Vol 70, No 4 (2011)
Original article
Submitted: 2012-06-27
Published online: 2011-11-25
Estimation of Purkinje cell quantification and volumetry in the cerebellum using a stereological technique
M.S. Akosman, N. Gocmen-Mas, H.S. Karabekir
Folia Morphol 2011;70(4):240-244.
Vol 70, No 4 (2011)
ORIGINAL ARTICLES
Submitted: 2012-06-27
Published online: 2011-11-25
Abstract
Systematic sampling using the Cavalier’s principle provides accurate, efficient,
cheap, and simple quantitative estimates of objects within neuroanatomical
structures like the cerebellum. We identified and isolated the Purkinje
cell layer and used this information to extrapolate the stereological technique
to estimate the total number of Purkinje cells and volume on light
microscope in rabbits’ cerebella. Volumes of the cell populations in the cerebellum
have not been estimated previously. Using this method, we counted
the Purkinje cells in the two right and three left hemicerebella of five
male healthy rabbits and found the total number of Purkinje cells to have
a mean of 671,597. The mean volume of the Purkinje cell was estimated at
2207 μm3 for the entire cerebella. The contribution of the stereological
method to cell quantification and volumetry was emphasised by the neuroanatomical
experimental animal study. The method proved to be an excellent
tool for evaluating the Purkinje cell numbers and volumes in the
rabbits. The data may also support the significance of considering anatomical
data when evaluating pathological changes in cerebella. (Folia Morphol
2011; 70, 4: 240–244)
Abstract
Systematic sampling using the Cavalier’s principle provides accurate, efficient,
cheap, and simple quantitative estimates of objects within neuroanatomical
structures like the cerebellum. We identified and isolated the Purkinje
cell layer and used this information to extrapolate the stereological technique
to estimate the total number of Purkinje cells and volume on light
microscope in rabbits’ cerebella. Volumes of the cell populations in the cerebellum
have not been estimated previously. Using this method, we counted
the Purkinje cells in the two right and three left hemicerebella of five
male healthy rabbits and found the total number of Purkinje cells to have
a mean of 671,597. The mean volume of the Purkinje cell was estimated at
2207 μm3 for the entire cerebella. The contribution of the stereological
method to cell quantification and volumetry was emphasised by the neuroanatomical
experimental animal study. The method proved to be an excellent
tool for evaluating the Purkinje cell numbers and volumes in the
rabbits. The data may also support the significance of considering anatomical
data when evaluating pathological changes in cerebella. (Folia Morphol
2011; 70, 4: 240–244)
Keywords
Purkinje cell number; volume; estimation; stereological method
Title
Estimation of Purkinje cell quantification and volumetry in the cerebellum using a stereological technique
Journal
Folia Morphologica
Issue
Vol 70, No 4 (2011)
Article type
Original article
Pages
240-244
Published online
2011-11-25
Page views
615
Article views/downloads
2703
Bibliographic record
Folia Morphol 2011;70(4):240-244.
Keywords
Purkinje cell number
volume
estimation
stereological method
Authors
M.S. Akosman
N. Gocmen-Mas
H.S. Karabekir