open access

Vol 77, No 3 (2018)
ORIGINAL ARTICLES
Published online: 2017-11-27
Submitted: 2017-07-18
Accepted: 2017-11-10
Get Citation

Endothelial expression of c-kit and CD68 in dental follicles of human impacted third molars

M. C. Rusu, A. Rașcu, M. D. Stoenescu
DOI: 10.5603/FM.a2017.0108
·
Pubmed: 29235086
·
Folia Morphol 2018;77(3):485-488.

open access

Vol 77, No 3 (2018)
ORIGINAL ARTICLES
Published online: 2017-11-27
Submitted: 2017-07-18
Accepted: 2017-11-10

Abstract

Background: Periodontal tissue remnants of odontogenesis constitute the dental follicle (DF) which is actually considered a stem niche in adults. However, potentialities of local endothelia within this niche seem overlooked. We thus aimed at testing the endothelial cells expression of c-kit, the progenitor cells marker, and CD68, commonly regarded as a monocyte/macrophage marker, in human DFs.
Materials and methods: We performed an immunohistochemical study using these two markers which were applied on samples collected from ten adult patients.
Results: The markers were positively expressed in endothelial cells, as well as in spindle-shaped stromal cells of the DF.
Conclusions: The origin of DF stem or progenitor cells needs reviewing in the light of these findings, as endothelium could be a donor site for niche inhabitants.

Abstract

Background: Periodontal tissue remnants of odontogenesis constitute the dental follicle (DF) which is actually considered a stem niche in adults. However, potentialities of local endothelia within this niche seem overlooked. We thus aimed at testing the endothelial cells expression of c-kit, the progenitor cells marker, and CD68, commonly regarded as a monocyte/macrophage marker, in human DFs.
Materials and methods: We performed an immunohistochemical study using these two markers which were applied on samples collected from ten adult patients.
Results: The markers were positively expressed in endothelial cells, as well as in spindle-shaped stromal cells of the DF.
Conclusions: The origin of DF stem or progenitor cells needs reviewing in the light of these findings, as endothelium could be a donor site for niche inhabitants.

Get Citation

Keywords

stem niche; dental stem cells; monocytes; CD117; immunohistochemistry

About this article
Title

Endothelial expression of c-kit and CD68 in dental follicles of human impacted third molars

Journal

Folia Morphologica

Issue

Vol 77, No 3 (2018)

Pages

485-488

Published online

2017-11-27

DOI

10.5603/FM.a2017.0108

Pubmed

29235086

Bibliographic record

Folia Morphol 2018;77(3):485-488.

Keywords

stem niche
dental stem cells
monocytes
CD117
immunohistochemistry

Authors

M. C. Rusu
A. Rașcu
M. D. Stoenescu

References (27)
  1. Broudy VC, Kovach NL, Bennett LG, et al. Human umbilical vein endothelial cells display high-affinity c-kit receptors and produce a soluble form of the c-kit receptor. Blood. 1994; 83(8): 2145–2152.
  2. Carlile MJ, Sturrock MG, Chisholm DM, et al. The presence of pericytes and transitional cells in the vasculature of the human dental pulp: an ultrastructural study. Histochem J. 2000; 32(4): 239–245.
  3. Didilescu AC, Pop F, Rusu MC. c-kit positive cells and networks in tooth germs of human midterm fetuses. Ann Anat. 2013; 195(6): 581–585.
  4. Ding L, Saunders TL, Enikolopov G, et al. Endothelial and perivascular cells maintain haematopoietic stem cells. Nature. 2012; 481(7382): 457–462.
  5. Dziubińska P, Jaskólska M, Przyborowska P, et al. Stem cells in dentistry: review of literature. Pol J Vet Sci. 2013; 16(1): 135–140.
  6. Fitzgerald M, Chiego DJ, Heys DR. Autoradiographic analysis of odontoblast replacement following pulp exposure in primate teeth. Arch Oral Biol. 1990; 35(9): 707–715.
  7. Gottfried E, Kunz-Schughart LA, Weber A, et al. Expression of CD68 in non-myeloid cell types. Scand J Immunol. 2008; 67(5): 453–463.
  8. Huang GTJ, Gronthos S, Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res. 2009; 88(9): 792–806.
  9. Kémoun P, Laurencin-Dalicieux S, Rue J, et al. Localization of STRO-1, BMP-2/-3/-7, BMP receptors and phosphorylated Smad-1 during the formation of mouse periodontium. Tissue Cell. 2007; 39(4): 257–266.
  10. Kissa K, Herbomel P. Blood stem cells emerge from aortic endothelium by a novel type of cell transition. Nature. 2010; 464(7285): 112–115.
  11. La Rocca G, Anzalone R, Farina F. The expression of CD68 in human umbilical cord mesenchymal stem cells: new evidences of presence in non-myeloid cell types. Scand J Immunol. 2009; 70(2): 161–162.
  12. Laine M, Ventä I, Hyrkäs T, et al. Chronic inflammation around painless partially erupted third molars. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 95(3): 277–282.
  13. Lin Y, Weisdorf DJ, Solovey A, et al. Origins of circulating endothelial cells and endothelial outgrowth from blood. J Clin Invest. 2000; 105(1): 71–77.
  14. Majno G. Ultrastructure of the vascular membrane. Handbook of physiology. 1965; 3: 2293–2375.
  15. Ning H, Lin G, Lue TF, et al. Mesenchymal stem cell marker Stro-1 is a 75 kd endothelial antigen. Biochem Biophys Res Commun. 2011; 413(2): 353–357.
  16. Perlea P, Rusu MC, Didilescu AC, et al. Phenotype heterogeneity in dental pulp stem niches. Rom J Morphol Embryol. 2016; 57(4): 1187–1193.
  17. Petre N, Rusu MC, Pop F, et al. Telocytes of the mammary gland stroma. Folia Morphol. 2016; 75(2): 224–231.
  18. Petrovic V, Stefanovic V. Dental tissue--new source for stem cells. Scientific World J. 2009; 9: 1167–1177.
  19. Popescu LM, Faussone-Pellegrini MS. Telocytes - a case of serendipity: the winding way from Interstitial Cells of Cajal (ICC), via Interstitial Cajal-Like Cells (ICLC) to telocytes . J Cell Mol Med. 2010; 14(4): 729–740.
  20. Rusu MC, Hostiuc S, Dermengiu D, et al. STRO-1 positive pulmonary valve stem cells: preliminary report. Rom J Leg Med. 2015; 23(1): 1–4.
  21. Rusu MC, Hostiuc S, Vrapciu AD, et al. Subsets of telocytes: myocardial telocytes. Ann Anat. 2017; 209: 37–44.
  22. Saito MT, Silvério KG, Casati MZ, et al. Tooth-derived stem cells: Update and perspectives. World J Stem Cells. 2015; 7(2): 399–407.
  23. Schatteman GC, Awad O. Hemangioblasts, angioblasts, and adult endothelial cell progenitors. Anat Rec A Discov Mol Cell Evol Biol. 2004; 276(1): 13–21.
  24. Seta N, Kuwana M. Human circulating monocytes as multipotential progenitors. Keio J Med. 2007; 56(2): 41–47.
  25. Tambuwala AA, Oswal RG, Desale RS, et al. An evaluation of pathologic changes in the follicle of impacted mandibular third molars. J Int Oral Health. 2015; 7(4): 58–62.
  26. Zhang C, Cheng Xr. [Isolation and characterization of dental follicle cells from adult human dental follicle tissues]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2013; 48(2): 96–101.
  27. Zovein AC, Hofmann JJ, Lynch M, et al. Fate tracing reveals the endothelial origin of hematopoietic stem cells. Cell Stem Cell. 2008; 3(6): 625–636.

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl