Vol 62, No 1 (2003)
Original article
Submitted: 2012-02-06
Published online: 2002-12-04
Histomorphometric study of megakaryocytes in bone marrow in selected myeloproliferative and lymphoproliferative diseases
Dorota Lemancewicz, Janusz Dzięcioł, Janusz Kłoczko, Elżbieta Litwiejko-Pietryńczak, Wanda Bogusłowicz
Folia Morphol 2003;62(1):51-56.
Vol 62, No 1 (2003)
ORIGINAL ARTICLES
Submitted: 2012-02-06
Published online: 2002-12-04
Abstract
The aim of the study was an estimation of the histomorphometry of megakaryocytes (MK) in bone marrow in selected myeloproliferative and lymphoproliferative diseases. Bone marrow specimens were obtained by trephine biopsies from 41 patients with polycythaemia rubra vera (PV), idiopathic myelofibrosis (MF),
chronic lymphocytic leukaemia (CLL), hairy cell leukaemia (HCL) and diffuse large B-cell lymphoma (L). Morphometric evaluation was performed using a standard program set MicroImage (OLYMPUS). The greatest number of typical nucleated MK, "naked" nuclei, anucleated cytoplasmic fragments and the largest area were found in PV. The circular deviation factor of MK and their nuclei increased in all cases. The greatest number of clusters was observed in PV and HCL. A significant
increase in the number of dysplastic and "naked" nuclei of MK was noted
in all selected haematological diseases. The presence of neoplastic cells in bone marrow increased the morphological changes in MK. Quantitative and morphometrical
significant differentiation of MK in separate microscopic field in the
same slides confirms the necessity of performing trephine biopsies in each patient with haematological disorders.
Abstract
The aim of the study was an estimation of the histomorphometry of megakaryocytes (MK) in bone marrow in selected myeloproliferative and lymphoproliferative diseases. Bone marrow specimens were obtained by trephine biopsies from 41 patients with polycythaemia rubra vera (PV), idiopathic myelofibrosis (MF),
chronic lymphocytic leukaemia (CLL), hairy cell leukaemia (HCL) and diffuse large B-cell lymphoma (L). Morphometric evaluation was performed using a standard program set MicroImage (OLYMPUS). The greatest number of typical nucleated MK, "naked" nuclei, anucleated cytoplasmic fragments and the largest area were found in PV. The circular deviation factor of MK and their nuclei increased in all cases. The greatest number of clusters was observed in PV and HCL. A significant
increase in the number of dysplastic and "naked" nuclei of MK was noted
in all selected haematological diseases. The presence of neoplastic cells in bone marrow increased the morphological changes in MK. Quantitative and morphometrical
significant differentiation of MK in separate microscopic field in the
same slides confirms the necessity of performing trephine biopsies in each patient with haematological disorders.
Keywords
trephine biopsy; histomorphometric features; haematological disorders
Title
Histomorphometric study of megakaryocytes in bone marrow in selected myeloproliferative and lymphoproliferative diseases
Journal
Folia Morphologica
Issue
Vol 62, No 1 (2003)
Article type
Original article
Pages
51-56
Published online
2002-12-04
Page views
469
Article views/downloads
1240
Bibliographic record
Folia Morphol 2003;62(1):51-56.
Keywords
trephine biopsy
histomorphometric features
haematological disorders
Authors
Dorota Lemancewicz
Janusz Dzięcioł
Janusz Kłoczko
Elżbieta Litwiejko-Pietryńczak
Wanda Bogusłowicz