open access

Vol 75, No 2 (2016)
ORIGINAL ARTICLES
Published online: 2015-10-02
Submitted: 2015-06-14
Accepted: 2015-08-11
Get Citation

Morphological characteristics of the sinus node on postmortem tissue

F. V. Perde, A. Atkinson, J. Yanni, D. Dermengiu, H. Dobrzynski
DOI: 10.5603/FM.a2015.0087
·
Pubmed: 26431048
·
Folia Morphol 2016;75(2):216-223.

open access

Vol 75, No 2 (2016)
ORIGINAL ARTICLES
Published online: 2015-10-02
Submitted: 2015-06-14
Accepted: 2015-08-11

Abstract

The sinus node is an intensively researched structure in terms of anatomical, histological, electrophysiological, molecular and genetic approach. For postmortem diagnosis it is still difficult to investigate due to a still reduced accessibility. In this study we tried and succeed to apply molecular biology techniques on postmortem tissues in order to widen the range of postmortem forensic investigation and provide information related to the diagnostic of cardiac arrhythmia. We described the stages of this investigation, with dissection, preservation and analysis that included classical histology, immunohistochemistry, confocal microscope, microdissection, RIN testing, mRNA expression obtaining a precise morphofunctional location of the sinus node.

Abstract

The sinus node is an intensively researched structure in terms of anatomical, histological, electrophysiological, molecular and genetic approach. For postmortem diagnosis it is still difficult to investigate due to a still reduced accessibility. In this study we tried and succeed to apply molecular biology techniques on postmortem tissues in order to widen the range of postmortem forensic investigation and provide information related to the diagnostic of cardiac arrhythmia. We described the stages of this investigation, with dissection, preservation and analysis that included classical histology, immunohistochemistry, confocal microscope, microdissection, RIN testing, mRNA expression obtaining a precise morphofunctional location of the sinus node.

Get Citation

Keywords

messenger RNA, autopsy, connexin 43

About this article
Title

Morphological characteristics of the sinus node on postmortem tissue

Journal

Folia Morphologica

Issue

Vol 75, No 2 (2016)

Pages

216-223

Published online

2015-10-02

DOI

10.5603/FM.a2015.0087

Pubmed

26431048

Bibliographic record

Folia Morphol 2016;75(2):216-223.

Keywords

messenger RNA
autopsy
connexin 43

Authors

F. V. Perde
A. Atkinson
J. Yanni
D. Dermengiu
H. Dobrzynski

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