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Vol 65, No 1 (2006)
Original article
Submitted: 2012-02-06
Published online: 2005-12-05
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The in vitro viability and growth of fibroblasts cultured in the presence of different bone grafting materials (NanoBone® and Straumann Bone Ceramic®)

E. Kauschke, E. Rumpel, J. Fanghänel, T. Bayerlein, T. Gedrange, P. Proff
Folia Morphol 2006;65(1):37-42.

open access

Vol 65, No 1 (2006)
ORIGINAL ARTICLES
Submitted: 2012-02-06
Published online: 2005-12-05

Abstract

Different clinical applications, including dentistry, are making increasing demands on bone grafting material. In the present study we have analysed the viability, proliferation and growth characteristics of fibroblasts cultured in vitro together with two different bone grafting materials, NanoBone® and Straumann Bone Ceramic®, over a period of 24 and 28 days respectively. Viability was measured at least every 72 hours by using the alamarBlue assay, a test that measures quantitatively cell proliferation and viability but does not require cell fixation or extraction. After one week of culture fibroblast viability was as high as in controls for both grafting materials and remained high (> 90%) for the duration of the experiment. Cell growth was evaluated microscopically. Scanning electron microscopy revealed a dense fibroblast growth at the surface of both bone grafting materials after three weeks of in vitro culture. Generally, our in vitro analyses contribute to further insights into cell - scaffold interactions.

Abstract

Different clinical applications, including dentistry, are making increasing demands on bone grafting material. In the present study we have analysed the viability, proliferation and growth characteristics of fibroblasts cultured in vitro together with two different bone grafting materials, NanoBone® and Straumann Bone Ceramic®, over a period of 24 and 28 days respectively. Viability was measured at least every 72 hours by using the alamarBlue assay, a test that measures quantitatively cell proliferation and viability but does not require cell fixation or extraction. After one week of culture fibroblast viability was as high as in controls for both grafting materials and remained high (> 90%) for the duration of the experiment. Cell growth was evaluated microscopically. Scanning electron microscopy revealed a dense fibroblast growth at the surface of both bone grafting materials after three weeks of in vitro culture. Generally, our in vitro analyses contribute to further insights into cell - scaffold interactions.

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Keywords

bone defects; bone remodelling; scanning electron microscopy; SEM; alamarBlue assay

About this article
Title

The in vitro viability and growth of fibroblasts cultured in the presence of different bone grafting materials (NanoBone® and Straumann Bone Ceramic®)

Journal

Folia Morphologica

Issue

Vol 65, No 1 (2006)

Article type

Original article

Pages

37-42

Published online

2005-12-05

Page views

468

Article views/downloads

1922

Bibliographic record

Folia Morphol 2006;65(1):37-42.

Keywords

bone defects
bone remodelling
scanning electron microscopy
SEM
alamarBlue assay

Authors

E. Kauschke
E. Rumpel
J. Fanghänel
T. Bayerlein
T. Gedrange
P. Proff

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