Vol 62, No 4 (2003)
Short communication
Published online: 2003-09-05
Localisation of exogenous surfactants in cell membranes in the air-blood barrier: rat model
Folia Morphol 2003;62(4):377-379.
Abstract
The use of exogenous surfactants has been introduced into the therapy of patients
of different ages. Much better results have been obtained in the treatment
of respiratory distress syndrome with surfactants enriched with surfactant
proteins. In the following study we used protein-containing surfactants (survanta
and curosurf). The aim of the following study was to determine the localisation
of artificial surfactants in the lung tissue. Using the Immunogold Technique,
biotinylated surfactant proteins were traced in the air-blood barriers. In all lungs
the exogenous surfactant was present only in some alveoli. In these parts small
areas of atelectasis as well as oedema and transudate accumulation were seen.
These changes were less severe after biotinylated curosurf treatment. In electron
microscope studies we found surfactant elements in the air-blood barrier
and other structures of the alveolar septa. Immunogold studies confirm the presence
of biotynylated surfactant in the elements of the air-blood barrier.
Keywords: exogenous surfactantlungelectron microscopyimmunogold
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