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Follicular dynamics in neonate vitrified ovarian grafts after host treatment with melatonin
open access
Abstract
The results showed that the sites of transplantation became obvious within the oestrous phase. Histological analysis showed that there was a dynamic of the ovogenesis process in the vitrified ovary grafts. However, in the ovary graft the empty cavity together with jumbled oocyte-granulosa complex in the non-treated group was higher than in the melatonin treated group.
Overall, the number of primary follicles in the ovary grafts of both groups remained constant throughout the oestrous period. However, the treated ovary grafts contained more secondary follicles throughout the oestrous period in comparison to non-treated ovary grafts. The antral follicle rates were more marked in the melatonin treated group than in the non-treated group during the oestrous period, whereas at other days of the cycle no significant difference was observed. The fresh corpora lutea rates were significantly higher in the treatment group than in the non-treatment group.
The study showed that there is a positive effect with melatonin resulting in more grafts restoring puberty. Furthermore, the associated increase in healthy follicles suggests that melatonin has a preventative ischaemia/antioxidant action and may be useful to follicles. (Folia Morphol 2011; 70, 1: 18-23)
Abstract
The results showed that the sites of transplantation became obvious within the oestrous phase. Histological analysis showed that there was a dynamic of the ovogenesis process in the vitrified ovary grafts. However, in the ovary graft the empty cavity together with jumbled oocyte-granulosa complex in the non-treated group was higher than in the melatonin treated group.
Overall, the number of primary follicles in the ovary grafts of both groups remained constant throughout the oestrous period. However, the treated ovary grafts contained more secondary follicles throughout the oestrous period in comparison to non-treated ovary grafts. The antral follicle rates were more marked in the melatonin treated group than in the non-treated group during the oestrous period, whereas at other days of the cycle no significant difference was observed. The fresh corpora lutea rates were significantly higher in the treatment group than in the non-treatment group.
The study showed that there is a positive effect with melatonin resulting in more grafts restoring puberty. Furthermore, the associated increase in healthy follicles suggests that melatonin has a preventative ischaemia/antioxidant action and may be useful to follicles. (Folia Morphol 2011; 70, 1: 18-23)
Keywords
melatonin; ovaries allograft; vitrification; reactive oxygen species; ovariectomy; oestrous cyclicity
Title
Follicular dynamics in neonate vitrified ovarian grafts after host treatment with melatonin
Journal
Issue
Article type
Original article
Pages
18-23
Published online
2011-03-03
Page views
598
Article views/downloads
1296
Bibliographic record
Folia Morphol 2011;70(1):18-23.
Keywords
melatonin
ovaries allograft
vitrification
reactive oxygen species
ovariectomy
oestrous cyclicity
Authors
M. Hemadi
G. Saki
S. Shokri
F.M. Ghasemi