open access

Vol 65, No 1 (2006)
Short Communications
Published online: 2005-12-05
Submitted: 2012-02-06
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The survival and proliferation of fibroblasts on orthodontic miniscrews with different surface treatment: an in vitro study

P. Proff, E. Kauschke, E. Rumpel, T. Bayerlein, S. Dietze, J. Fanghänel, T. Gedrange
Folia Morphol 2006;65(1):78-80.

open access

Vol 65, No 1 (2006)
Short Communications
Published online: 2005-12-05
Submitted: 2012-02-06

Abstract

It is of fundamental importance for prosthodontic and orthodontic applications that there is a short osseointegration time of dental implants without inflammation of the surrounding tissue. In addition to the chemical properties of the implant material, the surface morphology is an equally critical parameter. The objective of this work was to study the effect of two simple surface treatments on the survival and proliferation of fibroblasts.
Three groups of orthodontic miniscrews (Mondeal®) were used. One group was given an airflow (EMS, Schweiz) treatment, the second was sand-blasted in the area of the threading and a third group served as a control. After preparation sterilised screws were cultured in vitro with fibroblasts (L-929). The metabolic cell activity on the implant surface was determined after 24, 48 and 120 hours using the alamarBlue assay and a count of DAPI labelled fibroblasts was performed with a fluorescence microscope.
After 24 hours, but not at 48 hours and 120 hours, the metabolic activity of the fibroblasts was slightly decreased for the airflow screw group. Generally, no significant difference was found regarding metabolic activity and proliferation of fibroblasts within the different groups.

Abstract

It is of fundamental importance for prosthodontic and orthodontic applications that there is a short osseointegration time of dental implants without inflammation of the surrounding tissue. In addition to the chemical properties of the implant material, the surface morphology is an equally critical parameter. The objective of this work was to study the effect of two simple surface treatments on the survival and proliferation of fibroblasts.
Three groups of orthodontic miniscrews (Mondeal®) were used. One group was given an airflow (EMS, Schweiz) treatment, the second was sand-blasted in the area of the threading and a third group served as a control. After preparation sterilised screws were cultured in vitro with fibroblasts (L-929). The metabolic cell activity on the implant surface was determined after 24, 48 and 120 hours using the alamarBlue assay and a count of DAPI labelled fibroblasts was performed with a fluorescence microscope.
After 24 hours, but not at 48 hours and 120 hours, the metabolic activity of the fibroblasts was slightly decreased for the airflow screw group. Generally, no significant difference was found regarding metabolic activity and proliferation of fibroblasts within the different groups.
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Keywords

orthodontic miniscrews; surface treatment; cell culture; fibroblasts; surface colonisation

About this article
Title

The survival and proliferation of fibroblasts on orthodontic miniscrews with different surface treatment: an in vitro study

Journal

Folia Morphologica

Issue

Vol 65, No 1 (2006)

Pages

78-80

Published online

2005-12-05

Bibliographic record

Folia Morphol 2006;65(1):78-80.

Keywords

orthodontic miniscrews
surface treatment
cell culture
fibroblasts
surface colonisation

Authors

P. Proff
E. Kauschke
E. Rumpel
T. Bayerlein
S. Dietze
J. Fanghänel
T. Gedrange

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