Vol 65, No 2 (2006)
Original article
Submitted: 2012-02-06
Published online: 2006-03-09
The effect of maternal diabetes on pancreatic islets in newborn rats: a quantitative and immunocytochemical study
M.H.M. Badawoud
Folia Morphol 2006;65(2):152-156.
Vol 65, No 2 (2006)
ORIGINAL ARTICLES
Submitted: 2012-02-06
Published online: 2006-03-09
Abstract
The effect of diabetes during pregnancy on the endocrine pancreas of the newborn
has been studied before, but a detailed morphometric immunocytochemical
study is not available. The aim of this study was to investigate the effect of
maternal diabetes on the morphology of islets in newborn rats. Pancreatic sections
were stained by the indirect immunoperoxidase method to localise the
insulin-producing β-cells and were used for morphometric analysis. The islet
volume density, diameter and volume and the absolute islet cell number were
significantly greater in the diabetic group than in controls. The β-cell volume
density was significantly lower in the diabetic group, although the β-cell nuclear
diameter and volume did not differ significantly in this group. The results obtained
from this investigation indicate that maternal diabetes induces islet hypertrophy in
newborn offspring and causes an increase in the total islet cell number.
Abstract
The effect of diabetes during pregnancy on the endocrine pancreas of the newborn
has been studied before, but a detailed morphometric immunocytochemical
study is not available. The aim of this study was to investigate the effect of
maternal diabetes on the morphology of islets in newborn rats. Pancreatic sections
were stained by the indirect immunoperoxidase method to localise the
insulin-producing β-cells and were used for morphometric analysis. The islet
volume density, diameter and volume and the absolute islet cell number were
significantly greater in the diabetic group than in controls. The β-cell volume
density was significantly lower in the diabetic group, although the β-cell nuclear
diameter and volume did not differ significantly in this group. The results obtained
from this investigation indicate that maternal diabetes induces islet hypertrophy in
newborn offspring and causes an increase in the total islet cell number.
Keywords
quantitative; β-cells; immunocytochemical
Title
The effect of maternal diabetes on pancreatic islets in newborn rats: a quantitative and immunocytochemical study
Journal
Folia Morphologica
Issue
Vol 65, No 2 (2006)
Article type
Original article
Pages
152-156
Published online
2006-03-09
Page views
439
Article views/downloads
1558
Bibliographic record
Folia Morphol 2006;65(2):152-156.
Keywords
quantitative
β-cells
immunocytochemical