open access

Vol 71, No 2 (2012)
ORIGINAL ARTICLES
Published online: 2012-05-30
Submitted: 2012-06-27
Get Citation

Matrix change of bone grafting substitute after implantation into guinea pig bulla

Ch. Punke, T. Zehlicke, T. Just, G. Holzhüter, T. Gerber, H.W. Pau
Folia Morphol 2012;71(2):109-114.

open access

Vol 71, No 2 (2012)
ORIGINAL ARTICLES
Published online: 2012-05-30
Submitted: 2012-06-27

Abstract


Background: Many different surgical techniques have been developed to remove open mastoid cavities. In addition to autologous materials, alloplastic substances have been used. A very slow absorption of these materials and extrusion reactions have been reported. We investigated a newly developed, highly porous bone grafting material to eliminate open mastoid cavities, in an animal model. To characterise the transformation process, the early tissue reactions were studied in relation to the matrix transformation of the bone material.
Material and methods: NanoBone (NB), a highly porous bone grafting material based on calcium phosphate and silica, was filled into the open bullae from 20 guinea pigs. The bullae were examined histologically. Energy dispersive X-ray spectroscopy (EDX) was used to investigate the change in the elemental composition at different sampling times. The surface topography of the sections was examined by electron microscopy.
Results: After 1 week, periodic acid-Schiffs (PAS) staining demonstrated accumulation of glycogen and proteins, particularly in the border area of the NB particles. After 2 weeks, the particles were evenly coloured after PAS staining. EDX analysis showed a rapid absorption of the silica in the bone grafting material.
Conclusions: NanoBone showed a rapid matrix change after implantation in the bullae of guinea pigs. The absorption of the silica matrix and replacement by PAS-positive substances like glycoproteins and mucopolysaccharides seems to play a decisive role in the degradation processes of NB. This is associated with the good osteoinductive properties of the material.

Abstract


Background: Many different surgical techniques have been developed to remove open mastoid cavities. In addition to autologous materials, alloplastic substances have been used. A very slow absorption of these materials and extrusion reactions have been reported. We investigated a newly developed, highly porous bone grafting material to eliminate open mastoid cavities, in an animal model. To characterise the transformation process, the early tissue reactions were studied in relation to the matrix transformation of the bone material.
Material and methods: NanoBone (NB), a highly porous bone grafting material based on calcium phosphate and silica, was filled into the open bullae from 20 guinea pigs. The bullae were examined histologically. Energy dispersive X-ray spectroscopy (EDX) was used to investigate the change in the elemental composition at different sampling times. The surface topography of the sections was examined by electron microscopy.
Results: After 1 week, periodic acid-Schiffs (PAS) staining demonstrated accumulation of glycogen and proteins, particularly in the border area of the NB particles. After 2 weeks, the particles were evenly coloured after PAS staining. EDX analysis showed a rapid absorption of the silica in the bone grafting material.
Conclusions: NanoBone showed a rapid matrix change after implantation in the bullae of guinea pigs. The absorption of the silica matrix and replacement by PAS-positive substances like glycoproteins and mucopolysaccharides seems to play a decisive role in the degradation processes of NB. This is associated with the good osteoinductive properties of the material.
Get Citation

Keywords

obliterating open mastoid cavities; open mastoid cavity; highly porous bone augmentation materials; NanoBone; silica

About this article
Title

Matrix change of bone grafting substitute after implantation into guinea pig bulla

Journal

Folia Morphologica

Issue

Vol 71, No 2 (2012)

Pages

109-114

Published online

2012-05-30

Bibliographic record

Folia Morphol 2012;71(2):109-114.

Keywords

obliterating open mastoid cavities
open mastoid cavity
highly porous bone augmentation materials
NanoBone
silica

Authors

Ch. Punke
T. Zehlicke
T. Just
G. Holzhüter
T. Gerber
H.W. Pau

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl