open access

Vol 75, No 3 (2016)
ORIGINAL ARTICLES
Published online: 2016-02-24
Submitted: 2015-12-17
Accepted: 2016-02-05
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Macro- and microstructural descriptions of the zebrafish (Danio rerio) liver

L. B. de Oliveira, A. S. Leonardo, E. M. M. de Lima
DOI: 10.5603/FM.a2016.0009
·
Pubmed: 26916203
·
Folia Morphol 2016;75(3):382-387.

open access

Vol 75, No 3 (2016)
ORIGINAL ARTICLES
Published online: 2016-02-24
Submitted: 2015-12-17
Accepted: 2016-02-05

Abstract

The liver is an essential metabolically active organ, responsible for many vital life functions such as metabolism, synthesis, and storage. This study describes the macroscopic and microscopic zebrafish liver and its temporal changes during a period of 13 weeks. Forty-six adult zebrafish were used (46), of which 27 were females and were 19 males. Seven fish were euthanized initially for macroscopic evaluation and 3 fish were euthanized weekly for 13 weeks for microscopic evaluation. Routine histological technique was performed; the slides were stained with haematoxylin/eosin and periodic acid-Schiff. The volumetric (Vv(h)) and numerical (Nv(h)) densities of hepatocytes and vacuolation intensity were determined weekly. The liver was located in the coelomic cavity ventrally to the swim bladder, slightly brownish and divided into 3 hepatic lobes. Microscopically, it consisted of hepatocytes forming a muralium duplex around the sinusoids. The staining of the cytoplasm of hepatocytes was more eosinophilic and more basophilic in males and females, respectively. There was no formation of portal triads and phagocytic cells. The gallbladder had transitional epithelium, and the bile ducts had simple cuboidal epithelium. The volume and numerical density of hepatocytes during the 13 weeks was not statistically different, but these parameters displayed a positive correlation. The vacuolation was due to the presence of glycogen. The zebrafish liver has macro and micro structural particular features while, quantitatively, the volume and number of hepatocytes showed great capacity to respond to the physiological needs of the animal at the time.

Abstract

The liver is an essential metabolically active organ, responsible for many vital life functions such as metabolism, synthesis, and storage. This study describes the macroscopic and microscopic zebrafish liver and its temporal changes during a period of 13 weeks. Forty-six adult zebrafish were used (46), of which 27 were females and were 19 males. Seven fish were euthanized initially for macroscopic evaluation and 3 fish were euthanized weekly for 13 weeks for microscopic evaluation. Routine histological technique was performed; the slides were stained with haematoxylin/eosin and periodic acid-Schiff. The volumetric (Vv(h)) and numerical (Nv(h)) densities of hepatocytes and vacuolation intensity were determined weekly. The liver was located in the coelomic cavity ventrally to the swim bladder, slightly brownish and divided into 3 hepatic lobes. Microscopically, it consisted of hepatocytes forming a muralium duplex around the sinusoids. The staining of the cytoplasm of hepatocytes was more eosinophilic and more basophilic in males and females, respectively. There was no formation of portal triads and phagocytic cells. The gallbladder had transitional epithelium, and the bile ducts had simple cuboidal epithelium. The volume and numerical density of hepatocytes during the 13 weeks was not statistically different, but these parameters displayed a positive correlation. The vacuolation was due to the presence of glycogen. The zebrafish liver has macro and micro structural particular features while, quantitatively, the volume and number of hepatocytes showed great capacity to respond to the physiological needs of the animal at the time.

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Keywords

hepatocytes, fish density, macroscopy, glycogen

About this article
Title

Macro- and microstructural descriptions of the zebrafish (Danio rerio) liver

Journal

Folia Morphologica

Issue

Vol 75, No 3 (2016)

Pages

382-387

Published online

2016-02-24

DOI

10.5603/FM.a2016.0009

Pubmed

26916203

Bibliographic record

Folia Morphol 2016;75(3):382-387.

Keywords

hepatocytes
fish density
macroscopy
glycogen

Authors

L. B. de Oliveira
A. S. Leonardo
E. M. M. de Lima

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