups according to the animal’s body weight which increases with age.
Materials and methods: Our study revealed that the groups show a different value of mass which declines towards a drop of the index, also the indexes of length and width are following a decreasing order.
Results: The histological study revealed that the zone occupied by stroma did not exceed 26.81% of the total components of the capsule which is composed essentially of connective tissue and an inner layer of smooth muscle cells. Vascular and avascular trabeculae extend from the capsule. The immunohistochemistry study made it possible to visualise T lymphocytes of the splenic parenchyma using monoclonal antibodies where a statistical study was carried out to determine the composition of the various compartments of this organ. The localisation of immunocompetent cells in the splenic parenchyma has been elucidated with antibodies specific for T lymphocytes.
Conclusions: The red pulp occupied a maximum area of the spleen with an average of 68.1% composed of sinusoids venous, the cords extend between the sinuses and the interlobular zone contain many cells: macrophages, plasma cells, red blood cells, white blood cells and platelets.

Abstract

Background: Twenty-five spleens of adult, healthy dromedary of the local breed from the region of El Oued, Algeria, were collected at the slaughterhouse in order to carry out research to determine the macroscopic and microscopic structure of spleen in this species, macroscopic study revealed that the spleen has a rectangular shape with a triangular section, rounded edges, a little bit striated, its surface is smooth in which the aspect of the capsule and the parietal surface is shiny and smooth, the morphometric study was carried out after classifying the sampled spleen in five groups according to the animal’s body weight which increases with age.
Materials and methods: Our study revealed that the groups show a different value of mass which declines towards a drop of the index, also the indexes of length and width are following a decreasing order.
Results: The histological study revealed that the zone occupied by stroma did not exceed 26.81% of the total components of the capsule which is composed essentially of connective tissue and an inner layer of smooth muscle cells. Vascular and avascular trabeculae extend from the capsule. The immunohistochemistry study made it possible to visualise T lymphocytes of the splenic parenchyma using monoclonal antibodies where a statistical study was carried out to determine the composition of the various compartments of this organ. The localisation of immunocompetent cells in the splenic parenchyma has been elucidated with antibodies specific for T lymphocytes.
Conclusions: The red pulp occupied a maximum area of the spleen with an average of 68.1% composed of sinusoids venous, the cords extend between the sinuses and the interlobular zone contain many cells: macrophages, plasma cells, red blood cells, white blood cells and platelets.

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Keywords

dromedary, lymphocytes, spleen, peri-arteriolar lymphoid sheath, red pulp, white pulp

About this article
Title

Anatomo-topographic and histo-cytological study of dromedary’s spleen in Algeria

Journal

Folia Morphologica

Issue

Vol 82, No 1 (2023)

Article type

Original article

Pages

137-146

Published online

2022-01-17

Page views

3295

Article views/downloads

1027

DOI

10.5603/FM.a2022.0004

Pubmed

35099045

Bibliographic record

Folia Morphol 2023;82(1):137-146.

Keywords

dromedary
lymphocytes
spleen
peri-arteriolar lymphoid sheath
red pulp
white pulp

Authors

M. A. Fares
T. Khenenou
D. E. Rahmoun

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