open access

Vol 63, No 3 (2004)
ORIGINAL ARTICLES
Published online: 2004-06-04
Submitted: 2012-02-06
Get Citation

4-OH-TEMPO prevents the morphological alteration of rat thymocytes primed to apoptosis by oxidative stress inducer ButOOH

E Kossowka, A Zauszkiewicz, J Kubasik-Juraniec, C Tukaj, JH Spodnik, A Hallman, J Klimek, E Syta, A Figarski, T Wakabayashi, M Woźniak
Folia Morphol 2004;63(3):261-265.

open access

Vol 63, No 3 (2004)
ORIGINAL ARTICLES
Published online: 2004-06-04
Submitted: 2012-02-06

Abstract

Thymocytes exposed to the pro-oxidant tert–butyl-hydroperoxide (ButOOH) display a number of dramatic changes in morphology similar to those observed in the case of dexamethasone-treated cells. Both reagents induce nuclear chromatin peripheral aggregation below the nuclear membrane. Some nuclei themselves break up producing two or more fragments. ButOOH-treated cells are morphologically characterised by cell shrinkage, extensive surface blebbing and, finally, fragmentation into membrane–bound apoptotic bodies composed of cytoplasm and tightly packed with or without nuclear fragments. An increased level of lipid hydroxyperoxides was detected after exposure of thymocytes to ButOOH. Both oxidative stress markers and morphological damage to cells were prevented by the antioxidant 4-OH-TEMPO.

Abstract

Thymocytes exposed to the pro-oxidant tert–butyl-hydroperoxide (ButOOH) display a number of dramatic changes in morphology similar to those observed in the case of dexamethasone-treated cells. Both reagents induce nuclear chromatin peripheral aggregation below the nuclear membrane. Some nuclei themselves break up producing two or more fragments. ButOOH-treated cells are morphologically characterised by cell shrinkage, extensive surface blebbing and, finally, fragmentation into membrane–bound apoptotic bodies composed of cytoplasm and tightly packed with or without nuclear fragments. An increased level of lipid hydroxyperoxides was detected after exposure of thymocytes to ButOOH. Both oxidative stress markers and morphological damage to cells were prevented by the antioxidant 4-OH-TEMPO.
Get Citation

Keywords

4-OH-TEMPO; apoptosis; thymocytes; oxidative stress

About this article
Title

4-OH-TEMPO prevents the morphological alteration of rat thymocytes primed to apoptosis by oxidative stress inducer ButOOH

Journal

Folia Morphologica

Issue

Vol 63, No 3 (2004)

Pages

261-265

Published online

2004-06-04

Bibliographic record

Folia Morphol 2004;63(3):261-265.

Keywords

4-OH-TEMPO
apoptosis
thymocytes
oxidative stress

Authors

E Kossowka
A Zauszkiewicz
J Kubasik-Juraniec
C Tukaj
JH Spodnik
A Hallman
J Klimek
E Syta
A Figarski
T Wakabayashi
M Woźniak

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl