Investigation of the effectiveness of atmospheric pressure cold plasma on sciatic nerve injury in rats
Abstract
Background: The aim of this study was to evaluate the efficacy of atmospheric pressure cold plasma jet and plasma activated medium (PAM) on sciatic nerve injury (SNI).
Materials and methods: Rats were divided into 6 groups (n = 10); group 1 (Sham), group 2 (SNI), group 3 (SNI + Atmospheric pressure cold plasma jet 5 min), group 4 (SNI + Atmospheric pressure cold plasma jet 10 min), group 5 (SNI + PAM 5 min), group 6 (SNI + PAM 10 min). On the 1st, 8th, 15th, 22nd days of the study, atmospheric pressure cold plasma jet was applied to rats in groups 3 and 4, and PAM was applied to rats in groups 5 and 6. Hot plate test was applied to all rats on the same days. On day 28, the experiment was terminated and sciatic nerve tissues were removed for histopathologic evaluations.
Results: According to the 4-week average of the hot plate tests, a significant relationship was found between group 2 and group 4 and group 6 (p < 0.05). When evaluated within each week, significant differences were found between group 2 and group 4 in week 1, between group 2 and group 5 and group 6 in week 2, between group 2 and group 4 in week 3, and between group 2 and group 4 and group 6 in week 4 (p < 0.05). As a result of histopathologic analysis, except for the control group, the other groups had similar characteristics in terms of axonal degeneration, periaxonal swelling and axon density.
Conclusions: As a result of our study, we found that plasma application showed an improvement in the duration of the hot plate test, but did not show any improvement histopathologically.
Keywords: atmospheric pressure cold plasma jetplasma-activated mediasciatic injuryhot plate testhistopathology
References
- An Y, Yan HX, Zhao JN, et al. Evaluation methods of a rat sciatic nerve crush injury model. J Integr Neurosci. 2022; 21(3): 91.
- Attri P, Park JiH, Ali A, et al. How does plasma activated media treatment differ from direct cold plasma treatment? Anticancer Agents Med Chem. 2018; 18(6): 805–814.
- Babaeva N, Kushner M. Reactive fluxes delivered by dielectric barrier discharge filaments to slightly wounded skin. J Phys D: Appl Phys. 2012; 46(2): 025401.
- Boeckmann L, Bernhardt T, Schäfer M, et al. Plasma medicine: applications of cold atmospheric pressure plasma in dermatology. Oxid Med Cell Longev. 2019; 2019(2): 3873928–113.
- Canatan F. Atmosferik basınç plazma ile ilaç tedavisinin meme kanseri hücreleri üzerine etkilerinin karşılaştırılması: Lisansüstü Eğitim Enstitüsü.
- Cha S, Park YS. Plasma in dentistry. Clin Plasma Med. 2014; 2(1): 4–10.
- Cheng YJ, Lin CK, Chen CY, et al. Plasma-activated medium as adjuvant therapy for lung cancer with malignant pleural effusion. Sci Rep. 2020; 10(1): 18154.
- Durak MA, Ozhan O, Tetik B, et al. Effects of apocynin on sciatic nerve injury in rabbits. Biotech Histochem. 2023; 98(3): 172–178.
- Ermolaeva SA, Varfolomeev AF, Chernukha MYu, et al. Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds. J Med Microbiol. 2011; 60(Pt 1): 75–83.
- Gan Lu, Duan J, Zhang S, et al. Cold atmospheric plasma ameliorates imiquimod-induced psoriasiform dermatitis in mice by mediating antiproliferative effects. Free Radic Res. 2019; 53(3): 269–280.
- He R, Li Q, Shen W, et al. The efficacy and safety of cold atmospheric plasma as a novel therapy for diabetic wound in vitro and in vivo. Int Wound J. 2020; 17(3): 851–863.
- Heinlin J, Morfill G, Landthaler M, et al. Plasma medicine: possible applications in dermatology. J Dtsch Dermatol Ges. 2010; 8(12): 968–977.
- Henry JL. Mechanisms of peripheral nerve injury – what to treat, when to treat. IntechOpen, London 2014.
- Hoffmann C, Berganza C, Zhang J. Cold Atmospheric Plasma: methods of production and application in dentistry and oncology. Med Gas Res. 2013; 3(1): 21.
- Isbary G, Shimizu T, Li YF, et al. Cold atmospheric plasma devices for medical issues. Expert Rev Med Devices. 2013; 10(3): 367–377.
- Iwahashi T, Suzuki K, Tanaka H, et al. Neurotropin® accelerates peripheral nerve regeneration in a rat sciatic nerve crush injury model. J Orthop Sci. 2024; 29(2): 653–659.
- Keidar M, Walk R, Shashurin A, et al. Cold plasma selectivity and the possibility of a paradigm shift in cancer therapy. Br J Cancer. 2011; 105(9): 1295–1301.
- Kim YJ, Lim DJ, Lee MiY, et al. Prospective, comparative clinical pilot study of cold atmospheric plasma device in the treatment of atopic dermatitis. Sci Rep. 2021; 11(1): 14461.
- Korkmaz MF, Parlakpinar H, Erdem MN, et al. The therapeutic efficacy of dexpanthenol on sciatic nerve injury in a rat model. Br J Neurosurg. 2020; 34(4): 397–401.
- Laroussi M. Nonthermal decontamination of biological media by atmospheric-pressure plasmas: review, analysis, and prospects. IEEE Trans Plasma Sci. 2002; 30(4): 1409–1415.
- Laroussi M, Mohades S, Barekzi N. Killing adherent and nonadherent cancer cells with the plasma pencil. Biointerphases. 2015; 10(2).
- Lee HG, Choi JH, Jang YS, et al. Non-thermal plasma accelerates the healing process of peripheral nerve crush injury in rats. Int J Med Sci. 2020; 17(8): 1112–1120.
- Lee ST, Jang YS, Kim UK, et al. Non-thermal plasma application enhances the recovery of transected sciatic nerves in rats. Exp Biol Med (Maywood). 2021; 246(11): 1287–1296.
- Li M, Gao J, Wang L, et al. Basic research and clinical exploration of cold atmospheric plasma for skin wounds. Bioeng Transl Med. 2023; 8(5): e10550.
- Liu J, Yang C, Cheng C, et al. In vitro antimicrobial effect and mechanism of action of plasma-activated liquid on planktonic . Bioengineered. 2021; 12(1): 4605–4619.
- Maho T, Binois R, Brulé-Morabito F, et al. Anti-Bacterial action of plasma multi-jets in the context of chronic wound healing. Applied Sciences. 2021; 11(20): 9598.
- Moisan M, Barbeau J, Crevier MC, et al. Plasma sterilization. Methods and mechanisms. Pure Appl Chem. 2009; 74(3): 349–358.
- Ogut E, Yildirim FB, Sarikcioglu L, et al. Neuroprotective effects of ozone therapy after sciatic nerve cut injury. Kurume Med J. 2020; 65(4): 137–144.
- Ozdemir E, Gursoy S, Bagcivan I. The effects of serotonin/norepinephrine reuptake inhibitors and serotonin receptor agonist on morphine analgesia and tolerance in rats. J Physiol Sci. 2012; 62(4): 317–323.
- Özdemir A. Soğuk Atmosferik Plazma ve Kanser. Researcher. 2021; 1(2): 6–18.
- Ozhan O, Izci SF, Huz M, et al. Therapeutic effects of cinnamon bark oil on sciatic nerve injury in rats. Eur Rev Med Pharmacol Sci. 2023; 27(12): 5841–5853.
- Oztan MO, Ercan UK, Aksoy Gokmen A, et al. Irrigation of peritoneal cavity with cold atmospheric plasma treated solution effectively reduces microbial load in rat acute peritonitis model. Sci Rep. 2022; 12(1): 3646.
- Rasouli M, Mehdian H, Hajisharifi K, et al. Plasma activated medium induces apoptosis in chemotherapy-resistant ovarian cancer cells: high selectivity and synergy with carboplatin. Plasma Processes and Polymers. 2021; 18(9).
- Rodríguez F, Valero-Cabré A, Navarro X. Regeneration and functional recovery following peripheral nerve injury. Drug Discov Today: Dis Models. 2004; 1(2): 177–185.
- Salehi M, Naseri-Nosar M, Ebrahimi-Barough S, et al. Regeneration of sciatic nerve crush injury by a hydroxyapatite nanoparticle-containing collagen type I hydrogel. J Physiol Sci. 2018; 68(5): 579–587.
- Scholtz V, Pazlarova J, Souskova H, et al. Nonthermal plasma — a tool for decontamination and disinfection. Biotechnol Adv. 2015; 33(6 Pt 2): 1108–1119.
- Tendero C, Tixier C, Tristant P, et al. Atmospheric pressure plasmas: A review. Spectrochim Acta Part B At Spectrosc. 2006; 61(1): 2–30.
- Tušek L, Nitschke M, Werner C, et al. Surface characterisation of NH3 plasma treated polyamide 6 foils. Colloids Surf A: Physicochem Eng Asp. 2001; 195(1-3): 81–95.
- Vecchio D, Dai T, Huang L, et al. Antimicrobial photodynamic therapy with RLP068 kills methicillin-resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion. J Biophotonics. 2013; 6(9): 733–742.
- Xiao H, Wei C, Liu H, et al. Lentinan alleviates sciatic nerve injury by promoting autophagy to remove myelin fragments. Phytother Res. 2023; 37(9): 4042–4058.
