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Simvastatin attenuates intestinal ischaemia/reperfusion-induced injury in rat
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Abstract
Thirty-six male wistar rats underwent laparotomy under general anaesthesia. Simvastatin was administered from four days before ischaemia induction. The rats were divided in to three groups (n = 12): the sham goup, the I/R group, and the I/R + simvastatin group. Intestinal ischaemia was induced by superior mesenteric artery ligation with microvascular clamps for 60 minutes, and after ischaemia, blood perfusion was released into the tissue and a reperfusion phase was started, which lasted for 3 hours. After 3 hours, the animals were sacrificed and serum and tissue obtained for biochemical and histological study. In the simvastatin treated group, intestinal tissue injury, TNF-α level, and tissue malondealdehyde levels were significantly lower than in the I/R group (p < 0.05). Glutathion peroxidase and superoxide dismutase levels were significantly higher in the simvastatin treated group than in the I/R group (p < 0.05).
Simvastatin pretreatment reduced intestinal I/R injury and was associated with down- -regulation of serum TNF-α and tissue malondealdehyde level, and simvastatin administration maintained cellular antioxidant enzyme contents compared to the I/R group after 3 hours reperfusion time.
Abstract
Thirty-six male wistar rats underwent laparotomy under general anaesthesia. Simvastatin was administered from four days before ischaemia induction. The rats were divided in to three groups (n = 12): the sham goup, the I/R group, and the I/R + simvastatin group. Intestinal ischaemia was induced by superior mesenteric artery ligation with microvascular clamps for 60 minutes, and after ischaemia, blood perfusion was released into the tissue and a reperfusion phase was started, which lasted for 3 hours. After 3 hours, the animals were sacrificed and serum and tissue obtained for biochemical and histological study. In the simvastatin treated group, intestinal tissue injury, TNF-α level, and tissue malondealdehyde levels were significantly lower than in the I/R group (p < 0.05). Glutathion peroxidase and superoxide dismutase levels were significantly higher in the simvastatin treated group than in the I/R group (p < 0.05).
Simvastatin pretreatment reduced intestinal I/R injury and was associated with down- -regulation of serum TNF-α and tissue malondealdehyde level, and simvastatin administration maintained cellular antioxidant enzyme contents compared to the I/R group after 3 hours reperfusion time.
Keywords
simvastatin-Intestine; ischaemia/reperfusion; injury
Title
Simvastatin attenuates intestinal ischaemia/reperfusion-induced injury in rat
Journal
Issue
Article type
Original article
Pages
156-162
Published online
2009-05-13
Page views
467
Article views/downloads
1276
Bibliographic record
Folia Morphol 2009;68(3):156-162.
Keywords
simvastatin-Intestine
ischaemia/reperfusion
injury
Authors
B. Hajipour
M.H. Somi
F. Saberifar
M.R. Hemmati
N.A. Asl
A. Moein
A.M. Vatankhah
A.R. Nourazar
M.R. Nasirizade