open access

Vol 63, No 3 (2004)
ORIGINAL ARTICLES
Published online: 2004-06-04
Submitted: 2012-02-06
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Developmental expression of SNAP-25 protein in the rat striatum and cerebral cortex

J Sidor-Kaczmarek, C Labuda, B Litwinowicz, JH Spodnik, P Kowiański, J Dziewiątkowski, J Moryś
Folia Morphol 2004;63(3):285-288.

open access

Vol 63, No 3 (2004)
ORIGINAL ARTICLES
Published online: 2004-06-04
Submitted: 2012-02-06

Abstract

The developmental changes of 25-kDa synaptosomal-associated protein (SNAP-25) expression in the rat striatum and cerebral cortex were examined using Western- blotting and densitometric scanning of immunoblots. Analysis of the striatum extracts from postnatal day 0 (P0) to postnatal day 120 (P120) demonstrated that SNAP-25 is poorly expressed until P14. From this point the expression level gradually increases to reach a maximum on P60 and then decreases. The pattern of SNAP-25 expression in the rat cerebral cortex is different. Two peaks are observed, the first on P10 and the second on P60, after which the expression level decreases. These results appear to confirm the role of SNAP-25 protein in axon outgrowth and synaptogenesis in the nervous system.

Abstract

The developmental changes of 25-kDa synaptosomal-associated protein (SNAP-25) expression in the rat striatum and cerebral cortex were examined using Western- blotting and densitometric scanning of immunoblots. Analysis of the striatum extracts from postnatal day 0 (P0) to postnatal day 120 (P120) demonstrated that SNAP-25 is poorly expressed until P14. From this point the expression level gradually increases to reach a maximum on P60 and then decreases. The pattern of SNAP-25 expression in the rat cerebral cortex is different. Two peaks are observed, the first on P10 and the second on P60, after which the expression level decreases. These results appear to confirm the role of SNAP-25 protein in axon outgrowth and synaptogenesis in the nervous system.
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Keywords

SNAP-25; protein expression; Western blotting; development; cerebral cortex; striatum; rat

About this article
Title

Developmental expression of SNAP-25 protein in the rat striatum and cerebral cortex

Journal

Folia Morphologica

Issue

Vol 63, No 3 (2004)

Pages

285-288

Published online

2004-06-04

Bibliographic record

Folia Morphol 2004;63(3):285-288.

Keywords

SNAP-25
protein expression
Western blotting
development
cerebral cortex
striatum
rat

Authors

J Sidor-Kaczmarek
C Labuda
B Litwinowicz
JH Spodnik
P Kowiański
J Dziewiątkowski
J Moryś

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