open access

Vol 62, No 3 (2003)
SHORT COMMUNICATIONS
Published online: 2003-06-09
Submitted: 2012-02-06
Get Citation

The influence of inflammation on the expression of neuropeptides in the ileum-projecting primary sensory neurones in the pig

Zenon Pidsudko, Krzysztof Wąsowicz, Waldemar Sienkiewicz, Jerzy Kaleczyc, Krzysztof Czaja, Mirosław Łakomy
Folia Morphol 2003;62(3):235-237.

open access

Vol 62, No 3 (2003)
SHORT COMMUNICATIONS
Published online: 2003-06-09
Submitted: 2012-02-06

Abstract

In the present study the ELISA test was used to investigate the influence of chemically-induced ileitis on the dorsal root ganglia (DRG) neurons in the pig. The preliminary retrograde fluorescent tracing study revealed that ileum-projecting sensory neurones (IPN) are located in the thoracic ganglia (Th; Th8-Th13). The ileum wall in experimental (E) pigs was subjected to multiple injection with 4% paraformaldehyde to induce inflammation, while in the control (C) animals the organ was injected with 0.1 M phosphate buffer. Three days later the DRGs (Th8-Th13) collected from all the animals were evaluated for VIP, SP, CGRP, NPY, GAL and SOM content with an ELISA test. It was found that the inflammation increased clearly the tissue level of SP, GAL and SOM.

Abstract

In the present study the ELISA test was used to investigate the influence of chemically-induced ileitis on the dorsal root ganglia (DRG) neurons in the pig. The preliminary retrograde fluorescent tracing study revealed that ileum-projecting sensory neurones (IPN) are located in the thoracic ganglia (Th; Th8-Th13). The ileum wall in experimental (E) pigs was subjected to multiple injection with 4% paraformaldehyde to induce inflammation, while in the control (C) animals the organ was injected with 0.1 M phosphate buffer. Three days later the DRGs (Th8-Th13) collected from all the animals were evaluated for VIP, SP, CGRP, NPY, GAL and SOM content with an ELISA test. It was found that the inflammation increased clearly the tissue level of SP, GAL and SOM.
Get Citation

Keywords

DRG; ileum; neuropeptides; chemical Inflammation; pig

About this article
Title

The influence of inflammation on the expression of neuropeptides in the ileum-projecting primary sensory neurones in the pig

Journal

Folia Morphologica

Issue

Vol 62, No 3 (2003)

Pages

235-237

Published online

2003-06-09

Bibliographic record

Folia Morphol 2003;62(3):235-237.

Keywords

DRG
ileum
neuropeptides
chemical Inflammation
pig

Authors

Zenon Pidsudko
Krzysztof Wąsowicz
Waldemar Sienkiewicz
Jerzy Kaleczyc
Krzysztof Czaja
Mirosław Łakomy

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl