Vol 63, No 2 (2004)
Short communication
Submitted: 2012-02-06
Published online: 2004-03-12
The detection of Helicobacter pylori in paraffin sections using the PCR technique and various primers as compared to histological techniques
Ciesielska U, Dzięgiel P, Jagoda E, Podhorska-Okołów M, Zabel M
Folia Morphol 2004;63(2):229-231.
Vol 63, No 2 (2004)
SHORT COMMUNICATIONS
Submitted: 2012-02-06
Published online: 2004-03-12
Abstract
Helicobacter pylori is thought to represent a significant etiopathogenic factor in
diseases of the upper gastrointestinal tract. It seems, therefore, important to elaborate
effective techniques for its detection. The aim of the present study was to
evaluate the effectiveness of Helicobacter pylori detection using the PCR technique
on paraffin sections with various pairs of primers and to compare the results
with those of a histological appraisal. Material for the studies involved 50 paraffin
blocks with gastric mucosa biopsies fixed in 4% buffered formalin. In this material
4 tests were performed with the aim of diagnosing Helicobacter pylori infection:
1) H+E staining, 2) staining by the Giemsa technique, 3) an immunocytochemical
technique with antibodies against H. pylori and 4) the PCR technique with various
primers. In the present study the most reliable results for H. pylori detection as
well as the most pronounced correlation were obtained by using the PCR technique
with primers for the ureC gene, immunohistochemistry and staining according
to Giemsa. Less compatible results were obtained employing the two PCR
techniques which utilise various primers. The experiments confirmed the usefulness
of the PCR technique in the detection of Helicobacter pylori in paraffin sections
by using a suitable pair of primers, and also indicated that Giemsa staining
and immunohistochemistry should be taken into account.
Abstract
Helicobacter pylori is thought to represent a significant etiopathogenic factor in
diseases of the upper gastrointestinal tract. It seems, therefore, important to elaborate
effective techniques for its detection. The aim of the present study was to
evaluate the effectiveness of Helicobacter pylori detection using the PCR technique
on paraffin sections with various pairs of primers and to compare the results
with those of a histological appraisal. Material for the studies involved 50 paraffin
blocks with gastric mucosa biopsies fixed in 4% buffered formalin. In this material
4 tests were performed with the aim of diagnosing Helicobacter pylori infection:
1) H+E staining, 2) staining by the Giemsa technique, 3) an immunocytochemical
technique with antibodies against H. pylori and 4) the PCR technique with various
primers. In the present study the most reliable results for H. pylori detection as
well as the most pronounced correlation were obtained by using the PCR technique
with primers for the ureC gene, immunohistochemistry and staining according
to Giemsa. Less compatible results were obtained employing the two PCR
techniques which utilise various primers. The experiments confirmed the usefulness
of the PCR technique in the detection of Helicobacter pylori in paraffin sections
by using a suitable pair of primers, and also indicated that Giemsa staining
and immunohistochemistry should be taken into account.
Keywords
PCR technique; Helicobacter pylori infection; detection
Title
The detection of Helicobacter pylori in paraffin sections using the PCR technique and various primers as compared to histological techniques
Journal
Folia Morphologica
Issue
Vol 63, No 2 (2004)
Article type
Short communication
Pages
229-231
Published online
2004-03-12
Page views
458
Article views/downloads
1463
Bibliographic record
Folia Morphol 2004;63(2):229-231.
Keywords
PCR technique
Helicobacter pylori infection
detection
Authors
Ciesielska U
Dzięgiel P
Jagoda E
Podhorska-Okołów M
Zabel M