open access

Vol 68, No 2 (2009)
ORIGINAL ARTICLES
Published online: 2009-02-25
Submitted: 2012-02-06
Get Citation

The effect of Urtica dioica extract on the number of astrocytes in the dentate gyrus of diabetic rats

M. Jahanshahi, M.J. Golalipour, M. Afshar
Folia Morphol 2009;68(2):93-97.

open access

Vol 68, No 2 (2009)
ORIGINAL ARTICLES
Published online: 2009-02-25
Submitted: 2012-02-06

Abstract

Diabetes mellitus is associated with cerebral alterations in both human and animal models of the disease. These alterations include abnormal expression of hypothalamic neuropeptides and hippocampal astrogliosis. Urtica dioica (Nettle) is among several species listed for their use against diabetes in folk medicine. The aim of this study was the evaluation of the astrocyte number in the dentate gyrus of diabetic rats after treatment with nettle.
A total of 21 male albino Wistar rats were used in the present study. The animals were divided into three groups: control, nettle-untreated diabetic, and nettle treated diabetic. Hyperglycaemia was induced by streptozotocin (80 mg/kg) in the animals of the diabetic and treatment groups. One week after injection of the streptozotocin, the animals in the treatment group received a hydroalcoholic extract of Urtica dioica (100 mg/kg/day) for 4 weeks intraperitoneally. After a 5-week survival period, all the rats were sacrificed and coronal sections were taken from the dorsal hippocampal formation of the right cerebral hemispheres. The area densities of the astrocytes were measured and compared between the three groups (p < 0.05).
The number of astrocytes increased in the diabetic rats (24.06 ± 9.57) compared with the controls (17.52 ± 6.66). The densities in the treated rats (19.50 ± 6.16) were lower than in the diabetic rats. Furthermore, the control and treated rats showed similar densities.
We concluded that U. dioica extract helped compensate for astrocytes in the treatment rats dentate gyrus in comparison with diabetic rats.

Abstract

Diabetes mellitus is associated with cerebral alterations in both human and animal models of the disease. These alterations include abnormal expression of hypothalamic neuropeptides and hippocampal astrogliosis. Urtica dioica (Nettle) is among several species listed for their use against diabetes in folk medicine. The aim of this study was the evaluation of the astrocyte number in the dentate gyrus of diabetic rats after treatment with nettle.
A total of 21 male albino Wistar rats were used in the present study. The animals were divided into three groups: control, nettle-untreated diabetic, and nettle treated diabetic. Hyperglycaemia was induced by streptozotocin (80 mg/kg) in the animals of the diabetic and treatment groups. One week after injection of the streptozotocin, the animals in the treatment group received a hydroalcoholic extract of Urtica dioica (100 mg/kg/day) for 4 weeks intraperitoneally. After a 5-week survival period, all the rats were sacrificed and coronal sections were taken from the dorsal hippocampal formation of the right cerebral hemispheres. The area densities of the astrocytes were measured and compared between the three groups (p < 0.05).
The number of astrocytes increased in the diabetic rats (24.06 ± 9.57) compared with the controls (17.52 ± 6.66). The densities in the treated rats (19.50 ± 6.16) were lower than in the diabetic rats. Furthermore, the control and treated rats showed similar densities.
We concluded that U. dioica extract helped compensate for astrocytes in the treatment rats dentate gyrus in comparison with diabetic rats.
Get Citation

Keywords

diabetes; astrocyte; dentate gyrus; Urtica dioica

About this article
Title

The effect of Urtica dioica extract on the number of astrocytes in the dentate gyrus of diabetic rats

Journal

Folia Morphologica

Issue

Vol 68, No 2 (2009)

Pages

93-97

Published online

2009-02-25

Bibliographic record

Folia Morphol 2009;68(2):93-97.

Keywords

diabetes
astrocyte
dentate gyrus
Urtica dioica

Authors

M. Jahanshahi
M.J. Golalipour
M. Afshar

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl