Enhancement of the focal depth in anatomical photography

Abstract

Limited depth of field is one of the crucial disadvantages of macro photography because some details of the imagined object are blurred. This paper presents the benefits of using an algorithm which enhances focal depth in the close-up views of anatomical structures. The applied technique was based on combining a set of images of the same object (temporal bone) taken on different focal planes. In effect, a single image was generated which presented all details sharply across the photographed object. The extended depth of field of the composite image was reconstructed by CombineZP Image Stacking Software. (Folia Morphol 2011; 70, 4: 260–262)