Vol 63, No 2 (2004)
Short communication
Published online: 2004-03-12
The detection of Helicobacter pylori in paraffin sections using the PCR technique and various primers as compared to histological techniques
Folia Morphol 2004;63(2):229-231.
Abstract
Helicobacter pylori is thought to represent a significant etiopathogenic factor in
diseases of the upper gastrointestinal tract. It seems, therefore, important to elaborate
effective techniques for its detection. The aim of the present study was to
evaluate the effectiveness of Helicobacter pylori detection using the PCR technique
on paraffin sections with various pairs of primers and to compare the results
with those of a histological appraisal. Material for the studies involved 50 paraffin
blocks with gastric mucosa biopsies fixed in 4% buffered formalin. In this material
4 tests were performed with the aim of diagnosing Helicobacter pylori infection:
1) H+E staining, 2) staining by the Giemsa technique, 3) an immunocytochemical
technique with antibodies against H. pylori and 4) the PCR technique with various
primers. In the present study the most reliable results for H. pylori detection as
well as the most pronounced correlation were obtained by using the PCR technique
with primers for the ureC gene, immunohistochemistry and staining according
to Giemsa. Less compatible results were obtained employing the two PCR
techniques which utilise various primers. The experiments confirmed the usefulness
of the PCR technique in the detection of Helicobacter pylori in paraffin sections
by using a suitable pair of primers, and also indicated that Giemsa staining
and immunohistochemistry should be taken into account.
Keywords: PCR techniqueHelicobacter pylori infectiondetection
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