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ORIGINAL ARTICLES
Published online: 2020-07-29
Submitted: 2020-05-17
Accepted: 2020-06-30
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The ameliorative effect of curcumin on cryptorchid and non-cryptorchid testes in induced unilateral cryptorchidism in albino rat: histological evaluation

M. A. Abd-El-Hafez, M. D. El-Shafee, S. H. Omar, A. A. Aburahma, S. S. Kamar
DOI: 10.5603/FM.a2020.0084
·
Pubmed: 32748946

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2020-07-29
Submitted: 2020-05-17
Accepted: 2020-06-30

Abstract

Background: Cryptorchidism, one or both hidden testes, is the most common abnormality of male sexual development. Subfertility or infertility is associated with both unilateral and bilateral cryptorchidism. In this study, we investigate the possible ameliorative effect of curcumin (Cur) on the induced-unilateral cryptorchidism testicular injury in both cryptorchid (Cryp) and non-cryptorchid (non-Cryp) scrotal testes through histological, immunohistochemical and morphometrics.

Materials and methods: 40 adult male albino rats were divided into: control group, Cur control, Cryp group, and Cryp+Cur group. The rat model was surgically established by fixing the left testis in the abdomen. The treated groups were subjected to surgically induced-unilateral cryptorchidism on the left side then were given Cur (80 mg/kg) orally, for 20 days. Histological analysis using hematoxylin & eosin and periodic acid Schiff's reaction was applied. Immunohistochemistry was performed for proliferating cell nuclear antigen (PCNA); to estimate the proliferation in the germinal epithelium, and vimentin; to evaluate Sertoli cells. The results were confirmed by statistical evaluation of the spermatogenic epithelium height, the seminiferous tubules diameter, the basement membrane thickness, the number of PCNA immunostained cells and the area % of vimentin immunostaining.

Results: distorted seminefrous tubules, substantial  degeneration of the germinal epithelium,  thickening of the basement memebrane with a significant decrease in PCNA and vimentin immunostaining were observed in Cryp group; mainly in the cryptorchid testis. These structural changes were significantly reversed in Crypt+Cur group.

Conclusions: Curcumin proved to be an important and effective medical line for protecting against the unfavorable sequels of cryptorchidism in a rat model.

Abstract

Background: Cryptorchidism, one or both hidden testes, is the most common abnormality of male sexual development. Subfertility or infertility is associated with both unilateral and bilateral cryptorchidism. In this study, we investigate the possible ameliorative effect of curcumin (Cur) on the induced-unilateral cryptorchidism testicular injury in both cryptorchid (Cryp) and non-cryptorchid (non-Cryp) scrotal testes through histological, immunohistochemical and morphometrics.

Materials and methods: 40 adult male albino rats were divided into: control group, Cur control, Cryp group, and Cryp+Cur group. The rat model was surgically established by fixing the left testis in the abdomen. The treated groups were subjected to surgically induced-unilateral cryptorchidism on the left side then were given Cur (80 mg/kg) orally, for 20 days. Histological analysis using hematoxylin & eosin and periodic acid Schiff's reaction was applied. Immunohistochemistry was performed for proliferating cell nuclear antigen (PCNA); to estimate the proliferation in the germinal epithelium, and vimentin; to evaluate Sertoli cells. The results were confirmed by statistical evaluation of the spermatogenic epithelium height, the seminiferous tubules diameter, the basement membrane thickness, the number of PCNA immunostained cells and the area % of vimentin immunostaining.

Results: distorted seminefrous tubules, substantial  degeneration of the germinal epithelium,  thickening of the basement memebrane with a significant decrease in PCNA and vimentin immunostaining were observed in Cryp group; mainly in the cryptorchid testis. These structural changes were significantly reversed in Crypt+Cur group.

Conclusions: Curcumin proved to be an important and effective medical line for protecting against the unfavorable sequels of cryptorchidism in a rat model.

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Keywords

testis; unilateral cryptochidism; curcumin; PCNA; vimentin

About this article
Title

The ameliorative effect of curcumin on cryptorchid and non-cryptorchid testes in induced unilateral cryptorchidism in albino rat: histological evaluation

Journal

Folia Morphologica

Issue

Ahead of Print

Published online

2020-07-29

DOI

10.5603/FM.a2020.0084

Pubmed

32748946

Keywords

testis
unilateral cryptochidism
curcumin
PCNA
vimentin

Authors

M. A. Abd-El-Hafez
M. D. El-Shafee
S. H. Omar
A. A. Aburahma
S. S. Kamar

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