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Vol 60, No 3 (2022)
Original paper
Submitted: 2022-01-22
Accepted: 2022-07-19
Published online: 2022-08-04
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Activation of the JAK1/STAT1 signaling pathway is associated with peroxiredoxin 6 expression levels in human epididymis epithelial cells

Hui Shi1, Xiaoyu Liu2, Yanwei Wang3, Haiyan Wang3, Bochen Pan2, Jianyuan Li4
DOI: 10.5603/FHC.a2022.0021
·
Pubmed: 35929062
·
Folia Histochem Cytobiol 2022;60(3):226-236.
Affiliations
  1. College of Life Science, Yantai University, Yantai, Shandong Province, PR China
  2. Center of Reproductive Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning Province, PR China
  3. Qingdao Medical College Affiliated Hospital of Yantai Yuhuangding Hospital, Yantai, Shandong Province, PR China
  4. Key Laboratory of Male Reproductive Health, National Health and Family Planning Commission, Beijing, PR China

open access

Vol 60, No 3 (2022)
ORIGINAL PAPERS
Submitted: 2022-01-22
Accepted: 2022-07-19
Published online: 2022-08-04

Abstract

Introduction. Peroxiredoxin 6 (Prdx6) is widely expressed in mammalian tissues. Our previous study demonstrated that Prdx6 was expressed in human epididymis, present in human seminal fluid, and in spermatozoa. The protective role of Prdx6 in maintaining the viability and DNA integrity of human spermatozoa was also detected. Here, we demonstrate the potential role and mechanism of Prdx6 in human epididymis epithelial cells (HEECs).
Material and methods. Western blotting was used to measure expression levels of key proteins in the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway. The malonaldehyde (MDA) levels and antioxidant capacity in HEECs were detected with the commercial kits. Digital gene expression analysis (DGE) was used to identify gene expression patterns in control and Prdx6-interference HEECs. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to validate the DGE findings.
Results. Compared to control HEECs, the expression levels of JAK1, STAT1, phosphorylated JAK1 and STAT1 were significantly increased, while the expression level of SOCS3 was significantly decreased in Prdx6-interference HEECs. The MDA level and total antioxidant capacity in Prdx6-interference HEECs were significantly increased and decreased compared to that of control, respectively. DGE analysis identified 589 up-regulated and 314 down-regulated genes (including Prdx6) in Prdx6-interference HEECs. Thirteen significantly different pathways were identified between the two groups, with the majority of genes belonging to the CCL, CXCL, IL, and IFIT family of proteins and were related to immunity. In particular, the expression levels of IL6, IL6ST, and eighteen IFN-related genes were significantly increased in Prdx6-interference HEECs compared to control HEECs.
Conclusions. We found that reduced Prdx6 expression induced higher ROS levels in HEECs, which resulted in the activation of the IL-6 receptor and IFNγ expression to induce the JAK1/STAT1 signaling pathway.

Abstract

Introduction. Peroxiredoxin 6 (Prdx6) is widely expressed in mammalian tissues. Our previous study demonstrated that Prdx6 was expressed in human epididymis, present in human seminal fluid, and in spermatozoa. The protective role of Prdx6 in maintaining the viability and DNA integrity of human spermatozoa was also detected. Here, we demonstrate the potential role and mechanism of Prdx6 in human epididymis epithelial cells (HEECs).
Material and methods. Western blotting was used to measure expression levels of key proteins in the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway. The malonaldehyde (MDA) levels and antioxidant capacity in HEECs were detected with the commercial kits. Digital gene expression analysis (DGE) was used to identify gene expression patterns in control and Prdx6-interference HEECs. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to validate the DGE findings.
Results. Compared to control HEECs, the expression levels of JAK1, STAT1, phosphorylated JAK1 and STAT1 were significantly increased, while the expression level of SOCS3 was significantly decreased in Prdx6-interference HEECs. The MDA level and total antioxidant capacity in Prdx6-interference HEECs were significantly increased and decreased compared to that of control, respectively. DGE analysis identified 589 up-regulated and 314 down-regulated genes (including Prdx6) in Prdx6-interference HEECs. Thirteen significantly different pathways were identified between the two groups, with the majority of genes belonging to the CCL, CXCL, IL, and IFIT family of proteins and were related to immunity. In particular, the expression levels of IL6, IL6ST, and eighteen IFN-related genes were significantly increased in Prdx6-interference HEECs compared to control HEECs.
Conclusions. We found that reduced Prdx6 expression induced higher ROS levels in HEECs, which resulted in the activation of the IL-6 receptor and IFNγ expression to induce the JAK1/STAT1 signaling pathway.

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Keywords

Prdx6; human epididymis epithelial cells; RNAi; DGE; JAK1; STAT1

About this article
Title

Activation of the JAK1/STAT1 signaling pathway is associated with peroxiredoxin 6 expression levels in human epididymis epithelial cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 60, No 3 (2022)

Article type

Original paper

Pages

226-236

Published online

2022-08-04

Page views

4231

Article views/downloads

670

DOI

10.5603/FHC.a2022.0021

Pubmed

35929062

Bibliographic record

Folia Histochem Cytobiol 2022;60(3):226-236.

Keywords

Prdx6
human epididymis epithelial cells
RNAi
DGE
JAK1
STAT1

Authors

Hui Shi
Xiaoyu Liu
Yanwei Wang
Haiyan Wang
Bochen Pan
Jianyuan Li

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