Vol 77, No 3 (2018)
Original article
Published online: 2017-11-27
Endothelial expression of c-kit and CD68 in dental follicles of human impacted third molars
Abstract
Background: Periodontal tissue remnants of odontogenesis constitute the dental follicle (DF) which is actually considered a stem niche in adults. However, potentialities of local endothelia within this niche seem overlooked. We thus aimed at testing the endothelial cells expression of c-kit, the progenitor cells marker, and CD68, commonly regarded as a monocyte/macrophage marker, in human DFs.
Materials and methods: We performed an immunohistochemical study using these two markers which were applied on samples collected from ten adult patients.
Results: The markers were positively expressed in endothelial cells, as well as in spindle-shaped stromal cells of the DF.
Conclusions: The origin of DF stem or progenitor cells needs reviewing in the light of these findings, as endothelium could be a donor site for niche inhabitants.
Keywords: stem nichedental stem cellsmonocytesCD117immunohistochemistry
References
- Broudy VC, Kovach NL, Bennett LG, et al. Human umbilical vein endothelial cells display high-affinity c-kit receptors and produce a soluble form of the c-kit receptor. Blood. 1994; 83(8): 2145–2152.
- Carlile MJ, Sturrock MG, Chisholm DM, et al. The presence of pericytes and transitional cells in the vasculature of the human dental pulp: an ultrastructural study. Histochem J. 2000; 32(4): 239–245.
- Didilescu AC, Pop F, Rusu MC. c-kit positive cells and networks in tooth germs of human midterm fetuses. Ann Anat. 2013; 195(6): 581–585.
- Ding L, Saunders TL, Enikolopov G, et al. Endothelial and perivascular cells maintain haematopoietic stem cells. Nature. 2012; 481(7382): 457–462.
- Dziubińska P, Jaskólska M, Przyborowska P, et al. Stem cells in dentistry: review of literature. Pol J Vet Sci. 2013; 16(1): 135–140.
- Fitzgerald M, Chiego DJ, Heys DR. Autoradiographic analysis of odontoblast replacement following pulp exposure in primate teeth. Arch Oral Biol. 1990; 35(9): 707–715.
- Gottfried E, Kunz-Schughart LA, Weber A, et al. Expression of CD68 in non-myeloid cell types. Scand J Immunol. 2008; 67(5): 453–463.
- Huang GTJ, Gronthos S, Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res. 2009; 88(9): 792–806.
- Kémoun P, Laurencin-Dalicieux S, Rue J, et al. Localization of STRO-1, BMP-2/-3/-7, BMP receptors and phosphorylated Smad-1 during the formation of mouse periodontium. Tissue Cell. 2007; 39(4): 257–266.
- Kissa K, Herbomel P. Blood stem cells emerge from aortic endothelium by a novel type of cell transition. Nature. 2010; 464(7285): 112–115.
- La Rocca G, Anzalone R, Farina F. The expression of CD68 in human umbilical cord mesenchymal stem cells: new evidences of presence in non-myeloid cell types. Scand J Immunol. 2009; 70(2): 161–162.
- Laine M, Ventä I, Hyrkäs T, et al. Chronic inflammation around painless partially erupted third molars. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 95(3): 277–282.
- Lin Y, Weisdorf DJ, Solovey A, et al. Origins of circulating endothelial cells and endothelial outgrowth from blood. J Clin Invest. 2000; 105(1): 71–77.
- Majno G. Ultrastructure of the vascular membrane. Handbook of physiology. 1965; 3: 2293–2375.
- Ning H, Lin G, Lue TF, et al. Mesenchymal stem cell marker Stro-1 is a 75 kd endothelial antigen. Biochem Biophys Res Commun. 2011; 413(2): 353–357.
- Perlea P, Rusu MC, Didilescu AC, et al. Phenotype heterogeneity in dental pulp stem niches. Rom J Morphol Embryol. 2016; 57(4): 1187–1193.
- Petre N, Rusu MC, Pop F, et al. Telocytes of the mammary gland stroma. Folia Morphol. 2016; 75(2): 224–231.
- Petrovic V, Stefanovic V. Dental tissue--new source for stem cells. Scientific World J. 2009; 9: 1167–1177.
- Popescu LM, Faussone-Pellegrini MS. Telocytes - a case of serendipity: the winding way from Interstitial Cells of Cajal (ICC), via Interstitial Cajal-Like Cells (ICLC) to telocytes . J Cell Mol Med. 2010; 14(4): 729–740.
- Rusu MC, Hostiuc S, Dermengiu D, et al. STRO-1 positive pulmonary valve stem cells: preliminary report. Rom J Leg Med. 2015; 23(1): 1–4.
- Rusu MC, Hostiuc S, Vrapciu AD, et al. Subsets of telocytes: myocardial telocytes. Ann Anat. 2017; 209: 37–44.
- Saito MT, Silvério KG, Casati MZ, et al. Tooth-derived stem cells: Update and perspectives. World J Stem Cells. 2015; 7(2): 399–407.
- Schatteman GC, Awad O. Hemangioblasts, angioblasts, and adult endothelial cell progenitors. Anat Rec A Discov Mol Cell Evol Biol. 2004; 276(1): 13–21.
- Seta N, Kuwana M. Human circulating monocytes as multipotential progenitors. Keio J Med. 2007; 56(2): 41–47.
- Tambuwala AA, Oswal RG, Desale RS, et al. An evaluation of pathologic changes in the follicle of impacted mandibular third molars. J Int Oral Health. 2015; 7(4): 58–62.
- Zhang C, Cheng Xr. [Isolation and characterization of dental follicle cells from adult human dental follicle tissues]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2013; 48(2): 96–101.
- Zovein AC, Hofmann JJ, Lynch M, et al. Fate tracing reveals the endothelial origin of hematopoietic stem cells. Cell Stem Cell. 2008; 3(6): 625–636.
