open access

Vol 77, No 2 (2018)
ORIGINAL ARTICLES
Published online: 2017-11-06
Submitted: 2017-04-26
Accepted: 2017-10-05
Get Citation

Immunohistochemical study on roe deer haemal nodes

Y. Akaydın Bozkurt, E. Karadağ Sarı, M. Kabak
DOI: 10.5603/FM.a2017.0103
·
Pubmed: 29131281
·
Folia Morphol 2018;77(2):266-271.

open access

Vol 77, No 2 (2018)
ORIGINAL ARTICLES
Published online: 2017-11-06
Submitted: 2017-04-26
Accepted: 2017-10-05

Abstract

Background: The aim of this study was to investigate the immunohistochemical characteristics of the haemal nodes located in the abdominal and thoracic cavities in roe deer (Capreolus capreolus).

Materials and methods: In this study, 2 adult male and 2 adult female roe deers in addition to 2 roe deer foetuses at the late foetal stages were used. The avidin-biotin-peroxidase technique was applied to anti-CD3, anti-CD79acy, anti­-macrophage, anti-S100 primary antibodies.

Results: Positive results were gained for all the antibodies used. Many CD3 positive T-lymphocytes were seen in the lymphoid tissue areas of the foetal haemal nodes, in the germinal centre of the lymph follicles of the adult haemal nodes and in their lymphatic cords. CD79acy positive cells were less in number while S-100 protein was positive in both lymph follicles and in sinuses of the adult haemal nodes. In foetal haemal nodes, cells positive for S-100 protein were more prevalent in the sinuses. For the MAC387 primary antibody, there were positive macrophages seen in the capsule and trabeculae of the haemal nodes, located denser in males. Macrophage cells in the lymphoid structures and granulocytes in the sinuses of the haemal nodes of both adults and foetuses were positive for MAC387.

Conclusions: This study is the first one to define the detailed structural features immunohistochemically in the haemal nodes of the roe deer. Even it’s concluded that the results were similar with the other ruminants, it’s also pointed out that there are certain differences among the foetal, adult male and female haemal nodes of the roe deer. (Folia Morphol 2018; 77, 2: 266–271)

Abstract

Background: The aim of this study was to investigate the immunohistochemical characteristics of the haemal nodes located in the abdominal and thoracic cavities in roe deer (Capreolus capreolus).

Materials and methods: In this study, 2 adult male and 2 adult female roe deers in addition to 2 roe deer foetuses at the late foetal stages were used. The avidin-biotin-peroxidase technique was applied to anti-CD3, anti-CD79acy, anti­-macrophage, anti-S100 primary antibodies.

Results: Positive results were gained for all the antibodies used. Many CD3 positive T-lymphocytes were seen in the lymphoid tissue areas of the foetal haemal nodes, in the germinal centre of the lymph follicles of the adult haemal nodes and in their lymphatic cords. CD79acy positive cells were less in number while S-100 protein was positive in both lymph follicles and in sinuses of the adult haemal nodes. In foetal haemal nodes, cells positive for S-100 protein were more prevalent in the sinuses. For the MAC387 primary antibody, there were positive macrophages seen in the capsule and trabeculae of the haemal nodes, located denser in males. Macrophage cells in the lymphoid structures and granulocytes in the sinuses of the haemal nodes of both adults and foetuses were positive for MAC387.

Conclusions: This study is the first one to define the detailed structural features immunohistochemically in the haemal nodes of the roe deer. Even it’s concluded that the results were similar with the other ruminants, it’s also pointed out that there are certain differences among the foetal, adult male and female haemal nodes of the roe deer. (Folia Morphol 2018; 77, 2: 266–271)

Get Citation

Keywords

CD3, CD79acy, macrophage, S-100 protein, immunohistochemistry, haemal node, roe deer

About this article
Title

Immunohistochemical study on roe deer haemal nodes

Journal

Folia Morphologica

Issue

Vol 77, No 2 (2018)

Pages

266-271

Published online

2017-11-06

DOI

10.5603/FM.a2017.0103

Pubmed

29131281

Bibliographic record

Folia Morphol 2018;77(2):266-271.

Keywords

CD3
CD79acy
macrophage
S-100 protein
immunohistochemistry
haemal node
roe deer

Authors

Y. Akaydın Bozkurt
E. Karadağ Sarı
M. Kabak

References (19)
  1. Akaydin Bozkurt Y, Kabak M. Morphology of haemal nodes in the roe deer (Capreolus capreolus). Anat Histol Embryol. 2010; 39(5): 456–461.
  2. Bacha WJ, Bacha LM. Color Atlas of Veterinary Histology. Third ed. Wiley-Blackwell, A John Wiley&Sons, Inc, Publication, UK. 2012.
  3. Casteleyn CR, Breugelmans S, Simoens P, et al. Morphological and immunological characteristics of the bovine temporal lymph node and hemal node. Vet Immunol Immunopathol. 2008; 126(3-4): 339–350.
  4. Ceccarelli P, Gargiulo AM, Fagioli O, et al. Cytochemical identification of lymphocytes and other mononuclear cells in ovine and bovine hemal nodes. Comp Immunol Microbiol Infect Dis. 1986; 9(4): 297–302.
  5. Cerutti P, Marcaccini A, Guerrero F. A Scanning and Immunohistochemical Study in Bovine Haemal Node. Anat Histol Embryol. 1998; 27(6): 387–392.
  6. Cocchia D, Tiberio G, Santarelli R, et al. S-100 protein in "follicular dendritic" cells or rat lymphoid organs. An immunochemical and immunocytochemical study. Cell Tissue Res. 1983; 230(1): 95–103.
  7. Dassanayake RP, Truscott TC, Özyiğit MÖ, et al. Accumulation profiles of PrP(Sc) in hemal nodes of naturally and experimentally scrapie-infected sheep. BMC Vet Res. 2013; 9: 82.
  8. Ezeasor DN, Singh A, Sims DE, et al. Histology of the caprine hemal node. Acta Anat (Basel). 1988; 133(1): 16–23.
  9. Eurell JA, Frappier BL. Dellmann Textbook of Veterinary Histology. Sixth ed. Blackwell Publishing, USA. 2006.
  10. Frandson RD, Wilke WL, Fails AD. Anatomy and Physiology of Farm Animals. Seventh ed. Wiley-Blackwell, A John Wiley&Sons, Inc, Publication, USA. 2009.
  11. Guerrero F, Cerutti P, Marcaccini A, et al. Histological and immunohistochemical study on Iberian red deer (Cervus elaphus hispanicus) haemal nodes. Microsc Microanal. 2012; 18(Suppl 5): 1–2.
  12. Ozaydin T, Sur E, Celik I, et al. Histological and enzyme histochemical investigation of the hemal nodes of the hair goat. Biotech Histochem. 2012; 87(6): 377–384.
  13. Romero-Palomo F, Risalde MA, Molina V, et al. Immunohistochemical detection of dendritic cell markers in cattle. Vet Pathol. 2013; 50(6): 1099–1108.
  14. Sugimura M, Shirogane D, Atoji Y, et al. A comparative study on S-100 protein-immunoreactive cells in lymph nodes. Nihon Juigaku Zasshi. 1990; 52(5): 1015–1021.
  15. Sur E, Aydın MF, Çelik İA. Light microscopic investigation on the histology and Alpha-Naphthyl Acetate Esterase (ANAE)-positive lymphocyte localization in the hemal nodes of Akkaraman sheep. Eurasian J Vet Sci. 2005; 21(1-2): 101–108.
  16. Thorp BH, Seneque S, Staute K, et al. Characterization and distribution of lymphocyte subsets in sheep hemal nodes. Dev Comp Immunol. 1991; 15(4): 393–400.
  17. Windqvist G. The bovine hemal nodes. Acta Anat. 1954; 22: 108–112.
  18. Yoon, YS, Lee JS, Lee HS, Kim JS. Morphological studies on the hemal node and hemolymph node in the Korean native goat. Korean J Anat. 1989; 22: 261–278.
  19. Zidan M, Pabst R. Histological, histochemical and immunohistochemical study of the haemal nodes of the dromedary camel. Anat Histol Embryol. 2004; 33(5): 284–289.

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl