open access

Vol 63, No 4 (2004)
ORIGINAL ARTICLES
Published online: 2004-09-16
Submitted: 2012-02-06
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The influence of open field exposure on neurons containing nitric oxide synthase in the basolateral complex and paracapsular intercalated nerve cells of the rat amygdala

Beata Ludkiewicz, Ilona Klejbor, Beata Domaradzka-Pytel, Sławomir Wójcik, Anna Łuczyńska, Ewa Badowska-Szalewska, Jerzy Dziewiątkowski, Janusz Moryś
Folia Morphol 2004;63(4):381-385.

open access

Vol 63, No 4 (2004)
ORIGINAL ARTICLES
Published online: 2004-09-16
Submitted: 2012-02-06

Abstract

Our intention in the present study was to ascertain whether NO-producing cells in the basolateral complex (BLC) and paracapsular intercalated nerve cell groups (Ip) of the amygdala are activated in the open field (OF) test. The material consisted of 8 adult rat brains. The OF test was applied throughout 10 min and 90 min before the death of the animals. The brain sections were double stained using the antibodies against c-Fos (marker of neuronal activation) and against nitric oxide synthase (NOS — marker of NO-producing cells). The neurons containing NOS and those revealing c-Fos activity constituted distinct populations within both the BLC and Ip but NOS-immunoreactive fibres often surrounded the c-Fos-immunoreactive neurons. Our results suggest that (1) neurons of the basolateral complex of the amygdala and paracapsular intercalated islands are involved but probably not crucial for the open field stress response and (2) NOS-immunoreactive cells in the BLC and Ip are not activated after OF exposure.

Abstract

Our intention in the present study was to ascertain whether NO-producing cells in the basolateral complex (BLC) and paracapsular intercalated nerve cell groups (Ip) of the amygdala are activated in the open field (OF) test. The material consisted of 8 adult rat brains. The OF test was applied throughout 10 min and 90 min before the death of the animals. The brain sections were double stained using the antibodies against c-Fos (marker of neuronal activation) and against nitric oxide synthase (NOS — marker of NO-producing cells). The neurons containing NOS and those revealing c-Fos activity constituted distinct populations within both the BLC and Ip but NOS-immunoreactive fibres often surrounded the c-Fos-immunoreactive neurons. Our results suggest that (1) neurons of the basolateral complex of the amygdala and paracapsular intercalated islands are involved but probably not crucial for the open field stress response and (2) NOS-immunoreactive cells in the BLC and Ip are not activated after OF exposure.
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Keywords

stress; c-Fos protein; NO-producing neurons

About this article
Title

The influence of open field exposure on neurons containing nitric oxide synthase in the basolateral complex and paracapsular intercalated nerve cells of the rat amygdala

Journal

Folia Morphologica

Issue

Vol 63, No 4 (2004)

Pages

381-385

Published online

2004-09-16

Bibliographic record

Folia Morphol 2004;63(4):381-385.

Keywords

stress
c-Fos protein
NO-producing neurons

Authors

Beata Ludkiewicz
Ilona Klejbor
Beata Domaradzka-Pytel
Sławomir Wójcik
Anna Łuczyńska
Ewa Badowska-Szalewska
Jerzy Dziewiątkowski
Janusz Moryś

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