open access

Vol 65, No 4 (2006)
ORIGINAL ARTICLES
Published online: 2006-09-18
Submitted: 2012-02-06
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Fluoride alters type I collagen expression in the early stages of odontogenesis

Maciejewska I, Spodnik JH, Domaradzka-Pytel B, Sidor-Kaczmarek J, Bereznowski Z
Folia Morphol 2006;65(4):359-366.

open access

Vol 65, No 4 (2006)
ORIGINAL ARTICLES
Published online: 2006-09-18
Submitted: 2012-02-06

Abstract

Fluoride alters the expression and post-translational modifications of extracellular matrix proteins in dentin. The aim of our study was to determine the effects of fluoride on type I collagen expression during the early stages of tooth germ development in rats. Pregnant dams were divided into three groups and fed a standard diet. From the fifth day of pregnancy the three groups received tap water with, respectively, trace amounts of fluoride (C), a low fluoride concentration (FL) or and a high fluoride concentration (FH). Changes in type I collagen expression and distribution were evaluated. The expression of type I collagen was restricted to the extracellular spaces of cells of mesenchymal origin. In the youngest animals the most intense immunoreactivity for type I collagen was detected in predentin of the FL group. Although the intensity of immunostaining increased in proportion to the age of the animals, the largest increase in the groups investigated was detected in the FL group. We concluded that a low concentration of fluoride can act as a stimulator of type I collagen deposition in the extracellular matrix of dentin, while high concentrations of fluoride have an opposite effect, acting as an inhibitor of type I collagen formation in dentin.

Abstract

Fluoride alters the expression and post-translational modifications of extracellular matrix proteins in dentin. The aim of our study was to determine the effects of fluoride on type I collagen expression during the early stages of tooth germ development in rats. Pregnant dams were divided into three groups and fed a standard diet. From the fifth day of pregnancy the three groups received tap water with, respectively, trace amounts of fluoride (C), a low fluoride concentration (FL) or and a high fluoride concentration (FH). Changes in type I collagen expression and distribution were evaluated. The expression of type I collagen was restricted to the extracellular spaces of cells of mesenchymal origin. In the youngest animals the most intense immunoreactivity for type I collagen was detected in predentin of the FL group. Although the intensity of immunostaining increased in proportion to the age of the animals, the largest increase in the groups investigated was detected in the FL group. We concluded that a low concentration of fluoride can act as a stimulator of type I collagen deposition in the extracellular matrix of dentin, while high concentrations of fluoride have an opposite effect, acting as an inhibitor of type I collagen formation in dentin.
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Keywords

dentin; tooth germ development; rat

About this article
Title

Fluoride alters type I collagen expression in the early stages of odontogenesis

Journal

Folia Morphologica

Issue

Vol 65, No 4 (2006)

Pages

359-366

Published online

2006-09-18

Bibliographic record

Folia Morphol 2006;65(4):359-366.

Keywords

dentin
tooth germ development
rat

Authors

Maciejewska I
Spodnik JH
Domaradzka-Pytel B
Sidor-Kaczmarek J
Bereznowski Z

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