open access

Vol 62, No 1 (2003)
ORIGINAL ARTICLES
Published online: 2002-12-04
Submitted: 2012-02-06
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Histomorphometric study of megakaryocytes in bone marrow in selected myeloproliferative and lymphoproliferative diseases

Dorota Lemancewicz, Janusz Dzięcioł, Janusz Kłoczko, Elżbieta Litwiejko-Pietryńczak, Wanda Bogusłowicz
Folia Morphol 2003;62(1):51-56.

open access

Vol 62, No 1 (2003)
ORIGINAL ARTICLES
Published online: 2002-12-04
Submitted: 2012-02-06

Abstract

The aim of the study was an estimation of the histomorphometry of megakaryocytes (MK) in bone marrow in selected myeloproliferative and lymphoproliferative diseases. Bone marrow specimens were obtained by trephine biopsies from 41 patients with polycythaemia rubra vera (PV), idiopathic myelofibrosis (MF), chronic lymphocytic leukaemia (CLL), hairy cell leukaemia (HCL) and diffuse large B-cell lymphoma (L). Morphometric evaluation was performed using a standard program set MicroImage (OLYMPUS). The greatest number of typical nucleated MK, "naked" nuclei, anucleated cytoplasmic fragments and the largest area were found in PV. The circular deviation factor of MK and their nuclei increased in all cases. The greatest number of clusters was observed in PV and HCL. A significant increase in the number of dysplastic and "naked" nuclei of MK was noted in all selected haematological diseases. The presence of neoplastic cells in bone marrow increased the morphological changes in MK. Quantitative and morphometrical significant differentiation of MK in separate microscopic field in the same slides confirms the necessity of performing trephine biopsies in each patient with haematological disorders.

Abstract

The aim of the study was an estimation of the histomorphometry of megakaryocytes (MK) in bone marrow in selected myeloproliferative and lymphoproliferative diseases. Bone marrow specimens were obtained by trephine biopsies from 41 patients with polycythaemia rubra vera (PV), idiopathic myelofibrosis (MF), chronic lymphocytic leukaemia (CLL), hairy cell leukaemia (HCL) and diffuse large B-cell lymphoma (L). Morphometric evaluation was performed using a standard program set MicroImage (OLYMPUS). The greatest number of typical nucleated MK, "naked" nuclei, anucleated cytoplasmic fragments and the largest area were found in PV. The circular deviation factor of MK and their nuclei increased in all cases. The greatest number of clusters was observed in PV and HCL. A significant increase in the number of dysplastic and "naked" nuclei of MK was noted in all selected haematological diseases. The presence of neoplastic cells in bone marrow increased the morphological changes in MK. Quantitative and morphometrical significant differentiation of MK in separate microscopic field in the same slides confirms the necessity of performing trephine biopsies in each patient with haematological disorders.
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Keywords

trephine biopsy; histomorphometric features; haematological disorders

About this article
Title

Histomorphometric study of megakaryocytes in bone marrow in selected myeloproliferative and lymphoproliferative diseases

Journal

Folia Morphologica

Issue

Vol 62, No 1 (2003)

Pages

51-56

Published online

2002-12-04

Bibliographic record

Folia Morphol 2003;62(1):51-56.

Keywords

trephine biopsy
histomorphometric features
haematological disorders

Authors

Dorota Lemancewicz
Janusz Dzięcioł
Janusz Kłoczko
Elżbieta Litwiejko-Pietryńczak
Wanda Bogusłowicz

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