Vol 63, No 4 (2004)
Original article
Published online: 2004-09-16

open access

Page views 1077
Article views/downloads 1661
Get Citation

Connect on Social Media

Connect on Social Media

Fast perinuclear clustering of mitochondria in oxidatively stressed human choriocarcinoma cells

Anna Hallmann, Ryszard Milczarek, Marcin Lipiński, Ewa Kossowska, Jan Henryk Spodnik, Michał Woźniak, Takashi Wakabayashi, Jerzy Klimek
Folia Morphol 2004;63(4):407-412.

Abstract

Mitochondrial dysfunction plays a crucial role in cell types that exhibit necrosislike death after activation of their death program. Tumour necrosis factor (TNF) induces abnormal, perinuclear clustering of mitochondria from an evenly spread distribution throughout the cytoplasm. The mitochondria withdraw from the cell periphery and aggregate in a unipolar perinuclear cluster. TNF-induced mitochondrial clustering is caused by impaired kinesin-mediated transportation of mitochondria. In this report, we describe a novel activity of menadione (MEN), namely the induction of an altered spatial distribution of mitochondria in the choriocarcinoma JAR cells. Strikingly, 2 hours of cell exposition to menadione did not disrupt the integrity of the plasma membrane, while the intracellular ATP level significantly decreased. Control (untreated) cells displayed a typically scattered distribution of filamentary mitochondria inside the cell. After 2 hours of MEN treatment the spatial distribution of the mitochondria was markedly altered to an asymmetric perinuclear clustered distribution. Menadione-stressed cells displayed a highly asymmetrical perinuclear clustered distribution of the mitochondria. The exposure of cells to MEN also results in a change in shape of the mitochondria into a population of enlarged granular structures. The results of our study demonstrate that in JAR cells menadione causes mitochondria to translocate from the cell periphery into the perinuclear region several hours before disruption of cell membrane integrity and cell death.

Article available in PDF format

View PDF Download PDF file