open access

Vol 59, No 4 (2000)
ORIGINAL ARTICLES
Published online: 2000-08-02
Submitted: 2012-02-06
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Immunocytochemical evaluation of reorganisation of keratinocyte cytoskeleton induced by change in Ca 2+ concentration in culture medium

Jolanta Saczko, Elżbieta Gębarowska, Maciej Zabel
Folia Morphol 2000;59(4):247-251.

open access

Vol 59, No 4 (2000)
ORIGINAL ARTICLES
Published online: 2000-08-02
Submitted: 2012-02-06

Abstract

Ca2+ level-induced changes in the arrangement of cytoskeleton of cultured keratinocytes were estimated immunocytochemically, by evaluating expression of specific cytokeratins, desmoplakin and tubulin. Keratinocytes were isolated from fragments of skin of dead human foetuses. Culture of epidermal cells was performed in two phases: phase I yielded cells of high proliferation abilities in serumfree Keratinocyte SFM of low Ca2+ level (0.03 mM); in phase II differentiated cells were obtained in Dulbecco medium of a high Ca2+ concentration (1.2 mM). Immunocytochemical evaluation of phase I and II cells revealed an array of differences which involved mainly expression and distribution of specific cytokeratins, distribution of tubulin, testifying to a different microtubule arrangement and distribution of desmoplakin and indicating a tendency to form desmosomes. The changes were induced by the changes in Ca2+ level in the culture medium.

Abstract

Ca2+ level-induced changes in the arrangement of cytoskeleton of cultured keratinocytes were estimated immunocytochemically, by evaluating expression of specific cytokeratins, desmoplakin and tubulin. Keratinocytes were isolated from fragments of skin of dead human foetuses. Culture of epidermal cells was performed in two phases: phase I yielded cells of high proliferation abilities in serumfree Keratinocyte SFM of low Ca2+ level (0.03 mM); in phase II differentiated cells were obtained in Dulbecco medium of a high Ca2+ concentration (1.2 mM). Immunocytochemical evaluation of phase I and II cells revealed an array of differences which involved mainly expression and distribution of specific cytokeratins, distribution of tubulin, testifying to a different microtubule arrangement and distribution of desmoplakin and indicating a tendency to form desmosomes. The changes were induced by the changes in Ca2+ level in the culture medium.
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Keywords

keratinocytes; culture; cytoskeleton; desmosomes

About this article
Title

Immunocytochemical evaluation of reorganisation of keratinocyte cytoskeleton induced by change in Ca 2+ concentration in culture medium

Journal

Folia Morphologica

Issue

Vol 59, No 4 (2000)

Pages

247-251

Published online

2000-08-02

Bibliographic record

Folia Morphol 2000;59(4):247-251.

Keywords

keratinocytes
culture
cytoskeleton
desmosomes

Authors

Jolanta Saczko
Elżbieta Gębarowska
Maciej Zabel

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