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Application of adipose mesenchymal stem cell-derived exosomes-loaded β-chitin nanofiber hydrogel for wound healing
Affiliations- National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, China
- Emergency Medicine Department of General Hospital of Northern Theater Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang, China
- Shenyang Medical College, Shenyang, China
- The Second Affiliated Hospital of Shenyang Medical College, the Veterans General Hospital of Liaoning Province, Shenyang, China
- Jihua Laboratory, Foshan, China
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Abstract
Introduction. Clarifying the role and mechanism of exosome gel in wound repair can provide a new effective strategy for wound treatment. Materials and methods. The cellular responses of adipose mesenchymal stem cell-derived exosomes (AMSC-exos) and the wound healing ability of AMSC-exos-loaded β-chitin nanofiber (β-ChNF) hydrogel were studied in vitro in mouse fibroblasts cells (L929) and in vivo in rat skin injury model. The transcriptome and proteome of rat skin were studied with the use of sequenator and LC-MS/MS, respectively. Results. 80 and 160 μg/mL AMSC-exos could promote the proliferation and migration of mouse fibroblastic cells. Furthermore, AMSC-exos-loaded β-ChNF hydrogel resulted in a significant acceleration rate of wound closure, notably, acceleration of re-epithelialization, and increased collagen expression based on the rat full-thickness skin injury model. The transcriptomics and proteomics studies revealed the changes of the expression of 18 genes, 516 transcripts and 250 proteins. The metabolic pathways, tight junction, NF-κB signaling pathways were enriched in Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway. Complement factor D (CFD) and downstream Aldolase A (Aldoa) and Actn2 proteins in rats treated with AMSC-exos-loaded β-ChNF hydrogel were noticed and further confirmed by ELISA and Western blot. Conclusion. These findings suggested that AMSC-exos-loaded β-ChNF hydrogel could promote wound healing with the mechanism which is related to the effect of AMSC-exos on CFD and downstream proteins.
Abstract
Introduction. Clarifying the role and mechanism of exosome gel in wound repair can provide a new effective strategy for wound treatment. Materials and methods. The cellular responses of adipose mesenchymal stem cell-derived exosomes (AMSC-exos) and the wound healing ability of AMSC-exos-loaded β-chitin nanofiber (β-ChNF) hydrogel were studied in vitro in mouse fibroblasts cells (L929) and in vivo in rat skin injury model. The transcriptome and proteome of rat skin were studied with the use of sequenator and LC-MS/MS, respectively. Results. 80 and 160 μg/mL AMSC-exos could promote the proliferation and migration of mouse fibroblastic cells. Furthermore, AMSC-exos-loaded β-ChNF hydrogel resulted in a significant acceleration rate of wound closure, notably, acceleration of re-epithelialization, and increased collagen expression based on the rat full-thickness skin injury model. The transcriptomics and proteomics studies revealed the changes of the expression of 18 genes, 516 transcripts and 250 proteins. The metabolic pathways, tight junction, NF-κB signaling pathways were enriched in Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway. Complement factor D (CFD) and downstream Aldolase A (Aldoa) and Actn2 proteins in rats treated with AMSC-exos-loaded β-ChNF hydrogel were noticed and further confirmed by ELISA and Western blot. Conclusion. These findings suggested that AMSC-exos-loaded β-ChNF hydrogel could promote wound healing with the mechanism which is related to the effect of AMSC-exos on CFD and downstream proteins.
Keywords
Adipose mesenchymal stem cell-derived exosomes; β-chitin nanofiber; rat skin; wound healing; transcriptomics; proteomics
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About this articleTitle
Application of adipose mesenchymal stem cell-derived exosomes-loaded β-chitin nanofiber hydrogel for wound healing
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
167-178
Published online
2022-05-30
Page views
5516
Article views/downloads
1098
DOI
Pubmed
Bibliographic record
Folia Histochem Cytobiol 2022;60(2):167-178.
Keywords
Adipose mesenchymal stem cell-derived exosomes
β-chitin nanofiber
rat skin
wound healing
transcriptomics
proteomics
Authors
Ying Liu
Yunen Liu
Yan Zhao
Mi Wu
Shun Mao
Peifang Cong
Rufei Zou
Mingxiao Hou
Hongxu Jin
Yongli Bao
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