Cholinergic innervation of parvalbumin- and calbindin-containing neurones in the hippocampus during postnatal development of the rat brain

Beata Ludkiewicz; Sławomir Wójcik; Edyta Spodnik; Beata Domaradzka-Pytel; Ilona Klejbor; Janusz Moryś

Vol 61, No 2 (2002)

Original article

Submitted: 2012-02-06

Published online: 2002-02-22

View PDF

Download PDF file

Get Citation

Cholinergic innervation of parvalbumin- and calbindin-containing neurones in the hippocampus during postnatal development of the rat brain

Beata Ludkiewicz, Sławomir Wójcik, Edyta Spodnik, Beata Domaradzka-Pytel, Ilona Klejbor, Janusz Moryś

Folia Morphol 2002;61(2):89-96.

Vol 61, No 2 (2002)

ORIGINAL ARTICLES

Submitted: 2012-02-06

Published online: 2002-02-22

Abstract

Immunohistochemical study of the cholinergic innervation of the parvalbuminand calbindin-containing cells in the hippocampus was conducted on 30 rat brains of various postnatal ages: P0, P4, P7, P14, P21, P30, P60 and P180. Sections with double immunostaining for vesicular acetylcholine transporter (VAChT; the marker of cholinergic cells, fibres and terminals) and parvalbumin (PV) or calbindin (CB) were analysed using confocal laser-scanning microscope. Obtained data demonstrate that the pattern of cholinergic innervation of calbindin- and parvalbumin-immunoreactive hippocampal neurones shows some differences. During development as well as in the adult species cholinergic terminals preferentially innervate CB-containing neurones, while cholinergic terminals on PV-containing cells were observed rarely. Cholinergic endings on the CB-ir neurones are localised both on their somata and dendrites, whereas on PV-ir cells they form synaptic contact predominantly with processes. In spite of the unquestionable cholinergic influence particularly on CB-ir cells, the number of cholinergic endings suggests that this input seems not to be crucial for the activity of the studied cell populations.