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Vol 60, No 2 (2022)
Original paper
Submitted: 2021-10-27
Accepted: 2022-06-10
Published online: 2022-06-22
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MIR4435-2HG, miR-125b-5p, and Sema4D axis affects the aggressiveness of colorectal cancer cells

Ming Yu1, Zhengguo Yi1, Shenghui Chen1, Xiaopeng Chen1, Xiaofeng Xie1
DOI: 10.5603/FHC.a2022.0018
·
Pubmed: 35730423
·
Folia Histochem Cytobiol 2022;60(2):191-202.
Affiliations
  1. Department of General Surgery (Two), Shangrao Municipal Hospital, Shangrao, Jiangxi 334000, P.R. China

open access

Vol 60, No 2 (2022)
ORIGINAL PAPERS
Submitted: 2021-10-27
Accepted: 2022-06-10
Published online: 2022-06-22

Abstract

Introduction. The purpose of this study is to elucidate the impact of long non-coding RNA (lncRNA) MIR4435-2HG/microRNA (miR)-125b-5p/ Semaphorins 4D (Sema4D) on colorectal cancer (CRC) cell propagation and migration.
Material and methods. Sema4D expression in 73 pairs of CRC tissues and matched adjacent normal tissues was measured by qRT-PCR and western blot and its association with pathological characteristics of CRC patients was analyzed by chi-square test. Also, the expression of MIR4435-2HG, miR-125b-5p and Sema4D in CRC cell lines was detected by qRT-PCR and Western blot. Knockdown or overexpression of MIR4435-2HG, miR-125b- 5p and Sema4D were separately performed in Caco-2 and LoVo cells, and the cell propagation, migration and invasiveness were detected by cell-counting kit 8, scratch, and transwell assays.
Results. LncRNA MIR4435-2HG and Sema4D were highly expressed, while miR-125b-5p expression was decreased in CRC tissues and cells. Knockdown of MIR4435-2HG/Sema4D or overexpression of miR-125b-5p inhibited CRC cell proliferation and aggressiveness; overexpression of MIR4435-2HG/Sema4D or knockdown of miR-125b-5p prompted the malignant behaviors of cancer cells. MIR4435-2HG and Sema4D competitively bound to miR-125b-5p.
Conclusions. LncRNA MIR4435-2HG targets miR-125b-5p to upregulate Sema4D expression, and thus regulates CRC cell propagation, migration and invasiveness.

Abstract

Introduction. The purpose of this study is to elucidate the impact of long non-coding RNA (lncRNA) MIR4435-2HG/microRNA (miR)-125b-5p/ Semaphorins 4D (Sema4D) on colorectal cancer (CRC) cell propagation and migration.
Material and methods. Sema4D expression in 73 pairs of CRC tissues and matched adjacent normal tissues was measured by qRT-PCR and western blot and its association with pathological characteristics of CRC patients was analyzed by chi-square test. Also, the expression of MIR4435-2HG, miR-125b-5p and Sema4D in CRC cell lines was detected by qRT-PCR and Western blot. Knockdown or overexpression of MIR4435-2HG, miR-125b- 5p and Sema4D were separately performed in Caco-2 and LoVo cells, and the cell propagation, migration and invasiveness were detected by cell-counting kit 8, scratch, and transwell assays.
Results. LncRNA MIR4435-2HG and Sema4D were highly expressed, while miR-125b-5p expression was decreased in CRC tissues and cells. Knockdown of MIR4435-2HG/Sema4D or overexpression of miR-125b-5p inhibited CRC cell proliferation and aggressiveness; overexpression of MIR4435-2HG/Sema4D or knockdown of miR-125b-5p prompted the malignant behaviors of cancer cells. MIR4435-2HG and Sema4D competitively bound to miR-125b-5p.
Conclusions. LncRNA MIR4435-2HG targets miR-125b-5p to upregulate Sema4D expression, and thus regulates CRC cell propagation, migration and invasiveness.

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Keywords

colorectal cancer; humans; cell lines; proliferation; migration; invasion; survival analysis

About this article
Title

MIR4435-2HG, miR-125b-5p, and Sema4D axis affects the aggressiveness of colorectal cancer cells

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 60, No 2 (2022)

Article type

Original paper

Pages

191-202

Published online

2022-06-22

Page views

4541

Article views/downloads

586

DOI

10.5603/FHC.a2022.0018

Pubmed

35730423

Bibliographic record

Folia Histochem Cytobiol 2022;60(2):191-202.

Keywords

colorectal cancer
humans
cell lines
proliferation
migration
invasion
survival analysis

Authors

Ming Yu
Zhengguo Yi
Shenghui Chen
Xiaopeng Chen
Xiaofeng Xie

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