open access

Vol 68, No 3 (2009)
ORIGINAL ARTICLES
Published online: 2009-05-13
Submitted: 2012-02-06
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Changes in NGF/c-Fos colocalization in specific limbic structures of juvenile and aged rats after open field stimulation

E. Badowska-Szalewska, I. Klejbor, T. Cecot, B. Domaradzka-Pytel, B. Ludkiewicz, J. Moryś
Folia Morphol 2009;68(3):129-134.

open access

Vol 68, No 3 (2009)
ORIGINAL ARTICLES
Published online: 2009-05-13
Submitted: 2012-02-06

Abstract

Changes in NGF release during stressful events have been associated with the activation of neurons expressing NGF receptors. This study examined the influence of acute stress-induced stimulation on NGF/c-Fos colocalization in the following limbic regions: the paraventricular (PV) nucleus of the hypothalamus, medial (MeA) nucleus of the amygdala, and CA3 hippocampus. Juvenile (P21) and aged rats (P360) were exposed to a 15-minute acute open field (OF) test. Double immunofluorescence staining, used to detect NGF-ir and c-Fos-ir cells, revealed a higher percentage of NGF/c-Fos-ir neurons in the P21 control group than in the P360 control group. Under OF acute stimulation, a statistically significant (p < 0.05) increase of NGF/c-Fos level in CA3 of juvenile animals and in PV and CA3 of the aged rats was observed. These observations indicate that the investigated structures in both age groups show a different response to acute OF stimulation. Acute OF affects the levels of NGF/c-Fos more significantly in aged rats.

Abstract

Changes in NGF release during stressful events have been associated with the activation of neurons expressing NGF receptors. This study examined the influence of acute stress-induced stimulation on NGF/c-Fos colocalization in the following limbic regions: the paraventricular (PV) nucleus of the hypothalamus, medial (MeA) nucleus of the amygdala, and CA3 hippocampus. Juvenile (P21) and aged rats (P360) were exposed to a 15-minute acute open field (OF) test. Double immunofluorescence staining, used to detect NGF-ir and c-Fos-ir cells, revealed a higher percentage of NGF/c-Fos-ir neurons in the P21 control group than in the P360 control group. Under OF acute stimulation, a statistically significant (p < 0.05) increase of NGF/c-Fos level in CA3 of juvenile animals and in PV and CA3 of the aged rats was observed. These observations indicate that the investigated structures in both age groups show a different response to acute OF stimulation. Acute OF affects the levels of NGF/c-Fos more significantly in aged rats.
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Keywords

paraventricular nucleus; medial amygdaloid nucleus; CA3; limbic system; open field; stress

About this article
Title

Changes in NGF/c-Fos colocalization in specific limbic structures of juvenile and aged rats after open field stimulation

Journal

Folia Morphologica

Issue

Vol 68, No 3 (2009)

Pages

129-134

Published online

2009-05-13

Bibliographic record

Folia Morphol 2009;68(3):129-134.

Keywords

paraventricular nucleus
medial amygdaloid nucleus
CA3
limbic system
open field
stress

Authors

E. Badowska-Szalewska
I. Klejbor
T. Cecot
B. Domaradzka-Pytel
B. Ludkiewicz
J. Moryś

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