open access

Vol 80, No 3 (2021)
Original article
Submitted: 2020-05-17
Accepted: 2020-06-30
Published online: 2020-07-29
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The ameliorative effect of curcumin on cryptorchid and non-cryptorchid testes in induced unilateral cryptorchidism in albino rat: histological evaluation

M. A. Abd-El-Hafez1, M. D. El-Shafee1, S. H. Omar1, A. A. Aburahma2, S. S. Kamar1
·
Pubmed: 32748946
·
Folia Morphol 2021;80(3):596-604.
Affiliations
  1. Department of Medical Histology and Cell Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
  2. Department of Andrology, Faculty of Medicine, Cairo University, Cairo, Egypt

open access

Vol 80, No 3 (2021)
ORIGINAL ARTICLES
Submitted: 2020-05-17
Accepted: 2020-06-30
Published online: 2020-07-29

Abstract

Background: Cryptorchidism, one or both hidden testes, is the most common abnormality of male sexual development. Subfertility or infertility is associated with both unilateral and bilateral cryptorchidism. In this study, we investigate the possible ameliorative effect of curcumin (Cur) on the induced-unilateral cryptorchidism testicular injury in both cryptorchid (Cryp) and non-cryptorchid (non-Cryp) scrotal testes through histological, immunohistochemical and morphometrics.
Materials and methods: Forty adult male albino rats were divided into: control group, Cur control group, Cryp group, and Cryp+Cur group. The rat model was surgically established by fixing the left testis in the abdomen. The treated groups were subjected to surgically induced-unilateral cryptorchidism on the left side then were given Cur (80 mg/kg) orally, for 20 days. Histological analysis using haematoxylin and eosin and periodic acid Schiff’s reaction was done. Immunohistochemistry was performed for proliferating cell nuclear antigen (PCNA); to estimate the proliferation in the germinal epithelium, and vimentin; to evaluate Sertoli cells. The results were confirmed by statistical evaluation of the spermatogenic epithelium height, the seminiferous tubules diameter, the basement membrane thickness, the number of PCNA immunostained cells and the area per cent of vimentin immunostaining.
Results: Distorted seminiferous tubules, substantial degeneration of the germinal epithelium, thickening of the basement membrane with a significant decrease in PCNA and vimentin immunostaining were observed in Cryp group; mainly in the cryptorchid testis. These structural changes were significantly reversed in Crypt+Cur group.
Conclusions: Curcumin proved to be an important and effective medical line for protecting against the unfavourable sequels of cryptorchidism in a rat model.

Abstract

Background: Cryptorchidism, one or both hidden testes, is the most common abnormality of male sexual development. Subfertility or infertility is associated with both unilateral and bilateral cryptorchidism. In this study, we investigate the possible ameliorative effect of curcumin (Cur) on the induced-unilateral cryptorchidism testicular injury in both cryptorchid (Cryp) and non-cryptorchid (non-Cryp) scrotal testes through histological, immunohistochemical and morphometrics.
Materials and methods: Forty adult male albino rats were divided into: control group, Cur control group, Cryp group, and Cryp+Cur group. The rat model was surgically established by fixing the left testis in the abdomen. The treated groups were subjected to surgically induced-unilateral cryptorchidism on the left side then were given Cur (80 mg/kg) orally, for 20 days. Histological analysis using haematoxylin and eosin and periodic acid Schiff’s reaction was done. Immunohistochemistry was performed for proliferating cell nuclear antigen (PCNA); to estimate the proliferation in the germinal epithelium, and vimentin; to evaluate Sertoli cells. The results were confirmed by statistical evaluation of the spermatogenic epithelium height, the seminiferous tubules diameter, the basement membrane thickness, the number of PCNA immunostained cells and the area per cent of vimentin immunostaining.
Results: Distorted seminiferous tubules, substantial degeneration of the germinal epithelium, thickening of the basement membrane with a significant decrease in PCNA and vimentin immunostaining were observed in Cryp group; mainly in the cryptorchid testis. These structural changes were significantly reversed in Crypt+Cur group.
Conclusions: Curcumin proved to be an important and effective medical line for protecting against the unfavourable sequels of cryptorchidism in a rat model.

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Keywords

testis, unilateral cryptorchidism, curcumin, proliferating cell nuclear antigen (PCNA), vimentin

About this article
Title

The ameliorative effect of curcumin on cryptorchid and non-cryptorchid testes in induced unilateral cryptorchidism in albino rat: histological evaluation

Journal

Folia Morphologica

Issue

Vol 80, No 3 (2021)

Article type

Original article

Pages

596-604

Published online

2020-07-29

Page views

7012

Article views/downloads

1117

DOI

10.5603/FM.a2020.0084

Pubmed

32748946

Bibliographic record

Folia Morphol 2021;80(3):596-604.

Keywords

testis
unilateral cryptorchidism
curcumin
proliferating cell nuclear antigen (PCNA)
vimentin

Authors

M. A. Abd-El-Hafez
M. D. El-Shafee
S. H. Omar
A. A. Aburahma
S. S. Kamar

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