open access

Vol 62, No 4 (2003)
Short Communications
Published online: 2003-09-05
Submitted: 2012-02-06
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The expression of calcitonin, calcitonin gene-related peptide and somatostatin in the thyroids of rats of different ages

Jolanta Seidel, Maciej Zabel, Aldona Kasprzak, Rafał Spachacz
Folia Morphol 2003;62(4):485-487.

open access

Vol 62, No 4 (2003)
Short Communications
Published online: 2003-09-05
Submitted: 2012-02-06

Abstract

Using immunocytochemistry and in situ hybridisation expression of calcitonin, calcitonin gene-related peptide (CGRP) and somatostatin was examined in rat thyroids. The immunocytochemical reactions demonstrated the presence of the proteins under investigation at all stages of rat life. Calcitonin and CGRP produced the most numerous parafollicular cells, while somatostatin was present in a few cells only. The number of cells producing the above-mentioned hormones was found to increase in rats with the progressing of age. Hybridocytochemical techniques corroborated the results obtained using immunocytochemical techniques. The most numerous cells were found to contain mRNAs for calcitonin and CGRP. In the case of somatostatin, however, multiple parafollicular cells produced mRNA for the hormone but few cells demonstrated the presence of the corresponding protein.

Abstract

Using immunocytochemistry and in situ hybridisation expression of calcitonin, calcitonin gene-related peptide (CGRP) and somatostatin was examined in rat thyroids. The immunocytochemical reactions demonstrated the presence of the proteins under investigation at all stages of rat life. Calcitonin and CGRP produced the most numerous parafollicular cells, while somatostatin was present in a few cells only. The number of cells producing the above-mentioned hormones was found to increase in rats with the progressing of age. Hybridocytochemical techniques corroborated the results obtained using immunocytochemical techniques. The most numerous cells were found to contain mRNAs for calcitonin and CGRP. In the case of somatostatin, however, multiple parafollicular cells produced mRNA for the hormone but few cells demonstrated the presence of the corresponding protein.
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Keywords

calcitonin; CGRP; somatostatin; immunohistochemistry; in situ hybridisation; thyroid; rat

About this article
Title

The expression of calcitonin, calcitonin gene-related peptide and somatostatin in the thyroids of rats of different ages

Journal

Folia Morphologica

Issue

Vol 62, No 4 (2003)

Pages

485-487

Published online

2003-09-05

Bibliographic record

Folia Morphol 2003;62(4):485-487.

Keywords

calcitonin
CGRP
somatostatin
immunohistochemistry
in situ hybridisation
thyroid
rat

Authors

Jolanta Seidel
Maciej Zabel
Aldona Kasprzak
Rafał Spachacz

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