Vol 65, No 4 (2006)
Original article
Submitted: 2012-02-06
Published online: 2006-09-18
Fluoride alters type I collagen expression in the early stages of odontogenesis
Maciejewska I, Spodnik JH, Domaradzka-Pytel B, Sidor-Kaczmarek J, Bereznowski Z
Folia Morphol 2006;65(4):359-366.
Vol 65, No 4 (2006)
ORIGINAL ARTICLES
Submitted: 2012-02-06
Published online: 2006-09-18
Abstract
Fluoride alters the expression and post-translational modifications of extracellular
matrix proteins in dentin. The aim of our study was to determine the effects
of fluoride on type I collagen expression during the early stages of tooth germ
development in rats. Pregnant dams were divided into three groups and fed
a standard diet. From the fifth day of pregnancy the three groups received tap
water with, respectively, trace amounts of fluoride (C), a low fluoride concentration
(FL) or and a high fluoride concentration (FH). Changes in type I collagen
expression and distribution were evaluated. The expression of type I collagen
was restricted to the extracellular spaces of cells of mesenchymal origin. In the
youngest animals the most intense immunoreactivity for type I collagen was
detected in predentin of the FL group. Although the intensity of immunostaining
increased in proportion to the age of the animals, the largest increase in the
groups investigated was detected in the FL group. We concluded that a low
concentration of fluoride can act as a stimulator of type I collagen deposition in
the extracellular matrix of dentin, while high concentrations of fluoride have an
opposite effect, acting as an inhibitor of type I collagen formation in dentin.
Abstract
Fluoride alters the expression and post-translational modifications of extracellular
matrix proteins in dentin. The aim of our study was to determine the effects
of fluoride on type I collagen expression during the early stages of tooth germ
development in rats. Pregnant dams were divided into three groups and fed
a standard diet. From the fifth day of pregnancy the three groups received tap
water with, respectively, trace amounts of fluoride (C), a low fluoride concentration
(FL) or and a high fluoride concentration (FH). Changes in type I collagen
expression and distribution were evaluated. The expression of type I collagen
was restricted to the extracellular spaces of cells of mesenchymal origin. In the
youngest animals the most intense immunoreactivity for type I collagen was
detected in predentin of the FL group. Although the intensity of immunostaining
increased in proportion to the age of the animals, the largest increase in the
groups investigated was detected in the FL group. We concluded that a low
concentration of fluoride can act as a stimulator of type I collagen deposition in
the extracellular matrix of dentin, while high concentrations of fluoride have an
opposite effect, acting as an inhibitor of type I collagen formation in dentin.
Keywords
dentin; tooth germ development; rat
Title
Fluoride alters type I collagen expression in the early stages of odontogenesis
Journal
Folia Morphologica
Issue
Vol 65, No 4 (2006)
Article type
Original article
Pages
359-366
Published online
2006-09-18
Page views
534
Article views/downloads
1270
Bibliographic record
Folia Morphol 2006;65(4):359-366.
Keywords
dentin
tooth germ development
rat
Authors
Maciejewska I
Spodnik JH
Domaradzka-Pytel B
Sidor-Kaczmarek J
Bereznowski Z