open access

Vol 59, No 2 (2000)
ORIGINAL ARTICLES
Published online: 2000-02-16
Submitted: 2012-02-06
Get Citation

The effects of short-time caffeine administration on skeleton development in Wistar rats

Franciszek Burdan, Barbara Medej, Zbigniew Wójtowicz, Ryszard Maciejewski
Folia Morphol 2000;59(2):91-95.

open access

Vol 59, No 2 (2000)
ORIGINAL ARTICLES
Published online: 2000-02-16
Submitted: 2012-02-06

Abstract

The aim of the study was to evaluate the influence of caffeine on skeleton ossification in rats. Caffeine was administered in Tween 80 solution, once daily, in oral bolus, during the whole second trimester, in three doses: C1 — 0.7 mg/kg, C2 — 7.0 mg/kg, C3 — 70.0 mg/kg. On the 21st day of gestation the pumps were delivered. The fetuses were fixed in Bouin’s solution and subsequently observed for external and internal malformation or in alcohol for skeleton malformation. The skeletons were stained with alizarin red-S. The examination showed an insignificant (P < 0.05) number of skeleton malformations, external haematomas and any internal malformations.

Abstract

The aim of the study was to evaluate the influence of caffeine on skeleton ossification in rats. Caffeine was administered in Tween 80 solution, once daily, in oral bolus, during the whole second trimester, in three doses: C1 — 0.7 mg/kg, C2 — 7.0 mg/kg, C3 — 70.0 mg/kg. On the 21st day of gestation the pumps were delivered. The fetuses were fixed in Bouin’s solution and subsequently observed for external and internal malformation or in alcohol for skeleton malformation. The skeletons were stained with alizarin red-S. The examination showed an insignificant (P < 0.05) number of skeleton malformations, external haematomas and any internal malformations.
Get Citation

Keywords

caffeine; congenital-malformation; pregnancy; rat

About this article
Title

The effects of short-time caffeine administration on skeleton development in Wistar rats

Journal

Folia Morphologica

Issue

Vol 59, No 2 (2000)

Pages

91-95

Published online

2000-02-16

Bibliographic record

Folia Morphol 2000;59(2):91-95.

Keywords

caffeine
congenital-malformation
pregnancy
rat

Authors

Franciszek Burdan
Barbara Medej
Zbigniew Wójtowicz
Ryszard Maciejewski

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl