open access

Vol 62, No 4 (2003)
SHORT COMMUNICATIONS
Published online: 2003-09-05
Submitted: 2012-02-06
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Effect of hCG on the morphology of rat epididymal epithelial cells in vitro

Małgorzata Świder-Al-Amawi, Mariola Marchlewicz, Barbara Wiszniewska, Lidia Wenda-Różewicka
Folia Morphol 2003;62(4):489-491.

open access

Vol 62, No 4 (2003)
SHORT COMMUNICATIONS
Published online: 2003-09-05
Submitted: 2012-02-06

Abstract

The epididymis is an androgen-dependent organ. The hormones regulate the morphology and secretory activity of the epididymal epithelial cells. The cells in vitro resume their function as in vivo and also reveal features of steroidogenic cells. It can be expected that, as with Leydig cells, the morphology and function of the cells can be regulated by LH/hCG.
The aim of the study was to assess the morphology of epididymal epithelial cells in vitro after stimulation with hCG. The experiment was performed on cells isolated from sexually mature rats. The epididymal epithelial cells were cultured in a medium with the addition of dihydrotestosterone (DHT). Moreover, the cells were cultured in the medium with DHT and without DHT but enriched with hCG. The epididymal epithelial cells cultured with DHT formed a monolayer and accumulated glycogen, a PAS-positive substance and lipid droplets. The cells cultured without DHT were stellate in shape and low in glycogen and PASpositive substance but they contained lipid droplets. The morphology of epididymal epithelial cells cultured without DHT but after stimulation with hCG was similar to the morphology of the cells cultured with DHT. This was the first sign that the morphology of the cells can be influenced by hCG.

Abstract

The epididymis is an androgen-dependent organ. The hormones regulate the morphology and secretory activity of the epididymal epithelial cells. The cells in vitro resume their function as in vivo and also reveal features of steroidogenic cells. It can be expected that, as with Leydig cells, the morphology and function of the cells can be regulated by LH/hCG.
The aim of the study was to assess the morphology of epididymal epithelial cells in vitro after stimulation with hCG. The experiment was performed on cells isolated from sexually mature rats. The epididymal epithelial cells were cultured in a medium with the addition of dihydrotestosterone (DHT). Moreover, the cells were cultured in the medium with DHT and without DHT but enriched with hCG. The epididymal epithelial cells cultured with DHT formed a monolayer and accumulated glycogen, a PAS-positive substance and lipid droplets. The cells cultured without DHT were stellate in shape and low in glycogen and PASpositive substance but they contained lipid droplets. The morphology of epididymal epithelial cells cultured without DHT but after stimulation with hCG was similar to the morphology of the cells cultured with DHT. This was the first sign that the morphology of the cells can be influenced by hCG.
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Keywords

epididymal epithelial cells; hCG; DHT; morphology; in vitro

About this article
Title

Effect of hCG on the morphology of rat epididymal epithelial cells in vitro

Journal

Folia Morphologica

Issue

Vol 62, No 4 (2003)

Pages

489-491

Published online

2003-09-05

Bibliographic record

Folia Morphol 2003;62(4):489-491.

Keywords

epididymal epithelial cells
hCG
DHT
morphology
in vitro

Authors

Małgorzata Świder-Al-Amawi
Mariola Marchlewicz
Barbara Wiszniewska
Lidia Wenda-Różewicka

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