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Published online: 2024-06-11

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A new corrosion method (Aycan’s method)

Kenan Aycan1, Fatma Köse2, Burcu Kamaşak Arpaçay1, Tufan Ulcay1, Ayhan Düzler3
DOI: 10.5603/fm.100364


Background: As it is known, the anatomy of the vessels is examined by removing the cast of the vessels inside the organs. Generally, liquid material (polyester, takilon, etc.) is injected into the vessels with positive pressure to remove the cast from the vessels of the organs. We built a machine to remove the cast of the vessels inside the organ. We named it corrosion machine.

Materials and methods: Sheep kidneys were used in our experiment. After the kidneys were cannulated, they were placed in the vacuum chamber. With the operation of the vacuum pump, negative pressure was created in the vacuum chamber. With negative pressure, kidneys and its vessels expanded. Takilon or polyester easily entered the vessels of the kidney. The cast of the vessels of the kidney was removed. With this newly developed technique, the anatomy of the vessels whose casts were removed was examined with the naked eye, stereomicroscope or SEM.

Results: The corrosion machine we built can cast the vessels of the organs very well. Takilon or polyester (which we used in our experiment) easily entered the capillaries under the effect of negative pressure.

Conclusions: We think that this method can also be applied to other organs and used in vascular research.

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  1. Aycan K, Ulcay T, Kamaşak B. The morphology of the afferent and efferent domain of the sheep glomerulus. Folia Morphol. 2021; 80(4): 881–887.
  2. Aydinlik E, Aycan K, Ekinci N. Anatomy of the coronary veins in akkaraman sheep. J Health Sci. 2000; 17(1): 40–45.
  3. Bordei P, St Antohe D, Sapte E, et al. Morphological aspects of the inferior suprarenal artery. Surg Radiol Anat. 2003; 25(3-4): 247–251.
  4. Boron WF. Organisation of the Respiratory System. In: Boron WF, Boulpaep EL. ed. Medical Physiology. 3rd ed. Elsevier : 590–700.
  5. Brown JA. Renal microvasculature of the rainbow trout, Salmo gairdneri: scanning electron microscopy of corrosion casts of glomeruli. Anat Rec. 1985; 213(4): 505–513.
  6. Casotti G, Braun EJ. Structure of the glomerular capillaries of the domestic chicken and desert quail. J Morphol. 1995; 224(1): 57–63.
  7. De Sordi N, Bombardi C, Chiocchetti R, et al. A new method of producing casts for anatomical studies. Anat Sci Int. 2014; 89(4): 255–265.
  8. Domagała Z, Domański J, Smyczek N, et al. Maceration stage in corrosion cast specimen procedure in anatomy: a minireview. Folia Morphol. 2022; 81(4): 825–833.
  9. Düzler A, Nur İH, Alan AA. Macroanatomical study on ramification and course of aorta descendens in japanese quail. J Fac Vet Med Univ. 2011; 8(3): 139–152.
  10. Elger M, Sakai T, Kriz W. The vascular pole of the renal glomerulus of rat. Adv Anat Embryol Cell Biol. 1998; 139: 1–98.
  11. Ilić M, Milisavljević M, Maliković A, et al. The superficial palmar branch of the radial artery: a corrosion cast study. Folia Morphol. 2018; 77(4): 649–655.
  12. Kłosińska D, Ciszek B, Majchrzak B, et al. Diversity of coronary arterial tree in laboratory mice. Folia Morphol. 2020; 79(2): 255–264.
  13. Kose F, Duzler A. An anatomical and volumetric study on brain ventricles in sheep using different techniques. Anat Histol Embryol. 2023; 52(5): 732–741.
  14. Maga P, Tomaszewski KA, Skrzat J, et al. Microanatomical study of the recurrent artery of Heubner. Ann Anat. 2013; 195(4): 342–350.
  15. Narayanan VR. Preparation of low cost bronchopulmonary airway cast. J Anat Soc India. 2015; 64(2): 162–165.
  16. Nerantzis C, Antonakis E, Avgoustakis D. A new corrosion casting technique. Anat Rec. 1978; 191(3): 321–325.
  17. Oguz O, Dere F, Yücel AH, et al. Examination of microvascular structures of midcortical region in sheep kidneys: a three dimensional approach. Acta Med Okayama. 1991; 45(2): 77–80.
  18. Oguz O, Dere F, Yücel AH, et al. Scanning electron microscopic (SEM) examination of structures which supply subcapsular and midcortical region postglomerular microcirculation in sheep kidney. Acta Morphol Hung. 1992; 40(1-4): 195–202.
  19. Shanthini S, Suma HY. An innovative method in venous coronary cast technique. Anat Cell Biol. 2019; 52(2): 191–195.