open access

Vol 63, No 4 (2004)
ORIGINAL ARTICLES
Published online: 2004-09-16
Submitted: 2012-02-06
Get Citation

Fast perinuclear clustering of mitochondria in oxidatively stressed human choriocarcinoma cells

Anna Hallmann, Ryszard Milczarek, Marcin Lipiński, Ewa Kossowska, Jan Henryk Spodnik, Michał Woźniak, Takashi Wakabayashi, Jerzy Klimek
Folia Morphol 2004;63(4):407-412.

open access

Vol 63, No 4 (2004)
ORIGINAL ARTICLES
Published online: 2004-09-16
Submitted: 2012-02-06

Abstract

Mitochondrial dysfunction plays a crucial role in cell types that exhibit necrosislike death after activation of their death program. Tumour necrosis factor (TNF) induces abnormal, perinuclear clustering of mitochondria from an evenly spread distribution throughout the cytoplasm. The mitochondria withdraw from the cell periphery and aggregate in a unipolar perinuclear cluster. TNF-induced mitochondrial clustering is caused by impaired kinesin-mediated transportation of mitochondria. In this report, we describe a novel activity of menadione (MEN), namely the induction of an altered spatial distribution of mitochondria in the choriocarcinoma JAR cells. Strikingly, 2 hours of cell exposition to menadione did not disrupt the integrity of the plasma membrane, while the intracellular ATP level significantly decreased. Control (untreated) cells displayed a typically scattered distribution of filamentary mitochondria inside the cell. After 2 hours of MEN treatment the spatial distribution of the mitochondria was markedly altered to an asymmetric perinuclear clustered distribution. Menadione-stressed cells displayed a highly asymmetrical perinuclear clustered distribution of the mitochondria. The exposure of cells to MEN also results in a change in shape of the mitochondria into a population of enlarged granular structures. The results of our study demonstrate that in JAR cells menadione causes mitochondria to translocate from the cell periphery into the perinuclear region several hours before disruption of cell membrane integrity and cell death.

Abstract

Mitochondrial dysfunction plays a crucial role in cell types that exhibit necrosislike death after activation of their death program. Tumour necrosis factor (TNF) induces abnormal, perinuclear clustering of mitochondria from an evenly spread distribution throughout the cytoplasm. The mitochondria withdraw from the cell periphery and aggregate in a unipolar perinuclear cluster. TNF-induced mitochondrial clustering is caused by impaired kinesin-mediated transportation of mitochondria. In this report, we describe a novel activity of menadione (MEN), namely the induction of an altered spatial distribution of mitochondria in the choriocarcinoma JAR cells. Strikingly, 2 hours of cell exposition to menadione did not disrupt the integrity of the plasma membrane, while the intracellular ATP level significantly decreased. Control (untreated) cells displayed a typically scattered distribution of filamentary mitochondria inside the cell. After 2 hours of MEN treatment the spatial distribution of the mitochondria was markedly altered to an asymmetric perinuclear clustered distribution. Menadione-stressed cells displayed a highly asymmetrical perinuclear clustered distribution of the mitochondria. The exposure of cells to MEN also results in a change in shape of the mitochondria into a population of enlarged granular structures. The results of our study demonstrate that in JAR cells menadione causes mitochondria to translocate from the cell periphery into the perinuclear region several hours before disruption of cell membrane integrity and cell death.
Get Citation

Keywords

JAR cells; menadione; TNF; oxidative stress; mitochondrial translocation

About this article
Title

Fast perinuclear clustering of mitochondria in oxidatively stressed human choriocarcinoma cells

Journal

Folia Morphologica

Issue

Vol 63, No 4 (2004)

Pages

407-412

Published online

2004-09-16

Bibliographic record

Folia Morphol 2004;63(4):407-412.

Keywords

JAR cells
menadione
TNF
oxidative stress
mitochondrial translocation

Authors

Anna Hallmann
Ryszard Milczarek
Marcin Lipiński
Ewa Kossowska
Jan Henryk Spodnik
Michał Woźniak
Takashi Wakabayashi
Jerzy Klimek

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl