open access

Vol 61, No 2 (2002)
ORIGINAL ARTICLES
Published online: 2002-02-22
Submitted: 2012-02-06
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Cholinergic innervation of parvalbumin- and calbindin-containing neurones in the hippocampus during postnatal development of the rat brain

Beata Ludkiewicz, Sławomir Wójcik, Edyta Spodnik, Beata Domaradzka-Pytel, Ilona Klejbor, Janusz Moryś
Folia Morphol 2002;61(2):89-96.

open access

Vol 61, No 2 (2002)
ORIGINAL ARTICLES
Published online: 2002-02-22
Submitted: 2012-02-06

Abstract

Immunohistochemical study of the cholinergic innervation of the parvalbuminand calbindin-containing cells in the hippocampus was conducted on 30 rat brains of various postnatal ages: P0, P4, P7, P14, P21, P30, P60 and P180. Sections with double immunostaining for vesicular acetylcholine transporter (VAChT; the marker of cholinergic cells, fibres and terminals) and parvalbumin (PV) or calbindin (CB) were analysed using confocal laser-scanning microscope. Obtained data demonstrate that the pattern of cholinergic innervation of calbindin- and parvalbumin-immunoreactive hippocampal neurones shows some differences. During development as well as in the adult species cholinergic terminals preferentially innervate CB-containing neurones, while cholinergic terminals on PV-containing cells were observed rarely. Cholinergic endings on the CB-ir neurones are localised both on their somata and dendrites, whereas on PV-ir cells they form synaptic contact predominantly with processes. In spite of the unquestionable cholinergic influence particularly on CB-ir cells, the number of cholinergic endings suggests that this input seems not to be crucial for the activity of the studied cell populations.

Abstract

Immunohistochemical study of the cholinergic innervation of the parvalbuminand calbindin-containing cells in the hippocampus was conducted on 30 rat brains of various postnatal ages: P0, P4, P7, P14, P21, P30, P60 and P180. Sections with double immunostaining for vesicular acetylcholine transporter (VAChT; the marker of cholinergic cells, fibres and terminals) and parvalbumin (PV) or calbindin (CB) were analysed using confocal laser-scanning microscope. Obtained data demonstrate that the pattern of cholinergic innervation of calbindin- and parvalbumin-immunoreactive hippocampal neurones shows some differences. During development as well as in the adult species cholinergic terminals preferentially innervate CB-containing neurones, while cholinergic terminals on PV-containing cells were observed rarely. Cholinergic endings on the CB-ir neurones are localised both on their somata and dendrites, whereas on PV-ir cells they form synaptic contact predominantly with processes. In spite of the unquestionable cholinergic influence particularly on CB-ir cells, the number of cholinergic endings suggests that this input seems not to be crucial for the activity of the studied cell populations.
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Keywords

hippocampus; development; calbindin; parvalbumin; cholinergic system

About this article
Title

Cholinergic innervation of parvalbumin- and calbindin-containing neurones in the hippocampus during postnatal development of the rat brain

Journal

Folia Morphologica

Issue

Vol 61, No 2 (2002)

Pages

89-96

Published online

2002-02-22

Bibliographic record

Folia Morphol 2002;61(2):89-96.

Keywords

hippocampus
development
calbindin
parvalbumin
cholinergic system

Authors

Beata Ludkiewicz
Sławomir Wójcik
Edyta Spodnik
Beata Domaradzka-Pytel
Ilona Klejbor
Janusz Moryś

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