open access

Vol 62, No 4 (2003)
Short Communications
Published online: 2003-09-05
Submitted: 2012-02-06
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Localisation of exogenous surfactants in cell membranes in the air-blood barrier: rat model

Andrzej Marszałek, Wiesława Biczysko, Marcin Wąsowicz, Paweł Surowiak, Maciej Zabel, Ewa Florek
Folia Morphol 2003;62(4):377-379.

open access

Vol 62, No 4 (2003)
Short Communications
Published online: 2003-09-05
Submitted: 2012-02-06

Abstract

The use of exogenous surfactants has been introduced into the therapy of patients of different ages. Much better results have been obtained in the treatment of respiratory distress syndrome with surfactants enriched with surfactant proteins. In the following study we used protein-containing surfactants (survanta and curosurf). The aim of the following study was to determine the localisation of artificial surfactants in the lung tissue. Using the Immunogold Technique, biotinylated surfactant proteins were traced in the air-blood barriers. In all lungs the exogenous surfactant was present only in some alveoli. In these parts small areas of atelectasis as well as oedema and transudate accumulation were seen. These changes were less severe after biotinylated curosurf treatment. In electron microscope studies we found surfactant elements in the air-blood barrier and other structures of the alveolar septa. Immunogold studies confirm the presence of biotynylated surfactant in the elements of the air-blood barrier.

Abstract

The use of exogenous surfactants has been introduced into the therapy of patients of different ages. Much better results have been obtained in the treatment of respiratory distress syndrome with surfactants enriched with surfactant proteins. In the following study we used protein-containing surfactants (survanta and curosurf). The aim of the following study was to determine the localisation of artificial surfactants in the lung tissue. Using the Immunogold Technique, biotinylated surfactant proteins were traced in the air-blood barriers. In all lungs the exogenous surfactant was present only in some alveoli. In these parts small areas of atelectasis as well as oedema and transudate accumulation were seen. These changes were less severe after biotinylated curosurf treatment. In electron microscope studies we found surfactant elements in the air-blood barrier and other structures of the alveolar septa. Immunogold studies confirm the presence of biotynylated surfactant in the elements of the air-blood barrier.
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Keywords

exogenous surfactant; lung; electron microscopy; immunogold

About this article
Title

Localisation of exogenous surfactants in cell membranes in the air-blood barrier: rat model

Journal

Folia Morphologica

Issue

Vol 62, No 4 (2003)

Pages

377-379

Published online

2003-09-05

Bibliographic record

Folia Morphol 2003;62(4):377-379.

Keywords

exogenous surfactant
lung
electron microscopy
immunogold

Authors

Andrzej Marszałek
Wiesława Biczysko
Marcin Wąsowicz
Paweł Surowiak
Maciej Zabel
Ewa Florek

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