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Ahead of Print
ORIGINAL ARTICLES
Published online: 2020-03-11
Submitted: 2019-05-07
Accepted: 2019-11-30
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Histomorphometrical evaluation of tibial subchondral bone after moderate running in male and female Wistar rats

M. N. Nazem, M. Sahebozamani, R. Mosavian Naeini, S. M. Sajjadian
DOI: 10.5603/FM.a2020.0030
·
Pubmed: 32159840

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2020-03-11
Submitted: 2019-05-07
Accepted: 2019-11-30

Abstract

Exercise has been shown to be beneficial to the skeleton, in both humans and animals. This study was done to test the sex-related difference in the risk of OA (osteoarthritis) of the knee joint and also on the subchondral bone after moderate running exercise.

Forty male and female Wistar rats were randomly assigned to four equal groups (2 male and 2 female groups) in a same condition. Ten of each sex were selected as control groups, while running exercises were performed in remained 20 male and female rats using a motor treadmill to motivate rats to run daily distances of 1 km at 5 days/week within six weeks.

On day 43, all animals were sacrificed and the knee articular cartilage and also histomorphometric parameters of subchondral bone and mid shaft of tibia were evaluated.

Results showed mild OA in both male and female runner groups. Results in male runner rats were significantly lesser than that in female runners. On the other hand, the difference in female runner group showed significant changes in comparison with other groups in histomorphometric parameters in tibia.

Obtained results showed that the development of knee OA and subchondral bone changes may be related to the sex differences. Although there was no synovitis in male runners but female runner group showed mild hyperemia dropsy with a moderate synovitis in this region.

Abstract

Exercise has been shown to be beneficial to the skeleton, in both humans and animals. This study was done to test the sex-related difference in the risk of OA (osteoarthritis) of the knee joint and also on the subchondral bone after moderate running exercise.

Forty male and female Wistar rats were randomly assigned to four equal groups (2 male and 2 female groups) in a same condition. Ten of each sex were selected as control groups, while running exercises were performed in remained 20 male and female rats using a motor treadmill to motivate rats to run daily distances of 1 km at 5 days/week within six weeks.

On day 43, all animals were sacrificed and the knee articular cartilage and also histomorphometric parameters of subchondral bone and mid shaft of tibia were evaluated.

Results showed mild OA in both male and female runner groups. Results in male runner rats were significantly lesser than that in female runners. On the other hand, the difference in female runner group showed significant changes in comparison with other groups in histomorphometric parameters in tibia.

Obtained results showed that the development of knee OA and subchondral bone changes may be related to the sex differences. Although there was no synovitis in male runners but female runner group showed mild hyperemia dropsy with a moderate synovitis in this region.

Get Citation

Keywords

histomorphometry, running, subchondral bone, tibia, rat

About this article
Title

Histomorphometrical evaluation of tibial subchondral bone after moderate running in male and female Wistar rats

Journal

Folia Morphologica

Issue

Ahead of Print

Published online

2020-03-11

DOI

10.5603/FM.a2020.0030

Pubmed

32159840

Keywords

histomorphometry
running
subchondral bone
tibia
rat

Authors

M. N. Nazem
M. Sahebozamani
R. Mosavian Naeini
S. M. Sajjadian

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