open access

Vol 63, No 2 (2004)
Short Communications
Published online: 2004-03-12
Submitted: 2012-02-06
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Immunohistochemical and electron-microscopical identification of neuroendocrine cells in the respiratory tract of rats with experimental uraemia

Kasacka I, Sawicki B
Folia Morphol 2004;63(2):233-235.

open access

Vol 63, No 2 (2004)
Short Communications
Published online: 2004-03-12
Submitted: 2012-02-06

Abstract

The aim of the present study was to characterise immunohistochemically and ultrastructurally the neuroendocrine cells in the pulmonary systems of uraemic rats. Pieces of lung and trachea were collected 1, 2 and 4 weeks after nephrectomy. Paraffin-embedded sections were stained with H+E and by silver impregnation. For the identification of neuroendocrine cells immunohistochemical reactions were performed with the use of specific antibodies against calcitonin (CT), somatostatin (SOM), synaptophysin (SYN), neuron-specific enolase (NSE) and calcitonin gene-related peptide (CGRP). For electron microscopy, ultrathin sections were used. The analysis showed an increased number of both the solitary neuroendocrine cells and of neuroepithelial bodies in uraemic rats when compared to control animals.

Abstract

The aim of the present study was to characterise immunohistochemically and ultrastructurally the neuroendocrine cells in the pulmonary systems of uraemic rats. Pieces of lung and trachea were collected 1, 2 and 4 weeks after nephrectomy. Paraffin-embedded sections were stained with H+E and by silver impregnation. For the identification of neuroendocrine cells immunohistochemical reactions were performed with the use of specific antibodies against calcitonin (CT), somatostatin (SOM), synaptophysin (SYN), neuron-specific enolase (NSE) and calcitonin gene-related peptide (CGRP). For electron microscopy, ultrathin sections were used. The analysis showed an increased number of both the solitary neuroendocrine cells and of neuroepithelial bodies in uraemic rats when compared to control animals.
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Keywords

APUD cells; lung; trachea; rats; uraemia

About this article
Title

Immunohistochemical and electron-microscopical identification of neuroendocrine cells in the respiratory tract of rats with experimental uraemia

Journal

Folia Morphologica

Issue

Vol 63, No 2 (2004)

Pages

233-235

Published online

2004-03-12

Bibliographic record

Folia Morphol 2004;63(2):233-235.

Keywords

APUD cells
lung
trachea
rats
uraemia

Authors

Kasacka I
Sawicki B

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