Vol 63, No 4 (2004)
Original article
Submitted: 2012-02-06
Published online: 2004-09-16
The influence of chemotherapy on bone marrow in patients with chronic lymphocytic leukaemia
Dorota Lemancewicz, Janusz Dzięcioł, Janusz Kłoczko, Jarosław Piszcz
Folia Morphol 2004;63(4):485-489.
Vol 63, No 4 (2004)
ORIGINAL ARTICLES
Submitted: 2012-02-06
Published online: 2004-09-16
Abstract
In chronic lymphocytic leukaemia (CLL) bone marrow trephine biopsy (BMT) is not
required for diagnosis but can have a significant prognostic value and can be used
for the detection of the minimal residual disease (MRD) and for assessment of the
effectiveness of the treatment applied. The aim of the study was to evaluate the
morphological changes in bone marrow after treatment with purine nucleoside
analogues cladribine and fludarabine. Bone marrow trephine biopsy was taken
routinely from 15 patients with CLL. Bone marrow trephine biopsy was performed
on every patient before as well as after chemotherapy. The number of cell elements
of the marrow (the degree of atrophy), the patterns of bone marrow infiltration,
the presence of reticulin and collagen fibres and the disturbances in bone
marrow stroma were assessed. The infiltration of bone marrow by neoplastic cells
was observed in all the patients before administration of chemotherapy. The infiltration
was followed by an increase in the number of reticulin fibres. After the
treatment a regression of the reticulin fibres was observed with the lessening of
the infiltration. After the treatment the levels of marrow infiltrate were decreased.
Increased hypoplasia of the bone marrow was observed after the chemotherapy.
Abstract
In chronic lymphocytic leukaemia (CLL) bone marrow trephine biopsy (BMT) is not
required for diagnosis but can have a significant prognostic value and can be used
for the detection of the minimal residual disease (MRD) and for assessment of the
effectiveness of the treatment applied. The aim of the study was to evaluate the
morphological changes in bone marrow after treatment with purine nucleoside
analogues cladribine and fludarabine. Bone marrow trephine biopsy was taken
routinely from 15 patients with CLL. Bone marrow trephine biopsy was performed
on every patient before as well as after chemotherapy. The number of cell elements
of the marrow (the degree of atrophy), the patterns of bone marrow infiltration,
the presence of reticulin and collagen fibres and the disturbances in bone
marrow stroma were assessed. The infiltration of bone marrow by neoplastic cells
was observed in all the patients before administration of chemotherapy. The infiltration
was followed by an increase in the number of reticulin fibres. After the
treatment a regression of the reticulin fibres was observed with the lessening of
the infiltration. After the treatment the levels of marrow infiltrate were decreased.
Increased hypoplasia of the bone marrow was observed after the chemotherapy.
Keywords
bone marrow trephine biopsy; lymphoid infiltration; reticulin fibrosis
Title
The influence of chemotherapy on bone marrow in patients with chronic lymphocytic leukaemia
Journal
Folia Morphologica
Issue
Vol 63, No 4 (2004)
Article type
Original article
Pages
485-489
Published online
2004-09-16
Page views
914
Article views/downloads
1558
Bibliographic record
Folia Morphol 2004;63(4):485-489.
Keywords
bone marrow trephine biopsy
lymphoid infiltration
reticulin fibrosis
Authors
Dorota Lemancewicz
Janusz Dzięcioł
Janusz Kłoczko
Jarosław Piszcz