Vol 62, No 4 (2003)
Short communication
Submitted: 2012-02-06
Published online: 2003-09-05
Bone marrow morphology during haematopoietic stem cell mobilisation with cyclophosphamide in mice
Anna Karbicka, Mariola Marchlewicz, Barbara Wiszniewska, Bogusław Machaliński
Folia Morphol 2003;62(4):435-437.
Vol 62, No 4 (2003)
SHORT COMMUNICATIONS
Submitted: 2012-02-06
Published online: 2003-09-05
Abstract
The aim of the study was to examine the morphology of the bone marrow of
mice after stimulation with cyclophosphamide (Cy). The experimental mice were
given a single intraperitoneal injection with 250 mg/kg bw cyclophosphamide.
After 2, 4 and 6 days of experiment the femurs were obtained for morphological
study. On the 2nd day after the mobilisation of the mice with Cy destruction
of the bone marrow was observed with a decrease in the haematopoietic compartment
and an increase in the area occupied by sinusoids filled with erythrocytes.
Erythrocytes were located among the haematopoietic cells, which indicated
that the endothelial barrier had been disrupted. On the 4th day after treating
the mice with Cy, repair processes in the bone marrow were conducted, including
macrophages. The cells filled with haemosiderin migrated from the extravascular
compartment of the bone marrow into the lumen of the sinusoids. There
were proliferating cells among the haematopoietic cells. On the 6th day the
morphology of the bone marrow was similar to the morphology of that in the
control mice. However, more haematopoietic cells were visible compared to the
control bone marrow. The presence of an increased number of leucocytes in the
sinusoid lumen in comparison with the control suggested that at that time the
migration of haematopoietic cells from the bone marrow had been initiated.
Abstract
The aim of the study was to examine the morphology of the bone marrow of
mice after stimulation with cyclophosphamide (Cy). The experimental mice were
given a single intraperitoneal injection with 250 mg/kg bw cyclophosphamide.
After 2, 4 and 6 days of experiment the femurs were obtained for morphological
study. On the 2nd day after the mobilisation of the mice with Cy destruction
of the bone marrow was observed with a decrease in the haematopoietic compartment
and an increase in the area occupied by sinusoids filled with erythrocytes.
Erythrocytes were located among the haematopoietic cells, which indicated
that the endothelial barrier had been disrupted. On the 4th day after treating
the mice with Cy, repair processes in the bone marrow were conducted, including
macrophages. The cells filled with haemosiderin migrated from the extravascular
compartment of the bone marrow into the lumen of the sinusoids. There
were proliferating cells among the haematopoietic cells. On the 6th day the
morphology of the bone marrow was similar to the morphology of that in the
control mice. However, more haematopoietic cells were visible compared to the
control bone marrow. The presence of an increased number of leucocytes in the
sinusoid lumen in comparison with the control suggested that at that time the
migration of haematopoietic cells from the bone marrow had been initiated.
Keywords
bone marrow morphology; cyclophosphamide; haematopoietic cells; sinusoids
Title
Bone marrow morphology during haematopoietic stem cell mobilisation with cyclophosphamide in mice
Journal
Folia Morphologica
Issue
Vol 62, No 4 (2003)
Article type
Short communication
Pages
435-437
Published online
2003-09-05
Page views
534
Article views/downloads
1114
Bibliographic record
Folia Morphol 2003;62(4):435-437.
Keywords
bone marrow morphology
cyclophosphamide
haematopoietic cells
sinusoids
Authors
Anna Karbicka
Mariola Marchlewicz
Barbara Wiszniewska
Bogusław Machaliński