open access

Vol 62, No 3 (2003)
ORIGINAL ARTICLES
Published online: 2003-06-09
Submitted: 2012-02-06
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Developmental changes in nitric oxide synthase protein expression in the rat striatum and cerebral cortex

Cezary Labuda, Barbara Litwinowicz, Przemysław Kowiański, Jan Henryk Spodnik, Anna Łuczyńska, Janusz Moryś
Folia Morphol 2003;62(3):171-174.

open access

Vol 62, No 3 (2003)
ORIGINAL ARTICLES
Published online: 2003-06-09
Submitted: 2012-02-06

Abstract

We examined the expression of brain nitric oxide synthase (bNOS) in two developing rat brain structures, the striatum and the cerebral cortex. For this purpose, we quantified the relative protein concentration level using the Western blotting method and densitometric scanning. 32 Wistar rats, divided according to survival period (P0-P120-postnatal days) were used in this study. Our results demonstrate that bNOS expression rises in these structures during the first week of postnatal life, reaching a maximum in the striatum on the 10th day and in the cerebral cortex on the 7th day of postnatal life. After the period of increase the expression declines and after the 14th day a stabilisation of bone protein concentration is observed, both in the striatum and the cerebral cortex.
These changes in bone protein expression might be related to the important role of nitric oxide in the developing rat brain, especially in synaptogenesis, apoptosis and neurotransmission.

Abstract

We examined the expression of brain nitric oxide synthase (bNOS) in two developing rat brain structures, the striatum and the cerebral cortex. For this purpose, we quantified the relative protein concentration level using the Western blotting method and densitometric scanning. 32 Wistar rats, divided according to survival period (P0-P120-postnatal days) were used in this study. Our results demonstrate that bNOS expression rises in these structures during the first week of postnatal life, reaching a maximum in the striatum on the 10th day and in the cerebral cortex on the 7th day of postnatal life. After the period of increase the expression declines and after the 14th day a stabilisation of bone protein concentration is observed, both in the striatum and the cerebral cortex.
These changes in bone protein expression might be related to the important role of nitric oxide in the developing rat brain, especially in synaptogenesis, apoptosis and neurotransmission.
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Keywords

nitric oxide; protein expression; Western blotting; development; cerebral cortex; striatum; rat

About this article
Title

Developmental changes in nitric oxide synthase protein expression in the rat striatum and cerebral cortex

Journal

Folia Morphologica

Issue

Vol 62, No 3 (2003)

Pages

171-174

Published online

2003-06-09

Bibliographic record

Folia Morphol 2003;62(3):171-174.

Keywords

nitric oxide
protein expression
Western blotting
development
cerebral cortex
striatum
rat

Authors

Cezary Labuda
Barbara Litwinowicz
Przemysław Kowiański
Jan Henryk Spodnik
Anna Łuczyńska
Janusz Moryś

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