open access

Vol 62, No 3 (2003)
SHORT COMMUNICATIONS
Published online: 2003-06-09
Submitted: 2012-02-06
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An ultrastructural demonstration of NADPH-diaphorase/nitric oxide synthase activity in the rat striatal astroglia

Jarosław Całka, Gerald Wolf
Folia Morphol 2003;62(3):251-253.

open access

Vol 62, No 3 (2003)
SHORT COMMUNICATIONS
Published online: 2003-06-09
Submitted: 2012-02-06

Abstract

In the present study we have used electron microscopical NADPH-diaphorase (NADPH-d) histochemistry as a visualization procedure for nitric oxide synthase (NOS) to examine patterns of activity in the subcellular distribution of NADPH-d in the rat striatal astroglia. Electron microscopical examination revealed deposition of the NADPH-d reaction product in a nuclear envelope, fragments of endoplasmic reticulum and mitochondria. Predominantly mitochondria of astrocytic "end feet" were labelled. Our ultrastructural observations promote the possibility that astroglial NADPH-d/NOS is involved in adaptation of the local blood flow in the striatal microenvironment.

Abstract

In the present study we have used electron microscopical NADPH-diaphorase (NADPH-d) histochemistry as a visualization procedure for nitric oxide synthase (NOS) to examine patterns of activity in the subcellular distribution of NADPH-d in the rat striatal astroglia. Electron microscopical examination revealed deposition of the NADPH-d reaction product in a nuclear envelope, fragments of endoplasmic reticulum and mitochondria. Predominantly mitochondria of astrocytic "end feet" were labelled. Our ultrastructural observations promote the possibility that astroglial NADPH-d/NOS is involved in adaptation of the local blood flow in the striatal microenvironment.
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Keywords

NADPH-diaphorase; histochemistry; astrocytes; electron microscopy; nitric oxide synthase

About this article
Title

An ultrastructural demonstration of NADPH-diaphorase/nitric oxide synthase activity in the rat striatal astroglia

Journal

Folia Morphologica

Issue

Vol 62, No 3 (2003)

Pages

251-253

Published online

2003-06-09

Bibliographic record

Folia Morphol 2003;62(3):251-253.

Keywords

NADPH-diaphorase
histochemistry
astrocytes
electron microscopy
nitric oxide synthase

Authors

Jarosław Całka
Gerald Wolf

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