open access

Vol 63, No 3 (2004)
Original article
Submitted: 2012-02-06
Published online: 2004-06-04
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4-OH-TEMPO prevents the morphological alteration of rat thymocytes primed to apoptosis by oxidative stress inducer ButOOH

E Kossowka, A Zauszkiewicz, J Kubasik-Juraniec, C Tukaj, JH Spodnik, A Hallman, J Klimek, E Syta, A Figarski, T Wakabayashi, M Woźniak
Folia Morphol 2004;63(3):261-265.

open access

Vol 63, No 3 (2004)
ORIGINAL ARTICLES
Submitted: 2012-02-06
Published online: 2004-06-04

Abstract

Thymocytes exposed to the pro-oxidant tert–butyl-hydroperoxide (ButOOH) display a number of dramatic changes in morphology similar to those observed in the case of dexamethasone-treated cells. Both reagents induce nuclear chromatin peripheral aggregation below the nuclear membrane. Some nuclei themselves break up producing two or more fragments. ButOOH-treated cells are morphologically characterised by cell shrinkage, extensive surface blebbing and, finally, fragmentation into membrane–bound apoptotic bodies composed of cytoplasm and tightly packed with or without nuclear fragments. An increased level of lipid hydroxyperoxides was detected after exposure of thymocytes to ButOOH. Both oxidative stress markers and morphological damage to cells were prevented by the antioxidant 4-OH-TEMPO.

Abstract

Thymocytes exposed to the pro-oxidant tert–butyl-hydroperoxide (ButOOH) display a number of dramatic changes in morphology similar to those observed in the case of dexamethasone-treated cells. Both reagents induce nuclear chromatin peripheral aggregation below the nuclear membrane. Some nuclei themselves break up producing two or more fragments. ButOOH-treated cells are morphologically characterised by cell shrinkage, extensive surface blebbing and, finally, fragmentation into membrane–bound apoptotic bodies composed of cytoplasm and tightly packed with or without nuclear fragments. An increased level of lipid hydroxyperoxides was detected after exposure of thymocytes to ButOOH. Both oxidative stress markers and morphological damage to cells were prevented by the antioxidant 4-OH-TEMPO.
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Keywords

4-OH-TEMPO; apoptosis; thymocytes; oxidative stress

About this article
Title

4-OH-TEMPO prevents the morphological alteration of rat thymocytes primed to apoptosis by oxidative stress inducer ButOOH

Journal

Folia Morphologica

Issue

Vol 63, No 3 (2004)

Article type

Original article

Pages

261-265

Published online

2004-06-04

Page views

548

Article views/downloads

1065

Bibliographic record

Folia Morphol 2004;63(3):261-265.

Keywords

4-OH-TEMPO
apoptosis
thymocytes
oxidative stress

Authors

E Kossowka
A Zauszkiewicz
J Kubasik-Juraniec
C Tukaj
JH Spodnik
A Hallman
J Klimek
E Syta
A Figarski
T Wakabayashi
M Woźniak

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