open access

Vol 76, No 4 (2017)
ORIGINAL ARTICLES
Published online: 2017-04-19
Submitted: 2017-02-17
Accepted: 2017-04-03
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The possible protective effect of L-arginine against 5-fluorouracil-induced nephrotoxicity in male albino rats

M. H. Badawoud, E. B. Elshal, A. I. Zaki, H. A. Amin
DOI: 10.5603/FM.a2017.0037
·
Pubmed: 28553862
·
Folia Morphol 2017;76(4):608-619.

open access

Vol 76, No 4 (2017)
ORIGINAL ARTICLES
Published online: 2017-04-19
Submitted: 2017-02-17
Accepted: 2017-04-03

Abstract

5-fluorouracil (5-FU) is a potent antineoplastic agent used for the treatment of various malignancies. The L-arginine nitric oxide (NO) pathway involved in the pathogenesis of chemotherapy induced kidney damage. This work investigated the beneficial mechanism of L-arginine supplementation in 5-FU induced nephropathy. Eighty male Wistar rats were divided into four equal groups: control group; L-arginine group (378 mg/rat/day for 4 weeks); 5-FU group (189 mg/rat/week for 4 weeks) and L-arginine for 1 week before and 4 weeks concomitant with 5-FU group. At the end of experiment, the kidney functions were assessed and kidneys specimens were processed for paraffin sections and stained with haematoxylin and eosin (H&E), Masson’s trichome (MT) and periodic acid-Schiff (PAS) stains. Other sections were processed for immunohistochemical demonstration of caspase-3 and inducible NO synthase (iNOS). Image analyser was used to analyse the results morphometrically and statistically. L-arginine administration to 5-FU treated animals elicited significant reduction in serum urea and creatinine levels, urine volume, urinary protein excretion and kidney/body weight ratio in comparison to fluorouracil treated group. L-arginine improved glomeruloscelerosis, degeneration of convoluted tubules and interstitial fibrosis in 5-FU treated animals. L-arginine attenuated effectively some biochemical and histological changes in 5-FU nephrotoxicity.

Abstract

5-fluorouracil (5-FU) is a potent antineoplastic agent used for the treatment of various malignancies. The L-arginine nitric oxide (NO) pathway involved in the pathogenesis of chemotherapy induced kidney damage. This work investigated the beneficial mechanism of L-arginine supplementation in 5-FU induced nephropathy. Eighty male Wistar rats were divided into four equal groups: control group; L-arginine group (378 mg/rat/day for 4 weeks); 5-FU group (189 mg/rat/week for 4 weeks) and L-arginine for 1 week before and 4 weeks concomitant with 5-FU group. At the end of experiment, the kidney functions were assessed and kidneys specimens were processed for paraffin sections and stained with haematoxylin and eosin (H&E), Masson’s trichome (MT) and periodic acid-Schiff (PAS) stains. Other sections were processed for immunohistochemical demonstration of caspase-3 and inducible NO synthase (iNOS). Image analyser was used to analyse the results morphometrically and statistically. L-arginine administration to 5-FU treated animals elicited significant reduction in serum urea and creatinine levels, urine volume, urinary protein excretion and kidney/body weight ratio in comparison to fluorouracil treated group. L-arginine improved glomeruloscelerosis, degeneration of convoluted tubules and interstitial fibrosis in 5-FU treated animals. L-arginine attenuated effectively some biochemical and histological changes in 5-FU nephrotoxicity.

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Keywords

L-arginine, nephrotoxicity, 5-fluorouracil

About this article
Title

The possible protective effect of L-arginine against 5-fluorouracil-induced nephrotoxicity in male albino rats

Journal

Folia Morphologica

Issue

Vol 76, No 4 (2017)

Pages

608-619

Published online

2017-04-19

DOI

10.5603/FM.a2017.0037

Pubmed

28553862

Bibliographic record

Folia Morphol 2017;76(4):608-619.

Keywords

L-arginine
nephrotoxicity
5-fluorouracil

Authors

M. H. Badawoud
E. B. Elshal
A. I. Zaki
H. A. Amin

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