open access

Vol 62, No 4 (2003)
ORIGINAL ARTICLES
Published online: 2003-09-05
Submitted: 2012-02-06
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Developmental pattern of calbindin D28k protein expression in the rat striatum and cerebral cortex

Barbara Litwinowicz, Cezary Labuda, Przemysław Kowiański, Jan H. Spodnik, Beata Ludkiewicz, Sławomir Wójcik, Janusz Moryś
Folia Morphol 2003;62(4):327-329.

open access

Vol 62, No 4 (2003)
ORIGINAL ARTICLES
Published online: 2003-09-05
Submitted: 2012-02-06

Abstract

We examined the protein expression of the calcium-binding protein calbindin D28k in two developing rat brain structures, the striatum and the cerebral cortex. The relative protein concentration level was quantified by means of the Western blotting method and densitometric scanning. 32 Wistar rats were used, divided according to survival period (P0-P120-postnatal days). Observations of the calbindin D28k protein expression in the rat striatum and the cerebral cortex revealed an increase in band color intensity between P0 and P10. The intensity of protein staining in older groups of animals stabilised at a similar level and in the P28 and P120 groups we observed a decrement of calbindin expression in the striatum.
Calbindin D28k stabilises the intracellular calcium level, preventing calcium-induced apoptotic cell death in neurons. Thus, changes in calbindin D28k expression might be related to its neuroprotective role in differentiation and synaptogenesis during the postnatal development of the brain.

Abstract

We examined the protein expression of the calcium-binding protein calbindin D28k in two developing rat brain structures, the striatum and the cerebral cortex. The relative protein concentration level was quantified by means of the Western blotting method and densitometric scanning. 32 Wistar rats were used, divided according to survival period (P0-P120-postnatal days). Observations of the calbindin D28k protein expression in the rat striatum and the cerebral cortex revealed an increase in band color intensity between P0 and P10. The intensity of protein staining in older groups of animals stabilised at a similar level and in the P28 and P120 groups we observed a decrement of calbindin expression in the striatum.
Calbindin D28k stabilises the intracellular calcium level, preventing calcium-induced apoptotic cell death in neurons. Thus, changes in calbindin D28k expression might be related to its neuroprotective role in differentiation and synaptogenesis during the postnatal development of the brain.
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Keywords

calbindin D28k; protein expression; Western blotting; development; cerebral cortex; striatum; rat

About this article
Title

Developmental pattern of calbindin D28k protein expression in the rat striatum and cerebral cortex

Journal

Folia Morphologica

Issue

Vol 62, No 4 (2003)

Pages

327-329

Published online

2003-09-05

Bibliographic record

Folia Morphol 2003;62(4):327-329.

Keywords

calbindin D28k
protein expression
Western blotting
development
cerebral cortex
striatum
rat

Authors

Barbara Litwinowicz
Cezary Labuda
Przemysław Kowiański
Jan H. Spodnik
Beata Ludkiewicz
Sławomir Wójcik
Janusz Moryś

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