open access

Vol 70, No 4 (2011)
ORIGINAL ARTICLES
Published online: 2011-11-25
Submitted: 2012-06-27
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Estimation of Purkinje cell quantification and volumetry in the cerebellum using a stereological technique

M.S. Akosman, N. Gocmen-Mas, H.S. Karabekir
Folia Morphol 2011;70(4):240-244.

open access

Vol 70, No 4 (2011)
ORIGINAL ARTICLES
Published online: 2011-11-25
Submitted: 2012-06-27

Abstract

Systematic sampling using the Cavalier’s principle provides accurate, efficient, cheap, and simple quantitative estimates of objects within neuroanatomical structures like the cerebellum. We identified and isolated the Purkinje cell layer and used this information to extrapolate the stereological technique to estimate the total number of Purkinje cells and volume on light microscope in rabbits’ cerebella. Volumes of the cell populations in the cerebellum have not been estimated previously. Using this method, we counted the Purkinje cells in the two right and three left hemicerebella of five male healthy rabbits and found the total number of Purkinje cells to have a mean of 671,597. The mean volume of the Purkinje cell was estimated at 2207 μm3 for the entire cerebella. The contribution of the stereological method to cell quantification and volumetry was emphasised by the neuroanatomical experimental animal study. The method proved to be an excellent tool for evaluating the Purkinje cell numbers and volumes in the rabbits. The data may also support the significance of considering anatomical data when evaluating pathological changes in cerebella. (Folia Morphol 2011; 70, 4: 240–244)

Abstract

Systematic sampling using the Cavalier’s principle provides accurate, efficient, cheap, and simple quantitative estimates of objects within neuroanatomical structures like the cerebellum. We identified and isolated the Purkinje cell layer and used this information to extrapolate the stereological technique to estimate the total number of Purkinje cells and volume on light microscope in rabbits’ cerebella. Volumes of the cell populations in the cerebellum have not been estimated previously. Using this method, we counted the Purkinje cells in the two right and three left hemicerebella of five male healthy rabbits and found the total number of Purkinje cells to have a mean of 671,597. The mean volume of the Purkinje cell was estimated at 2207 μm3 for the entire cerebella. The contribution of the stereological method to cell quantification and volumetry was emphasised by the neuroanatomical experimental animal study. The method proved to be an excellent tool for evaluating the Purkinje cell numbers and volumes in the rabbits. The data may also support the significance of considering anatomical data when evaluating pathological changes in cerebella. (Folia Morphol 2011; 70, 4: 240–244)
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Keywords

Purkinje cell number; volume; estimation; stereological method

About this article
Title

Estimation of Purkinje cell quantification and volumetry in the cerebellum using a stereological technique

Journal

Folia Morphologica

Issue

Vol 70, No 4 (2011)

Pages

240-244

Published online

2011-11-25

Bibliographic record

Folia Morphol 2011;70(4):240-244.

Keywords

Purkinje cell number
volume
estimation
stereological method

Authors

M.S. Akosman
N. Gocmen-Mas
H.S. Karabekir

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