Vol 63, No 2 (2004)
Short communication
Published online: 2004-03-12
Immunohistochemical and electron-microscopical identification of neuroendocrine cells in the respiratory tract of rats with experimental uraemia
Folia Morphol 2004;63(2):233-235.
Abstract
The aim of the present study was to characterise immunohistochemically and
ultrastructurally the neuroendocrine cells in the pulmonary systems of uraemic
rats. Pieces of lung and trachea were collected 1, 2 and 4 weeks after nephrectomy.
Paraffin-embedded sections were stained with H+E and by silver impregnation.
For the identification of neuroendocrine cells immunohistochemical reactions
were performed with the use of specific antibodies against calcitonin
(CT), somatostatin (SOM), synaptophysin (SYN), neuron-specific enolase (NSE)
and calcitonin gene-related peptide (CGRP). For electron microscopy, ultrathin
sections were used. The analysis showed an increased number of both the solitary
neuroendocrine cells and of neuroepithelial bodies in uraemic rats when
compared to control animals.
Keywords: APUD cellslungtrachearatsuraemia