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Effect of hCG on the morphology of rat epididymal epithelial cells in vitro
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Abstract
The aim of the study was to assess the morphology of epididymal epithelial cells in vitro after stimulation with hCG. The experiment was performed on cells isolated from sexually mature rats. The epididymal epithelial cells were cultured in a medium with the addition of dihydrotestosterone (DHT). Moreover, the cells were cultured in the medium with DHT and without DHT but enriched with hCG. The epididymal epithelial cells cultured with DHT formed a monolayer and accumulated glycogen, a PAS-positive substance and lipid droplets. The cells cultured without DHT were stellate in shape and low in glycogen and PASpositive substance but they contained lipid droplets. The morphology of epididymal epithelial cells cultured without DHT but after stimulation with hCG was similar to the morphology of the cells cultured with DHT. This was the first sign that the morphology of the cells can be influenced by hCG.
Abstract
The aim of the study was to assess the morphology of epididymal epithelial cells in vitro after stimulation with hCG. The experiment was performed on cells isolated from sexually mature rats. The epididymal epithelial cells were cultured in a medium with the addition of dihydrotestosterone (DHT). Moreover, the cells were cultured in the medium with DHT and without DHT but enriched with hCG. The epididymal epithelial cells cultured with DHT formed a monolayer and accumulated glycogen, a PAS-positive substance and lipid droplets. The cells cultured without DHT were stellate in shape and low in glycogen and PASpositive substance but they contained lipid droplets. The morphology of epididymal epithelial cells cultured without DHT but after stimulation with hCG was similar to the morphology of the cells cultured with DHT. This was the first sign that the morphology of the cells can be influenced by hCG.
Keywords
epididymal epithelial cells; hCG; DHT; morphology; in vitro
Title
Effect of hCG on the morphology of rat epididymal epithelial cells in vitro
Journal
Issue
Article type
Short communication
Pages
489-491
Published online
2003-09-05
Page views
523
Article views/downloads
1201
Bibliographic record
Folia Morphol 2003;62(4):489-491.
Keywords
epididymal epithelial cells
hCG
DHT
morphology
in vitro
Authors
Małgorzata Świder-Al-Amawi
Mariola Marchlewicz
Barbara Wiszniewska
Lidia Wenda-Różewicka