open access

Vol 63, No 4 (2004)
ORIGINAL ARTICLES
Published online: 2004-09-16
Submitted: 2012-02-06
Get Citation

Parvalbumin containing neurons of the piriform cortex in open field stress - a developmental study in the rat

Ewa Badowska-Szalewska, Ilona Klejbor, Beata Ludkiewicz, Anna Łuczyńska, Beata Domaradzka-Pytel, Jerzy Dziewiątkowski, Janusz Moryś
Folia Morphol 2004;63(4):367-372.

open access

Vol 63, No 4 (2004)
ORIGINAL ARTICLES
Published online: 2004-09-16
Submitted: 2012-02-06

Abstract

In our study we used c-Fos protein (as a marker of cellular activity) to identify whether cells containing parvalbumin (PV) in the piriform cortex (PC) are engaged in the response to stress stimulation and to discover how this expression changes during maturation. The material consisted of Wistar rats of postnatal (P) ages between 0 and 120 days divided into 9 groups: P0, P4, P7, P10, P14, P21, P30, P90, P120. Each group consisted of 5 experimental and 3 control animals. Rats of the experimental groups were exposed to the "open field test" throughout 10 minutes. The control animals were kept in a home cage. Our results showed that c-Fos activity in the open field test was observed in layers II and III of PC after birth. It then increased and stabilised on P30. In the second week of life PV-positive cells were also observed in those layers. These achieved maturity in the 4th week of life. After this time basket-like structures appeared but the level of PV/c-Fos co-localisation was low. Only small differences were observed between the anterior and posterior parts of PC. In the anterior part a higher number of PV-positive neurons, neuropil threads, and basket-like structures and a larger degree of PV/c-Fos co-localisation were observed. Our results suggested that during maturation PV cells are not directly activated in response to stress stimuli but PV neurons via their numerous endings influence the activation of c-Fos-positive cells predominantly in the anterior part of PC.

Abstract

In our study we used c-Fos protein (as a marker of cellular activity) to identify whether cells containing parvalbumin (PV) in the piriform cortex (PC) are engaged in the response to stress stimulation and to discover how this expression changes during maturation. The material consisted of Wistar rats of postnatal (P) ages between 0 and 120 days divided into 9 groups: P0, P4, P7, P10, P14, P21, P30, P90, P120. Each group consisted of 5 experimental and 3 control animals. Rats of the experimental groups were exposed to the "open field test" throughout 10 minutes. The control animals were kept in a home cage. Our results showed that c-Fos activity in the open field test was observed in layers II and III of PC after birth. It then increased and stabilised on P30. In the second week of life PV-positive cells were also observed in those layers. These achieved maturity in the 4th week of life. After this time basket-like structures appeared but the level of PV/c-Fos co-localisation was low. Only small differences were observed between the anterior and posterior parts of PC. In the anterior part a higher number of PV-positive neurons, neuropil threads, and basket-like structures and a larger degree of PV/c-Fos co-localisation were observed. Our results suggested that during maturation PV cells are not directly activated in response to stress stimuli but PV neurons via their numerous endings influence the activation of c-Fos-positive cells predominantly in the anterior part of PC.
Get Citation

Keywords

c-Fos; maturation; open field test

About this article
Title

Parvalbumin containing neurons of the piriform cortex in open field stress - a developmental study in the rat

Journal

Folia Morphologica

Issue

Vol 63, No 4 (2004)

Pages

367-372

Published online

2004-09-16

Bibliographic record

Folia Morphol 2004;63(4):367-372.

Keywords

c-Fos
maturation
open field test

Authors

Ewa Badowska-Szalewska
Ilona Klejbor
Beata Ludkiewicz
Anna Łuczyńska
Beata Domaradzka-Pytel
Jerzy Dziewiątkowski
Janusz Moryś

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl