Vol 62, No 4 (2003)
Short communication
Submitted: 2012-02-06
Published online: 2003-09-05
The morphological evaluation of the homograft wall in an electron microscopic study
Artur Pupka, Barbara Dolińska-Krajewska, Piotr Barć, Jan Skóra, Piotr Szyber
Folia Morphol 2003;62(4):423-425.
Vol 62, No 4 (2003)
SHORT COMMUNICATIONS
Submitted: 2012-02-06
Published online: 2003-09-05
Abstract
Cases of massive purulent infection of vascular prosthesis are demonstrated in
this study. Infected prosthesis was substituted by arterial homograft, harvested
during multi-organ procurement, and stored by the cold ischaemia method. In
the follow-up period, the patients were divided into two groups, those treated
with immunosuppression (n = 16) and those treated without immunosuppressive
drugs (n = 13). The patients underwent resurgery, during which a fragment
of arterial wall was taken for electron microscopic examination.
In the group with immunosuppression, the presence of the following structures
was observed: endothelial cells, the intima, with a great number of elastic and
collagen fibrils with fibrinogen inclusions, and active phagocyting myoblasts and
myofibroblasts. In the group without immunosuppression electron microscopic
examination showed the total destruction of the wall of the ruptured arterial
homograft - absence of endothelium and sparse, damaged fibroblasts of the
media or their degraded fragments, making a picture of cellular death.
Morphological analysis of the arterial wall and the clinical state of the patient
suggest the necessity of immunosuppressive treatment after fresh arterial homograft
transplantation.
Abstract
Cases of massive purulent infection of vascular prosthesis are demonstrated in
this study. Infected prosthesis was substituted by arterial homograft, harvested
during multi-organ procurement, and stored by the cold ischaemia method. In
the follow-up period, the patients were divided into two groups, those treated
with immunosuppression (n = 16) and those treated without immunosuppressive
drugs (n = 13). The patients underwent resurgery, during which a fragment
of arterial wall was taken for electron microscopic examination.
In the group with immunosuppression, the presence of the following structures
was observed: endothelial cells, the intima, with a great number of elastic and
collagen fibrils with fibrinogen inclusions, and active phagocyting myoblasts and
myofibroblasts. In the group without immunosuppression electron microscopic
examination showed the total destruction of the wall of the ruptured arterial
homograft - absence of endothelium and sparse, damaged fibroblasts of the
media or their degraded fragments, making a picture of cellular death.
Morphological analysis of the arterial wall and the clinical state of the patient
suggest the necessity of immunosuppressive treatment after fresh arterial homograft
transplantation.
Keywords
homograft; infection of vascular prosthesis; immunosuppression
Title
The morphological evaluation of the homograft wall in an electron microscopic study
Journal
Folia Morphologica
Issue
Vol 62, No 4 (2003)
Article type
Short communication
Pages
423-425
Published online
2003-09-05
Page views
537
Article views/downloads
1046
Bibliographic record
Folia Morphol 2003;62(4):423-425.
Keywords
homograft
infection of vascular prosthesis
immunosuppression
Authors
Artur Pupka
Barbara Dolińska-Krajewska
Piotr Barć
Jan Skóra
Piotr Szyber