open access

Vol 62, No 4 (2003)
SHORT COMMUNICATIONS
Published online: 2003-09-05
Submitted: 2012-02-06
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An immunohistochemical study of the thyroid parafollicular (C) cells in rats treated with cannabinoids - preliminary investigations

Maria M. Winnicka, Robert Ł. Zbucki, Jacek Dadan, Bogusław Sawicki, Anna Hryniewicz, Piotr Kosiorek, Izabela Bialuk, Zbigniew Puchalski
Folia Morphol 2003;62(4):419-421.

open access

Vol 62, No 4 (2003)
SHORT COMMUNICATIONS
Published online: 2003-09-05
Submitted: 2012-02-06

Abstract

The purpose of the present study was to evaluate the effect of a single intraperitoneal injection of a stable analogue of endogenous cannabinoid anandamide - R-(+)-methanandamide (2.5 mg/kg) and CP 55,940 (0.25 mg/kg), an egzogenous CB1 receptor-agonist, on the calcitonin (CT) immunoreactivity of the thyroid parafollicular (C) cells. Four hours after injection with both cannabinoids CT immunoreactivity, evaluated with an avidin-biotin peroxidase complex method by means of rabbit antibodies against CT, was seen to be enhanced in the parafollicular cells in comparison to those of the control group. In thyroids taken from cannabinoid-treated rats the majority of follicles, particularly those located peripherally were large in size, and had low epithelium. Moreover, dilatation of the blood vessels was observed. These changes were accompanied by a significant decrease in CT plasma level, without changes in calcium concentrations. This is the first evidence that a single injection of the cannabinoids R-(+)-methanandamide and CP 55,940 significantly decreases the activity of thyroid C cells.

Abstract

The purpose of the present study was to evaluate the effect of a single intraperitoneal injection of a stable analogue of endogenous cannabinoid anandamide - R-(+)-methanandamide (2.5 mg/kg) and CP 55,940 (0.25 mg/kg), an egzogenous CB1 receptor-agonist, on the calcitonin (CT) immunoreactivity of the thyroid parafollicular (C) cells. Four hours after injection with both cannabinoids CT immunoreactivity, evaluated with an avidin-biotin peroxidase complex method by means of rabbit antibodies against CT, was seen to be enhanced in the parafollicular cells in comparison to those of the control group. In thyroids taken from cannabinoid-treated rats the majority of follicles, particularly those located peripherally were large in size, and had low epithelium. Moreover, dilatation of the blood vessels was observed. These changes were accompanied by a significant decrease in CT plasma level, without changes in calcium concentrations. This is the first evidence that a single injection of the cannabinoids R-(+)-methanandamide and CP 55,940 significantly decreases the activity of thyroid C cells.
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Keywords

thyroid C cells; cannabinoids; immunohistochemistry; rats

About this article
Title

An immunohistochemical study of the thyroid parafollicular (C) cells in rats treated with cannabinoids - preliminary investigations

Journal

Folia Morphologica

Issue

Vol 62, No 4 (2003)

Pages

419-421

Published online

2003-09-05

Bibliographic record

Folia Morphol 2003;62(4):419-421.

Keywords

thyroid C cells
cannabinoids
immunohistochemistry
rats

Authors

Maria M. Winnicka
Robert Ł. Zbucki
Jacek Dadan
Bogusław Sawicki
Anna Hryniewicz
Piotr Kosiorek
Izabela Bialuk
Zbigniew Puchalski

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